Inhibitory Activity of Piper Caninum Leaf Extract against Curvularia Spotting Disease on Rice Plants

DOI: 10.18805/IJARe.A-560    | Article Id: A-560 | Page : 411-419
Citation :- Inhibitory Activity of Piper Caninum Leaf Extract against Curvularia Spotting Disease on Rice Plants.Indian Journal Of Agricultural Research.2020.(54):411-419
Ni Luh Suriani, Dewa Ngurah Suprapta, Novizar Nazir, Anak. Agung. Ketut Darmadi, Ni Made Susun Parwanayoni, Ni. Wayan Sudatri, Bohari M. Yamin niluhsurianisuriani@yahoo.com
Address : Udayana University, Bukit Jimbaran, Badung, Bali, Indonesia.
Submitted Date : 7-04-2020
Accepted Date : 5-06-2020

Abstract

Rice is a plant that has been intensively breed, especially in Asia, most of the population’s staple food is rice. There were Many types of rice varieties have been developed for high yeald production. Different rice diseases arise that many treat to reduce production. Such as curvularia spotting disease caused by the fungus Curvularia sp. Infection of Curvularia species causing leaf spots, leaf blights, kernel root, grain deformation and other diseases are well known globally. It was discovered that the small brown oval spots on the Ciherang rice variety cultivated in Bali, Indonesia was caused by Curvularia verruculosa. This disease could reduce the rice yield up to almost 50%. The application of commercial fungicide by the local farmers has not been satisfactorily successful in term of controlling the disease but instead bring damages to the environment and the soil as well. Farmer’s safety is also another serious issue. The current studi develop a natural and green fungicide to check the efficacy of Piper caninum extract against the fungi. Research in the laboratory uses a completely randomized design and research in the field uses a randomized group design.The method of making leaf extracts uses maceration method. Diffusion well technique showed a strong inhibition of the abstract against the C. verruculosa with 22mm inhibition. Zone with a total inhibition of 4% extract concentration. The minimum inhibitory concentration (MIC) was low of 0.5%. Scanning electron images of the treated fungi showed hollow and wrinkled mycelia morphology indicating lysis of the cell has occurred. P. caninum leaf extract at a concentration of 3% provides the highest obstacle to the intensity of C. verruculosa disease, whereas in the treatment of leaf extract concentration p. caninum 4% intensity of C. verrucuosa disease has decreased because rice plants are toxic at a concentration of 4%. Further research is needed regarding the treatment of P. caninum leaf extract against C. verruculosa in rice fields.

Keywords

Curvularia verrucuosa Piper caninum Rice Scanning electron images

References

  1. Alkooranee, J.T. Al-khshemawee, H.G. badri, M.A.K.L. Al-srai, M.S. Daweri, H.H. (2020). Antifungal activity and GC-MS detection of leaves and roots parts of Chenopodium album extract against some phytopathogenic fungi. Indian Journal of Agricultural Research. 54(1): 117-121.
  2. Almaguer, M., A. Batista and V.D. Amador. (2012). Effect of Temperature on Growth and Germination of Conidia in Curvularia and Bipolaris Species Isolated from the Air. Aerobiologia. Springer Science: London.
  3. Astiti, N.P.A. and Suprapta, D.N. (2012). Antifungal Activity of Teak (Tectona grandis L.F) Leaf Extract Against Arthrinium Phaeospermum (Corda) M.B. Ellis. The Cause of Wood Decay on Albizia Falcataria (L.) Fosberg. J.ISSAAS. 18 (1): 62-69.
  4. Chouhan, S.K. Sharma and S. Guleria, (2017). Antimicrobial Activity of Some Essential Oils-Present Status and Future Perspectives. Medicines. 4(58): 1-20.doi:10.3390/ medicines 4030058: 
  5. Darmadi. A.A.K, Suprapta. DN, Ginantra. I.K. (2016). Effect of leaf extract Formula Cinnamomum burmanni Blume on of Fussarium oxysporrum wilt that Attacks Tomato Plants in Bali. International Journal Pure and applied Bioscience. 4(4): 33-38.
  6. Darmadi. A.A.K, Suprapta. DN, Temaja. R.M.T, Swantara. I.M.D. (2015). Leaf Extract of Cinnamomum burmanni Blume Effectively suppress the growth of Fussarium oxysporrum wilt desease on tomato. Journal of Biologi agriculture and Healthcare. 5(4):131-137.
  7. Darmadi. A.A.K, Suprapta. DN, Temaja. R.M.T, Swantara. I.M.D. (2017). Gc-Ms Analysis of Active Compound of Cinnamomum Leaf Extract. International Journal of Pharma and Bio Sciences. 9(1): 114-118. 
  8. Fumitaka. S, Nobuo. S, Naumi. A, Suprapta. D.N, Nurwulan. A, Youji. N and Masakazu, K. (2015). Kinitiation and Dissemination of Organic Rice Cultivation in bali, Indonesia. Sustainability. 7(5): 5171-5181.
  9. Gentallan Jr. Altoveros, N.C. Borromeo, T.H and. Macabecha, C.G.A. (2019). Antimicrobial and Phytochemical Properties of Atuna racemosa Raf. Kernel Extract. Indian J. Agric. Res. 53(6): 733-736. 
  10. Harborne, J.B. (1987). Metode Fitokimia. Penuntun Cara Modern Menganalisis Tumbuhan (terjemahan). Padmawinata, K., Soediro, I., penerjemah. Bandung: ITB.
  11. Hidayat, J, Margaret, A.L. Hanna, Y. and Lies K. W. (2013). Isolates of H-Hexane Extract of Azadirachta indica A. Jus (Neem) Leaves. J. Nature and Science. 11(5):141-147.
  12. Kaaniche, F. Abdelaaty, H. Ahmed, S. Abdel,R. Daniel, W. Negera, A. Imene, Z.E.l, Noureddine Allouche2, Lotfi Mellouli3, Mohamed Shaaban1,8, Nobert Sewald. (2019). Bioactive secondary metabolites from new endophytic fungus Curvularia. sp isolated from Rauwolfia macrophylla.PLOS ONE. 6 (27): 1-12. | https://doi.org/10.1371/journal. pone.0217627.
  13. Kawuri, R. (2012). “Pemanfaatan Streptomyces thermocarboxydus untuk Mengendalikan Penyebab Penyakit Busuk Daun pada Lidah Buaya (Aloe barbadensis Mill.) Di Bali” (desertasi). Denpasar: Universitas Udayana.
  14. Kithan, C and Daiho L. (2014). In Vitro Evaluation of Botanicals, Bio-Agents and Fungicides against Leaf Blight of Etlingera linguiformis Caused by Curvularia lunata Var. Aeria. J Plant Pathol Microbiol an open access Journal. 5(3): 1-6.
  15. Kumar. S, A.A Lal, N. Kumar, S.Jaiswal, H. Kumar, A. and Kumar, M. (2017). Effect of bio control agents and botanicals against Blast of Paddy caused by Pyricularia oryzae. International Journal of Chemical Studies. 5(1): 314-318. 2017.
  16. Kusai, N.A. Madihah, M.Z.A. Shahrizim, Z. ohd, O.T. Nur, A.I.M.Z. (2015). Morphological and molecular characterization of Curvularia and related species associated with leaf spot disease of rice in Peninsular Malaysia. Rendiconti Lincei. Scienze Fisiche e Naturali. 4(9): 1-10.
  17. Law W-F, H.L. Hooi-Leng Ser, T.M. Khan, L-H. Chuah, P. Pusparajah, K-G. Chan, B-H. Goh and L-H. Lee. (2017). The Potential of Streptomyces as Biocontrol Agents against the Rice Blast Fungus, Magnaportheoryzae (Pyricularia oryzae), Front Microbiol. 8(3). doi: 10.3389/fmicb.2017.00003. 
  18. Maj, Jones, S.H., Marshall, R., Johnson, R.K. and Hecht, S.M. (2004). A DNA-damaging oxoaporphine alkaloid from Piper caninum. Departments of Chemistry and Biology. University of Virginia. USA.
  19. Mezouari, A. Makhloufi, A. Bendjima, K. Benlarbi, L. Boulanouar, A. Makhlou, K and Jesùs Gonzalez, M.D. (2019). Antifungal activity of Acacia tortilis subsp. raddiana tar on Fusarium oxysporum f.sp. albedinis, the cause of Bayoud Disease of the date palm in Southwest Algeria. Indian J. Agric. Res. 53(6): 713-717.
  20. Mohana, D.C. Praveen, P, Veena, V. Koteshwara A.R. (2011). Plant Extract Effect on Seed-Borne Pathogenic Fungi from Seeds of Paddy Grown in Southern India. Journal of Plant Protection Research. 51 (2).
  21. Mondol, M.A.M. Jannatul, F. Mohammad, T.I. Anja, S. and Hartmu, L. (2017). Metabolites from the Endophytic Fungus Curvularia sp. M12 Act as Motility Inhibitors against Phytophthora capsici Zoospores. J. Nat. Prod. 80: 347-355. DOI:10. 1021/acs.jnatprod.6b00785.
  22. Noémi, K. Mónika, H. Palanisamy, M. Arumugam, M. Krisztina, K. Rajaraman, R. Mónika, V. Tamás, P. Csaba, V. László, K. and Sándor, K. (2020). New Species of the Genus Curvularia: C. tamilnaduensis and C. coimbatorensis from Fungal Keratitis Cases in South India. Pathogens. 9(9): 1-14. doi: 10.3390/pathogens9010009.
  23. Oraon, S. Mondal, S. (2020). Studies on Allelopathic Effect of Aqueous Leaf Extract of Putranjiva Roxburghii Wall. on Seed Germination and Early Growth of Chickpea (Cicer Arietinum L.). Indian Journal of Agricultural Research. 54(2): 193-198.0.
  24. Rajat. G, Katon. D, Payel. N, Panchali. D. (2014). An Overview piper Species for their Biological Activities. International Journal of Pharma research. 3(1):67-75.
  25. Randrianariveloa, R.S. Sarterb, E. Odouxc, P. Bratc, M. Lebrunc, B. Romestandd, C. Menute, H.S. Andrianoelisoaf, M. Raherimandimbyg and P. Danthuh, (2009). Composition and antimicrobial activity of essential oils of cinnanosma fragrans. Food Chems. 1(14): 680-684. 
  26. Razu, M.A.U. and I. Hossain. (2015). Eco-friendly management of rice diseases. International Journal Appl. Sci Biotechnol. 3(1): 80-88.
  27. Salleh, W.M.N.H.W., Ahmand, F., Yen. K.H. (2011). Chemical Compositions, Antioxidant and Antimicrobial Activities of Essential Oils of Piper caninum Blume. Int J Mol Sci. 12 (11): 7720-7731.
  28. Salleh, W.M.N.H.W., Ahmand, F., Yen. K.H. (2015). Chemical Constituens from Piper caninum Antibacterial Activity. Journal of Applied Pharmaceutical Science. 5(06): 20-25. 
  29. Sena, A.P.A., Amanda, A., Chaibub; Marcio, V.C.B., Cortes., Siva, G.B., Loho, V, L, S., Prabu, A,S., Filippi, M.C.C; Arauj, L.G. (2013). Increased Enzymatic Activity in Rice Leaf Blast Suppression by Crude Extract of Epicoccum sp. International Journal of Trop. Plant Pathol. 38(5): 23-42.
  30. Suprapta, D.N and Khalimi, K. (2012). Anti-fungal Activities of Selected Tropical Plants from Bali Island. J. Phytopharmacology. 2(2): 265-270. 
  31. Suprapta, D.N. PestisidaNabati Potensi dan Prospek Pengembangan. (2014). Pelawa Sari. Denpasar. 
  32. Suriani, N. L. (2019). Piper caninum blume leaf extract and compost to suppress blast disease and increase the production of bali red rice (Oryza sativa) in green house. International Research Journal of Engineering, IT and Scientific Research. 5(4): 46-54. https://doi.org/10.21744/irjeis.v5n4.693. 
  33. Suriani, N.L. (2019). Utilization of piper caninum blume leaf extract combined with compost to suppress blast disease and increase the growth of local rice Bali (Oryza sativa) in Vivo. International Journal of Life Sciences. 3(2): 33-40. https://doi.org/10.29332/ijls.v3n2.305.
  34. Suriani, N.L, Darmadi, A.A.K. Parwanayoni N.M.S. (2019). Hamid. The combination of Piper caninum Blume Leaf Extract and Compost Fertilizer for Pressing Blast Disease and Improving Growth of bali Red Rice (Oryzasativa Linn). IJASEIT Journal. (2): 518-525.
  35. Suriani, N.L. Darmadi, A.A.K. Suprapta, D.N. Khalimi, K. and Parwanayoni, N.M.S. 2019). The Combination of Piper Caninum Blume and Piper Betle Var. Nigra Leaf Extract to Press Blast Disease on Oryza Sativa Linn in Vitro. The Journal of Research on the Lepidoptera. 50(4): 196-204.
  36. Suriani, N.L. Darmadi, A.A.K. Suprapta, D.N. Khalimi, K. and Parwanayoni, N.M.S. Nazir, N, Ahma, Z.B.Z. Bohari, M.Y. (2019). Antagonism trichoderma sp for pressing blast disease on red bali rice plants (oryza sativa). Journal of Adv Research in Dynamical and Control Systems. 11(10): 70-76.
  37. Suriani. N.L, D.N.Suprapta, I.M. Sudana.I. M and R. M. Temaja, (2015). Antifungal activity of Piper caninum against Pyricularia oryzae Cav. The cause of rice blast disease on rice. International Journal ISTE. 5(8):72-78.
  38. Suriani. N.L. (2016). Identification of the Substance Bioactive Leaf Extract Piper caninum Potential as Botanical Pesticides. International Journal IJPAB. 4(4):26-32.
  39. Suriani. N.L. (2018). Bioactive Substance use of Leaf Extract of Piper caninum Blume Pressing for Blast Disease and Increase Production in Rice. International Journal IJLS. 2(2): 42-50.
  40. Susun. N.M.P, Suprapta. DN, Temaja. R.M.T, Swantara. I.M.D, Khamdan. K. (2018). Synergistic Activity of Leaves Extracts of Mansoa alliacea L. and Allamanda cathartica L. to Inhibit Athelia rolfsii, the Cause of Stem Rot Disease in Peanut Plants. Journal of Biologi agriculture and Healthcare. 8(4): 29-35.
  41. Tanjung, W.A.S. (2013). Obat Tradisional dan Metabolit Sekunder. Fakultas Biologi UGM. Yogyakarta.
  42. Tann, H and Soytong, K. (2017). Biological Control of Brown Leaf Spot Disease Caused by Curvularia lunata and Field Application Method on Rice Variety IR66 in Cambodia. AGRIVITA Journal of Agricultural Science. 39(1): 111-117.
  43. Upadhyay, A., Upadhyaya, I. Kollanoor-Johny, A and Venkitanarayanan, K. (2014). Combating pathogenic microorganisms using plant-derived antimicrobials. Hindawi Publishing Corporation BioMed Research International. 1-12. ID 761741 DOI: 10.1155 / 2014/761741.
  44. Vasantha Kumari, K. Shanmugam, P.M. (2020). Evaluation of Rice (Oryza Sativa) Varieties Suitable for Organic Farming. Indian Journal of Agricultural Research. 54(1): 71-76.
  45. Wan. M.N.H.W.S, Farediah. A, Khong. H.Y. Chemical constituents from Piper caninum and Antibacterial Activity. (2015). Journal of Applied Pharmaceutical Science. 5(06):20-36.
  46. Yin, C. Liping, J. Feifei, S.Xiao, X. Mingwei, S. and Ying, l. (2018). Phytotoxic and Antifungal Metabolites from Curvularia crepinii QTYC-1 Isolated from the Gut of Pantala flavescens. Molecules. 23(951): 1-9. doi: 10.3390/molecules23040951.
  47. Yin. L, Shuang. F.R, Jayarama. B, Kevin. D.H, Yong. W, De. G.Z. (2018). Curvularia microspora sp. Nov. Associated with Leaf Diseases of Hippeastrum Striatum in china. Journal Mycokeys. 29: 49-61.
  48. Yue. HW, Susan. L, Morris.N, Jun. Y, Hong. M.N, Chun. L.L, Kuo. H.L. (2014). Anticancer principles from Medicinal Piper Plants. Journal of Traditional and Complementary Medicinal. 4(1): 8-16.

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