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Isolation, Characterization of Phosphatase Producing Bacteria and Fungi-Their Effect on Enhancing the Growth of Vigna radiata

DOI: 10.18805/IJARe.A-5588    | Article Id: A-5588 | Page : 396-402
Citation :- Isolation, Characterization of Phosphatase Producing Bacteria and Fungi-Their Effect on Enhancing the Growth of Vigna radiata.Indian Journal of Agricultural Research.2021.(55): 396-402
Bandi Aruna, Sejal Jain kaviliaruna@gmail.com
Address : Department of Microbiology, St. Francis College for Women, Begumpet, Hyderabad-500 016, Telangana, India.
Submitted Date : 22-04-2020
Accepted Date : 1-09-2020

Abstract

Background: Phosphate solubilizing microorganisms (PSM) are considered as most important traits associated with plant phosphate nutrition and growth. The challenge of study include identification of potent microorganisms that can be used as phosphate solubilizer.
Methods: In this study during 2017-2018 study nine isolates were isolated from soil, dung, lakes, five bacterial isolates (A, B, C, D, E) identified as, Bacteroides sp, four fungal isolates Aspergillus sp (F1) and Mucor sp (F2, F3, F4). Their effect on plant (Vigna radiata) growth was studied. Maximum phosphatase production was observed by B (266 µg/ml) and F2 (297.5 µg/ml) isolates; and also phosphate by B (10.6 µg/ml) and F2 (42.6 µg/ml). Increase in shoot length and number of roots was observed in the presence of isolates.
Result: The contribution of study indicate, phosphate solubilizing isolates, have probable application as biofertilizer and may help in future agricultural needs.

Keywords

Biofertilizer Phosphate solubilizing microorganisms Pikovskaya Phosphatase Vigna radiata

References

  1. Agnihotri, V.P. (1970). Solubilization of insoluble phosphates by some fungi isolated from nursery seedbeds. Canadian Journal of Microbiology. 16: 877-880. 
  2. Arcand, M.M. and Schneider, K.D. (2006). Plant-and microbial-based mechanisms to improve the agronomic effectiveness of phosphate rock: A review. Anais da Academia Brasileira de Ciências. 78: 791-807. 
  3. Bashan, Y. and Holguin, G. (1997). Azospirillum-plant relationships: environmental and physiological advances (1990-1996). Canadian Journal of Microbiology. 43: 103-121. 
  4. Behera, B., Yadav, H., Singh, S., Mishra, R., Sethi, B., Dutta, S. (2017). Phosphate solubilization and acid phosphatase activity of Serratia sp. isolated from mangrove soil of Mahanadi river delta, Odisha. India. Journal of Genetic Engineering and Biotechnology. 15: 169-178. 
  5. Chen, W., Yang, F., Zhang, L., Wang, J.  (2016b) Organic acid secretion and phosphate solubilizing efficiency of Pseudomonas sp. PSB12: effects of phosphorus forms and carbon sources. Geomicrobiology Journal. 33: 870- 877. 
  6. Dave, A. and Patel, H.H. (1999). Inorganic phosphate solubilizing Pseudomonas. Indian Journal of Microbiology. 39: 161-164. 
  7. Huang, J., Sheng, X. and He, L. (2010). Biodiversity of phosphate-dissolving and plant growth promoting endophytic bacteria of two crops. Acta Microbiologica Sinica. 50: 710-716. 
  8. Ingham, E., Holland, K.T., Gowland, G. and Cunliffe, W.J. (1979). Purification and partial characterization of an acid phosphatase produced by Propionibacterium acnes. Journal of General Microbiology. 118: 59-65. 
  9. Jackson, M. (1973). Soil chemical analysis. Prentice Hall of India, New Delhi, India. 
  10. Karnavat R., Pavaya, R.P., Malav, J.K., Neha Chaudhary, Patel, I.M., Patel, J.K. (2018). Effect of FYM, phosphorus and PSB on yield, nutrient content and uptake by green gram [Vigna radiata (L.)Wilckzek] on loamy sand. International Journal of Chemical Studies. 6: 1026-1029. 
  11. Kim, K.Y., Jordan, D., McDonald, G.A. (1997). Solubilization of hydroxyapatite by Enterobacter agglomerans and cloned Escherichia coli in culture medium. Biology and Fertility of Soils. 24: 347- 352.
  12. Lal, L. (2002). In: Phosphate mineralizing and solubilizing micro-rganisms. Phosphatic Biofertilizers. Agrotech Publishing Academy, Udaipur. p. 224.
  13. Lowry, O.H., Rosebrought, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin phenol reagent, Journal of Biological Chemistry. 193: 265-75. 
  14. Mohinder, K.,, Sapna, S., Atul Mishra. (2011). Influence of phosphate solubilizing Pseudomonas and Bacillus strains on the growth of Ashvagandha (Withania somnifera). Indian Journal of Agricultural Research. 45:128-133.
  15. Nahas, E. (1996). Factors determining rock phosphate solubilization by microorganisms isolated from soil. World Journal of Microbiology and Biotechnology. 12: 567-572. 
  16. Nautiyal, C.S., Bhadauria, S., Kumar, P., Lal, H., Mond, L.R. and Verma, D. (2000). Stress induced phosphate solubilization in bacteria isolated from alkaline soils. FEMS Microbiological Letters. 182: 291-296. 
  17. Onyia, C.E., Anyawu, C.U., Ikegbunam, M.N. (2015). Ability of fungi, isolated from nsukka peppers and garden-egg plant rhizospheres, to solubilize phosphate and tolerate cadmium. Advances in Microbiology. 5: 500-506. 
  18. Ponmurugan, P. and Gopi, C. (2006). In vitro production of growth regulators and phosphatase activity by phosphate solubilizing bacteria. African Journal of Biotechnology. 5: 348-350. 
  19. Porschen, R.K. and Spaulding, E.H. (1974). Phosphatase activity of anaerobic organisms. Applied Microbiology. 27: 744-747.
  20. Qian Chen and Shanjiang Liu. (2019). Identification and Characterization of the Phosphate Solubilizing Bacterium Pantoea sp. S32 in Reclamation Soil in Shanxi, China. Frontiers in Microbiology. 10: 1-12. Article 2171. 
  21. Otieno, N., Lally, R.D., Kiwanuka, S., Lloyd, A., Ryan, D., Germaine, K.J. (2015). Plant growth promotion induced by phosphate solubilizing endophytic Pseudomonas isolates. Frontiers in Microbiology. 6: 745. 
  22. Rodrigue, H. and Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances. 17: 319-339. 
  23. Rosso, L., Lobry, J.R., Bajard, S. and Flandrois, J.P. (1995). Convenient model to describe the combined effects of temperature and pH on microbial growth. Applied and Environmental. Microbiology. 61: 610- 616. 
  24. Rudek, W. and Haque, R.U. (1976). Extracellular enzymes of the genus Bacteroides. Journal of Clinical Microbiology. 4: 458-460. 
  25. Sarkar, M.C. and Uppal, K.S. (1994). Phosphorus research in India. Potash and Phosphate Institute of Canada. India Programme, Gurgaon, Haryana, India. 
  26. Seshagiri, S. and Tallapragada, P. (2016). Study of Acid Phosphatase in Solubilization of Inorganic Phosphates by Piriformospora indica. Polish Journal of Microbiology. 65: 407-412.
  27. Skerman T.M. (1975). Determination of some in vitro growth requirements of Bacteroides nodosus; Journal of General Microbiology. 87: 107-119. 
  28. Song, Y., Liu, C., Bolanos, M., Lee, J., McTeague, M., Finegold, S.M. (2005). Evaluation of 16S rRNA sequencing and reevaluation of a short biochemical scheme for identification of clinically significant Bacteroides species. Journal of Clinical Microbiology. 43: 1531-1537. 
  29. Suhana, P.G., Maurya, B.R., Akhila N.D., Nitesh K.S. (2019). Role of phosphorus solubilizing microorganisms and dissolution of insoluble phosphorus in soil. International Journal of Chemical Studies. 7: 3905-3913.
  30. Sullivan P. (2001). Alternative soil amendments. Appropriate Technology Transfer for Rural Areas, National Centre for Appropriate Technology.  
  31. Tarafdar, J.C., Rao, A.V. and Praveen Kumar. (1992). Effects of different phosphatase-producing fungi on growth and nutrition of Mung beans in an arid soil. Biology and Fertility of Soils. 13: 35-38. 
  32. Tingting, Z., Feng, H., Lei, M. (2019). Phosphate-solubilizing bacteria from safflower rhizosphere and their effect on seedling growth. Open Life Sciences. 14: 246-254. 
  33. Tyagi, M.K., Singh, C.P., Bhattacharayya, P., Sharma, N.L. (2003). Indigenous low-grade rock phosphate utilized efficiently as an alternate source of SSP with the use of Rhizobium and PSB. Indian Journal of Agricultural Research. 37: 1-8.
  34. Xiao-Kai, S., Juan-Juan, M., Li-Jun, L. (2017). Effects of phosphate-solubilizing bacteria application on soil phosphorous availability in coal mining subsidence area in Shanxi. Journal of Plant Interactions. 12: 137-142. 
  35. Zahoor, A.B., Sheikh, T.A., Basharat, H. (2014). Functional diversity of culturable phosphate solubilizing fluorescent Pseudomonads. Indian Journal of Agricultural Research. 48: 472-479.

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