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A Comprehensive Study on IAA production by Bradyrhizobium japonicum and Bacillus subtilis and Its Effect on Vigna radiata Plant Growth

DOI: 10.18805/IJARe.A-5521    | Article Id: A-5521 | Page : 570-576
Citation :- A Comprehensive Study on IAA production by Bradyrhizobium japonicum and Bacillus subtilis and Its Effect on Vigna radiata Plant Growth.Indian Journal of Agricultural Research.2021.(55):570-576
S. Kiruthika, M. Arunkumar kiruthikasundar1210@gmail.com
Address : PG and Research Department of Microbiology, PSG College of Arts and Science, Coimbatore-641 014, Tamil Nadu, India. 
Submitted Date : 7-02-2020
Accepted Date : 1-09-2020

Abstract

Background: The use of chemical fertilizers and pesticides raises concerns about environmental pollution, health hazards and the destruction of biotic groups that support plant growth. Plant growth-promoting rhizobacteria (PGPR) thrive in the rhizosphere of plants are the auspicious alternative for these chemicals. PGPR plays a critical role in plant growth and development, along with biocontrol activities. 
Methods: In this present study, two effective microbes, Bradyrhizobium japonicum and Bacillus subtilis were chosen and their ability to produce Indole Acetic acid (IAA) was determined. Optimization of IAA production was carried out in different cultural conditions. Further, in-vitro studies were carried out to analyze the effect of these bacteria on the growth of Vigna radiata
Results: Our investigations showed that both organisms have the potential to produce IAA under standard conditions. IAA production is maximum when using Bradyrhizobium japonicum with the supplement of Carboxymethyl cellulose and yeast extract as C and N source, respectively. L-Tryptophan concentration has a positive effect on production. Further, the application of bacterial cultures has shown more significant improvement in plant growth in terms of root and shoot length and weight of crop material. The current findings recommend that Bradyrhizobium japonicum can be a suitable organism for application as a plant growth promoter.

Keywords

Bacillus subtilis Bradyrhizobium japonicum Indole acetic acid PGPR Pot assay

References

  1. Agbodjato, N.A., Noumavo, P.A., Baba-Moussa, F., Salami, H.A., Sina, H., Sèzan, A. and Baba-Moussa, L. (2015). Characterization of potential plant growth promoting rhizobacteria isolated from Maize (Zea mays L.) in central and Northern Benin (West Africa). Applied and Environmental Soil Science. vol. 2015, Article ID 901656, 9 pages, 2015. https://doi.org/10.1155/2015/901656
  2. Ahemad, M. and Khan, M.S. (2012). Productivity of green gram in tebuconazole-stressed soil, by using a tolerant and plant growth-promoting Bradyrhizobium sp. MRM6 strain. Acta Physiologiae Plantarum. 34(1): 245-254.
  3. Ansari, F.A. and Ahmad, I. (2018). Biofilm development, plant growth promoting traits and rhizosphere colonization by Pseudomonas entomophila FAP1: A Promising PGPR. Advances in Microbiology. 8(03): 235.
  4. Bhowmick, P.K. and Basu, P.S. (1986). Production of indole acetic acid by Rhizobium sp. from root nodules of the leguminous tree Sesbania grandiflora pers. Acta Microbiologica Polonica. 35: 181-190.
  5. Cappucino, J.C. and Sherman, N. (1992). Microbiology: A Laboratory Manual, 3rd Edition. New York: Benjamin/Cummings Pub. 134: 125-179.
  6. Chaudhary, S.R. and Sindhu, S.S. (2016). Growth stimulation of clusterbean (Cyamopsis tetragonoloba) by coinoculation with rhizosphere bacteria and Rhizobium. Legume Research-An International Journal. 39(6): 1003-1012.
  7. Das, A. J., Kumar, M. and Kumar, R. (2013). Plant growth promoting rhizobacteria (PGPR): an alternative of chemical fertilizer for sustainable, environment friendly agriculture. Res. J. Agric. For Sci. 1(4): 21-23.
  8. Etesami, H. and Beattie, G.A. (2018). Mining halophytes for plant growth-promoting halotolerant bacteria to enhance the salinity tolerance of non-halophytic crops. Frontiers in Microbiology. 9: 148.
  9. Frankenberger Jr., W.T., Arshad, M. (1995) Phytohormones in Soil: Microbial Production and Function. Marcel Dekker Inc., NY, USA, pp. 503 
  10. Gordon, S.A. and Weber, R.P. (1951). Colorimetric estimation of Indoleacetic acid. Plant Physiology. 26(1): 192.
  11. Kumar, A., Singh, M., Singh, P.P., Singh, S.K., Singh, P.K. and Pandey, K.D. (2016). Isolation of plant growth promoting rhizobacteria and their impact on growth and curcumin content in Curcuma longa L. Biocatalysis and Agricultural Biotechnology. 8: 1-7.
  12. Kumari, S., Prabha, C., Singh, A., Kumari, S. and Kiran, S. (2018). Optimization of Indole-3-Acetic Acid Production by Diazotrophic B. subtilis DR2 (KP455653), Isolated from Rhizosphere of Eragrostis cynosuroides.
  13. Lorck, H. (1948). Production of hydrocyanic acid by bacteria. Physiol. Plant. 1: 142-146.
  14. Mohite, B. (2013). Isolation and characterization of Indole acetic acid (IAA) producing bacteria from rhizospheric soil and its effect on plant growth. J. Soil Sci. Plant Nutr. 13(3): 638-649.
  15. Patel, T. and Saraf, M. (2017). Biosynthesis of phytohormones from novel rhizobacterial isolates and their in vitro plant growth-promoting efficacy. J. Plant Interact. 12(1): 480-487.
  16. Pikovskaya, R.I. (1948). Phosphate mobilization in soils as related to life processes of some microorganisms. Mikrobiologiya. 17: 362-370.
  17. Raval, V.H. and Saraf, M. (2020). Biosynthesis and purification of indole-3-acetic acid by halotolerant rhizobacteria isolated from Little Runn of Kachchh. Biocatalysis and Agricultural Biotechnology. 23: 101435.
  18. Saeid, A., Prochownik, E. and Dobrowolska-Iwanek, J. (2018). Phosphorus solubilization by Bacillus species. Molecules. 23(11): 2897.
  19. Schwyn, B. and Neilands, J.B. (1987). Universal chemical assay for the detection and determination of siderophores. Anal. Bio­chem. 160: 47-56. 
  20. Shaharoona, B., Arshad, M. and Zahir, Z.A. (2006). Effect of plant growth promoting rhizobacteria containing ACC-deaminase on maize (Zea mays L.) growth under axenic conditions and on nodulation in mung bean (Vigna radiata L.). Letters in Applied Microbiology. 42(2): 155-159.
  21. Shahid, M. and Khan, M.S. (2018). Cellular destruction, phytohormones and growth modulating enzymes production by Bacillus subtilis strain BC8 impacted by fungicides. Pestic. Biochem. Physiol. 149: 8-19.
  22. Singh, N. and Singh, G. (2018). Plant growth promoting rhizobacteria and Rhizobium combinations are the key to reduce dependence on phosphorus fertilizers in lentil-A review. Agricultural Reviews. 39(1): 76-81.
  23. Walia, M., Batra, N. and Goyal, S. (2014). Isolation and characterization of plant growth promoting rhizobacteria and their application in plant growth. Legume Research-An International Journal. 37(1): 72-78.

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