Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 54 issue 6 (december 2020) : 731-737

7, 8- Dihydroxyflavone, An Effective Natural Product Reduce Ralstonia solanacearum Populations and Control Tomato Bacterial Wilt

P. Nandhakumar, V.S. Vasantha, P. Veilumuthu, Godwin Christopher
1Department of Natural Products Chemistry, School of Chemistry, Madurai Kamaraj University, Madurai-625 021, Tamilnadu, India. 
Cite article:- Nandhakumar P., Vasantha V.S., Veilumuthu P., Christopher Godwin (2020). 7, 8- Dihydroxyflavone, An Effective Natural Product Reduce Ralstonia solanacearum Populations and Control Tomato Bacterial Wilt. Indian Journal of Agricultural Research. 54(6): 731-737. doi: 10.18805/IJARe.A-5479.

ABSTRACT

In this study, 7, 8- dihydroxyflavone (DHF) was isolated first time from medicinal plant Oroxylum indicum and its antibacterial potential against plant pathogen Ralstonia solanacearum was determined. The minimum inhibitory and bactericidal concentrations of DHF were 20 µg/mL and 80 µg/mL respectively. The biofilm formation and inhibition of DHF against R.solanacearum was investigated via scanning electron microscopy indicated that destruction of the cell wall. The inhibition of biofilm formation and molecular assays revealed that DHF binds lumen periplasm side of the outer membrane protein (EpsA) was identified. Moreover, DHF effectively reduced the bacterial wilt disease and control efficiency is as high as 89.10% at nine days after inoculation. Altogether, the antibacterial potential of DHF against R.solanacearum displayed effective agent can attenuates bacterial wilt disease in tomato plant.

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