Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 54 issue 6 (december 2020) : 708-715

Effects of Root-Knot Nematode Inoculums Densities on Morphological and Phytochemical Analysis of Selected Horse Gram Germplasm

Thomas Cheeran Amal, Palanisamy Karthika, Venkatachalam Balamurugan, Govindan Shanmugam, Subramaniam Selvakumar, Palanisamy Sundararaj, Krishnan Vasanth
1Department of Botany, School of Life Sciences, Bharathiar University, Coimbatore-641 046, Tamil Nadu, India.
Cite article:- Amal Cheeran Thomas, Karthika Palanisamy, Balamurugan Venkatachalam, Shanmugam Govindan, Selvakumar Subramaniam, Sundararaj Palanisamy, Vasanth Krishnan (2020). Effects of Root-Knot Nematode Inoculums Densities on Morphological and Phytochemical Analysis of Selected Horse Gram Germplasm. Indian Journal of Agricultural Research. 54(6): 708-715. doi: 10.18805/IJARe.A-5430.

ABSTRACT

Root-knot nematodes (Meloidogyne incognita; RKN) are important constraints on agricultural crops worldwide. Horse gram is a vulnerable pulse crop from the agriculture revolution exceptionally in southern India. This study intended to assess the resistance of four Horse gram accessions IC-1536, IC-1572, IC-1451 and IC-1115 selected from 100 accessions based on the morphological and biochemical scrutiny. Individual seedlings were transferred in the clay pots packed with sterile soil and inoculated with three doses (25, 50 and 100) of second stage juveniles of M. incognita. All nematode inoculums densities influenced the shoot length of plants, flowering and yield. The level of amino acid, proline, protein, total phenol and flavonoids contents were increased while carbohydrate and starch contents were decreased in all the varieties compared to the 15th day after inoculation. It is pointed out that M. incognita has not only impaired the plant growth but also biochemical parameters such as chlorophyll pigments, primary and secondary metabolites.

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