Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 54 issue 3 (june 2020) : 388-393

Genome Classification of Banana Cultivars Found in Manipur, India using IRAP and RAPD

S. Sureshkumar Singh, W. Robindro Singh, Romesh Sagolshemcha, Karuna Shrivastava
1Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli-791 109, Itanagar, Arunachal Pradesh, India.
Cite article:- Singh Sureshkumar S., Singh Robindro W., Sagolshemcha Romesh, Shrivastava Karuna (2020). Genome Classification of Banana Cultivars Found in Manipur, India using IRAP and RAPD. Indian Journal of Agricultural Research. 54(3): 388-393. doi: 10.18805/IJARe.A-5347.

ABSTRACT

The genome groups of 34 wild and cultivated banana samples collected from 9 districts of Manipur, India were analyzed using IRAP and RAPD. IRAP-PCR analysis of the banana genome revealed that 32 out of 34 banana samples have shown presence of multiple polymorphic bands in amplified products. There were 5 genome specific bands (2 for B genome and 3 for A genome) in all the samples analyzed by using IRAP marker. Analysis of A and B genome among plant samples using RAPD-PCR generated a total of 1425 polymorphic bands by six primers (OPF-6, OPX-6, OPK-12, RAPD-6, RAPD-14 and RAPD-17). The highest number of bands was generated by OPK12 (318) followed by RAPD6 (308 bands), OPX6 (263 bands), RAPD 17 (215 bands), RAPD 14 (165 bands) and OPF6 (156 bands) respectively. Among these primers, RAPD 14 displayed specific bands per sample thereby showing higher delineating power than other primers. 

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