Genome Classification of Banana Cultivars Found in Manipur, India using IRAP and RAPD

DOI: 10.18805/IJARe.A-5347    | Article Id: A-5347 | Page : 388-393
Citation :- Genome Classification of Banana Cultivars Found in Manipur, India using IRAP and RAPD.Indian Journal Of Agricultural Research.2020.(54):388-393
S. Sureshkumar Singh, W. Robindro Singh, Romesh Sagolshemcha, Karuna Shrivastava romesh.sagolshemcha@gmail.com
Address : Department of Forestry, North Eastern Regional Institute of Science and Technology, Nirjuli-791 109, Itanagar, Arunachal Pradesh, India.
Submitted Date : 26-07-2019
Accepted Date : 11-11-2019

Abstract

The genome groups of 34 wild and cultivated banana samples collected from 9 districts of Manipur, India were analyzed using IRAP and RAPD. IRAP-PCR analysis of the banana genome revealed that 32 out of 34 banana samples have shown presence of multiple polymorphic bands in amplified products. There were 5 genome specific bands (2 for B genome and 3 for A genome) in all the samples analyzed by using IRAP marker. Analysis of A and B genome among plant samples using RAPD-PCR generated a total of 1425 polymorphic bands by six primers (OPF-6, OPX-6, OPK-12, RAPD-6, RAPD-14 and RAPD-17). The highest number of bands was generated by OPK12 (318) followed by RAPD6 (308 bands), OPX6 (263 bands), RAPD 17 (215 bands), RAPD 14 (165 bands) and OPF6 (156 bands) respectively. Among these primers, RAPD 14 displayed specific bands per sample thereby showing higher delineating power than other primers. 

Keywords

RAP Musa acuminata Musa balbisiana RAPD

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