Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.20

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 55 issue 1 (february 2021) : 13-22

A Protocol for Rapid Clonal Propagation and Microrhizome Production of Curcuma caesia Roxb. (Zingiberaceae): A Critically Endangerd Medicinal Plant of North East India 

Indrani Sarma, A.C. Deka, T.C. Sarma
1The Energy and Resources Institute, North Eastern Regional Centre, Guwahati-781 036, Assam, India. 
Cite article:- Sarma Indrani, Deka A.C., Sarma T.C. (2020). A Protocol for Rapid Clonal Propagation and Microrhizome Production of Curcuma caesia Roxb. (Zingiberaceae): A Critically Endangerd Medicinal Plant of North East India. Indian Journal of Agricultural Research. 55(1): 13-22. doi: 10.18805/IJARe.A-5338.
Background: Curcuma caesia Roxb. (Zingiberaceae) is a rare, critically endangered medicinal plant of Northeast India. The rhizome of the plant is famous for its significant medicinal properties. 
Methods: Various concentrations of plant growth regulators in Murashige and Skoog (MS) medium were tried in the study using rhizome bud as explant for development of an efficient cost effective protocol for in vitro mass multiplication and microrhizome induction of Curcuma caesia. 
Result: Shoot multiplication and plant generation was achieved from freshly sprouted shoots of Curcuma caesia on Murashige and Skoog’s medium supplemented with different phytohormones. The best response for shoot multiplication (23.80±0.51shoots/explant) was obtained within 8 weeks in MS medium supplemented with BAP (1.0 mgl-1) and 2,4-D (0.25 mgl-1). Microrhizomes were induced at the base of the in vitro derived shoots upon transferred to medium containing various combinations and concentrations of sucrose and BAP and grown under varying photoperiod. Half strength of MS basal medium containing BAP (1.0 mg/l) and 9% sucrose was found to be optimum for induction of large sized microrhizome within 45 days of incubation under 16 hrs of photoperiod. 
  1. Abubakar, A.S. and Pudake, R.M. (2014). Curcuma caesia: A wonder herb with medicinal properties (review). Indian J. Scholarly Res. 3(6): 1-4
  2. Abubakar, A.S. and Pudake, R. M (2019). Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia). Agricultural Science Digest. 39(2): 96-101. 
  3. Arulmozhi DK, Sridhar N, Veeranjaneyulu A and Arora KS. (2006). Preliminary Mechanistic studies on the smooth muscle relaxant effect of hydroacloholic extract of Curcuma caesia Journal of Herbal Pharmacotherapy. 6: 3-4.
  4. Balachandran S.M.; Bhat, S.R. and Chandel, K.P.S. (1990). In vitro clonal multiplication of turmeric (Curcuma sp.) and ginger (Zingiber officinale Rosc.). Plant Cell Rep. 8: 521-524.
  5. Behar, N., Tiwari, K.L. and Jadhav, S.K. (2013). Comparative phytochemical screening of bioactive compounds in Curcuma caesia Roxb. and Curcuma longa. Research Journal of Medicinal Plant. 7(2): 113-118.
  6. Behura, S., Sahoo, S. and Srivastava, V.K. (2002). Major constituents in leaf essential oils of Curcuma longa L. and Curcuma aromatica Salisb. Curr. Sci. 83: 1312-1313.
  7. Bejoy, M.; Dan, M. and Anish, N.P. (2006). Factors Affecting the in vitro Multiplication of the Endemic Zingiber Curcuma haritha Mangaly and Sabu. Asian Journal of Plant Sciences. 5(5): 847-853.
  8. Borah A.R., Anbumalarmathi J. and Sharmili A.S. (2019). In vitro propagation of Coccinia indica (L.) Voigt. from internodal segments. Indian Journal of Agricultural Research. 53: 202-207.
  9. Borthakur, M., Hazarika, J. and Singh, R.S. (1999). A protocol for micropropagation of Alpinia galanga L., Plant Cell, Tissue and Organ Culture. 55: 231-233.
  10. Chan, L.K. and Thong, W.H. (2004). In vitro propagation of Zingiberaceae species with medicinal properties. J. Plant Biotechnology. 6: 181-188.
  11. Cheethaparambil A, Pillai G.S. and Balachandran I. (2014). In vitro microrhizome and minirhizome production in turmeric (Curcuma longa L.) cultivar Alleppey Supreme and its comparative anatomical and histochemical analysis. International Journal of Current Microbiology and Applied Sciences. 3(3): 535-542. 
  12. Das K and Saikia L.R. (2019). Phenological studies of Curcuma caesia Roxb. and Curcuma aromatica Salisb. of Zingiberaceae from upper Brahmaputra Valley, Assam, India. International Journal of scientific and Technology Research. 8(7): 607-609.
  13. Dixit D, Srivastava NK (2000). Distribution of photosynthetically fixed 14-CO2 into curcumin and essential oil in relation to primary metabolites in developing turmeric (Curcuma longa) leaves. Plant Sci. 152: 165-71.
  14. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 11-15.
  15. Duncan, D.B. (1955). Multiple range and multiple F-tests. Biometrics. 11: 1-42.
  16. Isah T (2019). De novo in vitro shoot morphogenesis from shoot tip-induced callus cultures of Gymnema sylvestre (Retz.) R.Br. ex Sm. Biol Res. 52(3).
  17. Islam, M.A.; Kloppstech, K and Jacobsen, H.J. (2004). Efficient Procedure for In vitro Microrhizome Induction in Curcuma longa (Zingiberaceae) - A Medicinal Plant of Tropical Asia. Plant Tissue Cult. 14(2): 123-134.
  18. Kambaska, K.B. and Santilata, S. (2009). Effect of plant growth regulator on micropropagtion of ginger (Zingiber officinale Rosc.) cv- Suprava and Suruchi. Journal of Agricultural Technology. 5(2): 271-280.
  19. Khatun, A., Nasrin, S. and Hossain, M.T. (2003). Large Scale Multiplication of Ginger (Zingiber officinale Rosc.) from Shoot-tip Culture. Journal of Biological Sciences. 3(1): 59-64.
  20. Khumaida N., Ardie S.W., Setiadi A., Artiningsih L.N. (2019). In vitro multiplication and acclimatization of black galingale (Curcuma Aeruginosa Roxb.) Journal of Applied Pharmaceutical Science. 9(4): 110-116. 
  21. Mannangatti, K. and Narayanasamy, M. (2008). Anti-fungal protein from Curcuma caesia Roxb. J. Biotechnol. S136–90.
  22. Murashige T. and Skoog F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473-479.
  23. Nayak S. (2000). In vitro multiplication and microrhizome induction in Curcuma aromatica Salisb. Journal Plant Growth Regulation. 32(1): 41-47.
  24. Panda M., Mohanty S. Subudhi E., Acharya L., Nayak S. (2007). Assessment of stability of micropropagated plants of Curcuma longa L., by cytophotometry and RAPD analyses, International Journal of Integrative Biology. 1(3): 189-195.
  25. Parthasarathy, V.A. and Sasikumar, B. (2006). Biotechnology of Curcuma, CAB Reviews: Perspectives in Agric, Vet. Sci., Nut. and Nat. Resources. 1(20): 1-9.
  26. Shahinozzaman M., Muhammad F.M., Muhammad O.F., Azad M.A.K. and Amin, M.N. (2013). Micropropagation of black turmeric (Curcuma caesia Roxb.) through in vitro culture of rhizome bud explants. Journal of Central European Agriculture. 14(3): 110-115.
  27. Sharma T.R. and Singh B.M. (1995). In vitro microrhizome production in Zingiber officinale Rosc. Plant Cell Rep. 15: 274-277.
  28. Sasikumar B. (2005). Genetic resource of Curcuma: diversity, characterization and utilization. Plant Genet Resource. 3: 230-251.
  29. Shirgurkar M.V., John C.K., Nadgauda R.S. (2001). Factors affecting in vitro micro-rhizome production in turmeric. Plant Cell, Tissue and Organ Cult. 64(1): 5-11.
  30. Stanly C. and Keng C.L. (2007). Micropropagation of Curcuma zedoaria Rosc. and Zingiber zerumbet Smith. Biotechnology. 6(4): 555-560
  31. Sunitibala H., Damayanti M. Sharma G.J. (2001). In vitro propagation and rhizome formation in Curcuma longa Linn. Cytobios. 105(409): 71-82.
  32. Thingujam V and Nayak S (2019). Dynamics of Curcumin content in Curcuma caesia Roxb. rhizomes with different storage conditions and durations. International Journal of Chemical Studies. 7(3): 1733-1735.
  33. Williams J.G.K. et al. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuclic Acid Research. 18: 65.
  34. Yasuda K., Tsuda T., Shimizu H. and Sugaya A. (1987). Multiplication of Curcuma species by tissue culture. Plant. Med. 54: 75-78.
  35. Zapata E.V., Morales G.S., Lauzardo A.N.H., Bonfil B.M., Tapia G.T., Sánchez A.D.J., Valle M.V.D., Aparicio A.J. (2003). In vitro regeneration and acclimatization of plants of Turmeric (Curcuma longa L.) in a hydroponic system. Biotecnología Aplicada. 20: 25-31.

Editorial Board

View all (0)