Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil

DOI: 10.18805/IJARe.A-5328    | Article Id: A-5328 | Page : 87-92
Citation :- Phosphate Solubilization and Plant Growth Promoting Actinobacteria from Rhizosphere Soil.Indian Journal Of Agricultural Research.2021.(55):87-92
K. Balakrishnan, J. Thirumalairaj, M. Radhakrishnan, V. Gopikrishnan, R. Balagurunathan balanaturelife@gmail.com
Address : Department of Microbiology, Actinobacterial Research Laboratory, Periyar University, Salem-636 011, Tamil Nadu, India.
Submitted Date : 1-07-2019
Accepted Date : 20-04-2020

Abstract

Background: Phosphorus has been considered as the key element as it is directly involved in most of the life processes including in plants. Soil contains both organic and inorganic forms of phosphorus. Phosphate solubilization is the important property of soil bacteria to develop them as plant growth promoting bacteria in the agricultural field. The present study report the phosphate solubilizing and plant growth promoting properties of selected actinobacteria isolated from rhizosphere soil.
Methods: Actinobacterial strains were isolated from rhizosphere soil and screened for in vitro phosphate solubilizing properties using agar plate method. The efficiency of phosphate solubilization and phosphatase activity of isolated actinobacterial strains were tested using Pikovskaya broth. 
Result: In the present study phosphate solubilization and phosphatase activity of isolated actinobacteria, Streptomyces sp. CTD-2 was comparatively higher in lab conditions. In pot trial experiment strain CTD-2 showed higher growth when compared to the control plant. The chlorophyll content of leaves in the experimental plants were found maximum with actinobacteria strain CTD-2 treated plant. Plant growth measurements such as root length, shoot length, leaf length, total plant growth measurements, quick yield production were also observed. 

Keywords

Actinobacteria Biodiversity Biofertilizer Plant growth Phosphate Streptomyces

References

  1. Arnon, DI. (1949). Copper enzymes in isolated chloroplasts. Polyphenol oxidase in Beta vulgaris. Plant Physiology. (24): 1-15. 
  2. Artidaue and Patel, RH. (1999). Inorganic phosphate solubilizing soil Pseudomonas. Indian Journal of Microbiology. 39: 161-164.
  3. Azziz, G., Bajsa, N., Haghjou, T., Taulé, C., Valverde, Á., Igual, J. M. and Arias, A. (2012). Abundance, diversity and prospecting of culturable phosphate solubilizing bacteria on soils under crop-pasture rotations in a no-tillage regime in Uruguay. Applied Soil Ecology. 61: 320-326.
  4. Babalola, O.O. and Glick, B.R. (2012). Indigenous African agriculture and plant associated microbes: current practice and future transgenic prospects. Sci. Res. Essays. 28: 2431-2439.
  5. Bekhit, SR., Hassan, AH., Ramadan, IVIH., AlAnany, AMA. (2005). Effect of different levels and sources of nitrogen on growth, yield and quality of potatoes grown under sandy soil conditions. Annals of Agricultural Science, Moshtohor Journal. 43: 381-394.
  6. Bhattacharyya, P.N. and Jha, D.K. (2012). Plant growth-promoting rhizobacteria (PGPR): emergence in agriculture. World Journal of Microbiology and Biotechnology. 28: 1327-1350.
  7. Dadarwal, L.R.L.S., Yadav and S.S., simdhu, (1997). Biofertilizer production technology prospects. In Bio Technological Approaches in Soil Microorganisms for Sustainable Crop Production. Scientific Publishers, Jodhpur, India. 323-337.
  8. Eileen Ingham, K.T., Holland, G., Gowland and W.J., Cunliffe. (1979). Purification and partial characterization of an acid phosphatase produced by Propionibacterium acnes. Journal of General Microbiology. 118: 59-65.
  9. Hardy, J.E., Karamushka, V.I., Gruzina, T.G., Nikovska, G.N., Sayer, J.A. and Gadd, G.M. (1998). Influence of the carbon, nitrogen and phosphorus source on the solubilization of insoluble metal compounds by Aspergillus niger. Mycological Research. 102: 1050-1054.
  10. Kumar, G.P., Desai, S., Amalraj, E.L.D. and Pinisetty, S. (2015). Impact of seed bacterization with PGPR on growth and nutrient uptake in different cultivable varieties of green gram. Asian Journal of Agricultural Research. 9: 113-122.
  11. Manulis Shulamit, Kogan Nina, Valinsky Lea, Levi Michal, Wieman Liora, (1996). Detection of Plant Pathogenic Bacteria in Ornamentals by the PCR Technique. Diagnosis and Identification of Plant Pathogens, Volume. 11:189-192.
  12. Manulis, S., Shafrir, H., Epstein, E., Lichter, A. and Barash, I. (1994). Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp. Microbiology. 140: 1045-1050.
  13. Parth Vinodrai Bhatt and Bharatkumar Rajiv Manuel Vyas, (2014). Screening and characterization of plant growth and health promoting rhizobacteria. Int. J. Curr. Microbiol. App. Sci. 6: 139-155.
  14. Pathom-Aree, W., Stach, J.E., Ward, A.C., Horikoshi, K., Bull, A.T. and Goodfellow, M. (2006). Diversity of actinomycetes isolated from Challenger Deep sediment from the Mariana Trench. Extremophiles. 10: 181-189.
  15. Pedregosa, A.M., Pinto, F., Monistrol, I.F., Laborda. F, (1991). Regulation of acid and alkaline phosphatases of Cladosporium cucumerinum by inorganic phosphate. Mycological Research. 95: 720-724.
  16. Pikovskaya, R.I, (1948). Mobilization of phosphorus in soil in connection with vital activities by some microbial species. Microbiologia. 17: 362-370.
  17. Pisano, M.A., Sommer, M.J. and Lopez, M.M. (1986). Application of pretreatments for the isolation of bioactive actinomycetes from marine sediments. Applied microbiology and biotechnology. 25: 285-288.
  18. Puente, M. E., Bashan, Y., Li, C.Y., Lebsky, V. K, (2004). Microbial populations and activities in the rhizosphere of rock-weathering desert plants. Root colonisation and weathering of igneous rocks. Plant Biology. 6: 629-642. 
  19. Saif, Saima, et al., (2014). Role of phosphate-solubilizing Actinomycetes in plant growth promotion: current perspective. Phosphate Solubilizing Microorganisms. Springer, Cham. 137-156.
  20. Salem MA, AL-ZayadnehW, Abdul-Jaleel C. (2010). Effects of compost interactions on the alterations in mineral biochemistry, growth, tuber quality and production of Solanum tuberosum. Frontiers of Agriculture in China. 4: 170-174. 
  21. Shirting, E.B. and Gottlieb, D, (1966). Methods for characterization o Streptomyces species. International Journal of Syst Bacteriol. 16: 313-340.
  22. Sudhansupal, (1999). Selection of acid tolerant strains of phosphate solubilizing bacteria in soil of Uttar Pradesh, Himalayas. Indian Journal of Agricultural Science. 69: 787-82.
  23. Vurukonda, S.S.K.P., Giovanardi, D. and Stefani, E. (2018). Plant growth promoting and biocontrol activity of Streptomyces spp. as endophytes. International Journal of Molecular Sciences. 19(4): 952.
  24. Yang, Y.J., Yu, F.L., Chen, L., Zeng, J. M., Yang, S.J., Li, S.F. and Wang, H.S. (2003). Elite germplasm evaluation and genetic stability of tea plants. J Tea Sci. 23: 1-8.
  25. Zhu, F., Qu, L., Hong, X. and Sun, X. (2011). Isolation and characterization of a phosphate-solubilizing halophilic bacterium Kushneria sp. YCWA18 from Daqiao Saltern on the coast of Yellow Sea of China. Evidence-Based Complementary and Alternative Medicine, 2011.

Global Footprints