Comparative investigation of DNA extraction methods in black gram (Vigna mungo (L.) 

DOI: 10.18805/IJARe.A-5212    | Article Id: A-5212 | Page : 749-752
Citation :- Comparative investigation of DNA extraction methods in black gram (Vigna mungo (L.).Indian Journal Of Agricultural Research.2019.(53):749-752
M. Prakash, B. Priyadharshini, M. Vignesh and R. Anandan geeth_prakash@yahoo.co.in
Address : Department of Genetics and Plant Breeding, Faculty of Agriculture, Annamalai University, Annamalai Nagar-608 002, Tamil nadu, India.
Submitted Date : 23-01-2019
Accepted Date : 21-05-2019

Abstract

Isolation of intact, double stranded, pure and non- contaminated genomic DNA is prerequisite for large scale genotyping analysis including DNA-banks. Three methods of DNA isolation (Dellaporta, CTAB and Hi-PurAg DNA isolation kits) from 25 black gram genotypes were compared in terms of the yield, purity, integrity, and stability of extracted DNA. Purity and quantification of isolated DNA samples was confirmed by using the UV nano-spectrophotometer at OD260/280 and the same is confirmed based by agarose gel electrophoresis. The CTAB method showed the best results followed by Hi-PurAg and Dellaporta method. The CTAB DNA extraction method was found to be the most efficient DNA extraction method, capable of providing high quality, pure and stable DNA and could be used for various molecular related works. All the 25 black gram genotypes for this research gave good yield of DNA from the established modified CTAB protocol. 

Keywords

CTAB Gel electrophoresis Isolation UV gel documentation UV nano-spectrophotometer

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