Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Agricultural Research, volume 54 issue 1 (february 2020) : 95-100

Growth and Biochemical Variations in Macrotyloma Uniflorum Var. Madhu Under Chromium Stress

Shilpee Dhali, Madhusmita Pradhan, Ranjan Kumar Sahoo, Chinmay Pradhan, Santanu Mohanty
1Department of Botany, Utkal University, Bhubaneswar-751 003, Odisha, India.
Cite article:- Dhali Shilpee, Pradhan Madhusmita, Sahoo Kumar Ranjan, Pradhan Chinmay, Mohanty Santanu (2019). Growth and Biochemical Variations in Macrotyloma Uniflorum Var. Madhu Under Chromium Stress. Indian Journal of Agricultural Research. 54(1): 95-100. doi: 10.18805/IJARe.A-5209.
The discharge of contaminated mining and industrial residues has become a major cause of environmental pollution. Seed is highly protective against external metal stresses. But they are highly sensitive during vegetative developmental process. In this study, germination was conducted in Macrotyloma uniflorum var. Madhu, in order to find out the effect of chromium toxicity on germination, growth and biochemical variations. The seeds were germinated in five different concentrations of chromium (25ìM, 50ìM, 100ìM, 150ìM and 200 ìM) solution. The hydroponically grown plants revealed the toxic phenotypic expression (root length, shoot length, seedling length, shoot vigor index, shoot fresh weight, shoot dry weight, root fresh weight and root dry weight) of plant after 3 growth stage (15 d, 30 d and 45 d). Similarly biochemical characteristics as well as antioxidant activities (Chlorophyll, carotenoid, carbohydrate, reducing sugar, catalase, proline content and peroxidase) were studied to see the toxic effect of chromium. 
  1. Abdul-Baki, A. A. and Anderson, J. D. (1973). Relationship between decarboxylation of glutamic acid and vigour in soyabean seed. Crop Science. 13: 222-226.
  2. Arun, K., Shanker, T., Cervantes, T., Loza-Tavera, H., Avudainayagam, S. (2005). Chromium toxicity in plants. Environment International. 31: 739-753.
  3. Atodariya, U., Barad, R., Upadhyay, S., Upadhyay, U. (2013). Anti-Urolithiatic activity of Dolichos biflorus Seeds. Journal of Pharmacognosy and Phytochemistry. 2(2): 209-213.
  4. Barcelo, J., Poschenrieder, C., Gunse, B. (1986). Water relations of chromium VI treated bush bean plants (Phaseolus vulgaris L. cv Contender) under both normal and water stress conditions. Journal of Experimental Botany. 37: 178-187.
  5. Bates, L.S., Waldran, R.P., Teare, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil. 39:205-208. 
  6. Krouma, A. and Ab delly, C. (2003). Importance of iron use-efficiency of nodules in common bean (Phaseolus vulgaris) for iron deficiency chlorosis resistance. Journal of Plant Nutrition and Soil Science. 166: 525-528.
  7. Clijsters, H. and Van Assche, F. (1985). Inhibition of photosynthesis by heavy metals. Photosynthesis Research. 7: 31-40.
  8. Epstein, E. (1972). Mineral Nnutrition of Plants: Principles and Perspectives. John Wiley and Sons, London, pp, 412.
  9. Pantola, S., Vibhuti, Kiran, B., Bargali, S.S. (2017). Effects of NaCl on germination and seedling growth in Macrotyloma uniflorum and Vigna mungo. Current Agricultural Research Journal. 5(2): 169-176.
  10. Jarup, L. (2003). Hazards of heavy metals contamination. British Medical Bulletin, 68: 167-182.
  11. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the Folin phenol reagent. The Journal of Biological Chemistry. 193: 265-275.
  12. Mathan, J., Mohanty, M., Pradhan, C., Patra, H. K. (2014). Toxicological changes in rice under nickel stress. Biolife Journal. 2(1): 363-369.
  13. Mohanty. M. and Patra, H.K. (2012). Effect of chelate assisted Hexavalent Chromium on physiological changes, biochemical alterations and Cr bioavailability in crop plants - An in vitro phytoremediation approach. Bioremediation Journal. 16(3): 147-155.
  14. Mohanty, M., Pattanaik, M.M., Misra, A.K., Patra, H.K. (2011). Chromium bioaccumulation in rice grown in contaminated soil and irrigated mine waste water - A case study at south Kaliapani Chromite Mine Area, Orissa, India. International Journal of Phytoremediation. 13: 397-409.
  15. Reddy, A.M., Kumar, S.G., Jyothsnakumari, G., Timmanaik, S., Sudhaka, C. (2005). Lead induced changes in antioxidant metabolism of horse gram [Macrotyloma uniforum (Lam.) Verdc.] and Bengal gram (Cicer arietinum L.). Chemosphere. 60: 97–104.
  16. Panda, S.K. and Patra, H.K. (2000). Nitrate and ammonium ions effect on the chromium toxicity in developing wheat seedlings. Proceedings of the National Academy of Sciences, India Section B. 70: 75-80.
  17. Peralta, J.R., Gardea Torresdey, J.L., Tiemann, K.J., Gomez, E., Arteaga, S., Rascon, E. (2001). Uptake and effects of five heavy metals on seed germination and plant growth in alfalfa (Medicago sativa) L. Bulletin of Environmental Contamination and Toxicology. 66(6): 727-734.
  18. Porra, R.J. (2002). The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynthesis Research. 73(1-3): 149-156.
  19. Prasad, M.N.V. (1998). Metal-biomolecule complexes in plants: occurrence, functions, and applications. Analusis. 26-28.
  20. Sadasivam, S. and Manickam, A. (2008). Biochemical Methods. New Age International Publishers, 3rd ed. New Delhi, India.
  21. Shanker, A.K., Sudhagar, R., Pathmanabhan, G. (2003). Growth, phytochelatin SH and antioxidative response of sunflower as affected by chromium speciation. 2nd International Congress of Plant Physiology on sustainable plant productivity under changing environment, New Delhi, India.
  22. Taiz, L. and Zeiger, E. (2002). Plant Physiology. 3rd ed. Sinauer Associates, Sunderland, M.A. pp. 690. 
  23. Zeid, I.M. (2001). Responses of Phaseolus vulgaris to chromium and cobalt treatments. Biologia Plantarum. 44: 111-115. 

Editorial Board

View all (0)