Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential

DOI: 10.18805/IJARe.A-515    | Article Id: A-515 | Page : 361-366
Citation :- Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential.Indian Journal Of Agricultural Research.2020.(54):361-366
Mizna, Moon Sajid, Sameera Hassan, Bushra Rashid, Tayyab Husnain bushra.cemb@pu.edu.pk
Address : Centre of Excellence in Molecular Biology, University of the Punjab, 87-W Canal Bank Road Thokar Niaz Baig, Lahore-53700, Pakistan.
Submitted Date : 10-12-2019
Accepted Date : 16-02-2020

Abstract

Plant tissue culture technique was used to develop a regeneration procedure in local Pakistani cotton varieties. Three cotton varieties Coker 312, CEMB66 and Klean cotton were cultured on MS media supplemented with vitamins and growth regulators. The results showed that the regeneration potential of Coker 312 was higher, CEMB 66 moderate while Klean cotton lacks the cotton regeneration potential. The molecular analysis identified the Somatic Embryogenesis Receptor Kinase3 (SERK3) gene and its expression analysis confirmed that it plays role for embryo formation through tissue culture. Its low expression resulted in calli to die rather than forming embryos. Therefore, SERK3 gene is considered important for callus induction and its development into primary embryogenic and embryogenic stage. Further investigations will be helpful to understand the mechanism of regeneration potential of cotton through tissue culture.

Keywords

Callus induction Cotton regeneration Embryogenesis Gene expression Plant tissue culture

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