Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 54 issue 3 (june 2020) : 361-366

Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential

Mizna, Moon Sajid, Sameera Hassan, Bushra Rashid, Tayyab Husnain
1Centre of Excellence in Molecular Biology, University of the Punjab, 87-W Canal Bank Road Thokar Niaz Baig, Lahore-53700, Pakistan.
Cite article:- Mizna, Sajid Moon, Hassan Sameera, Rashid Bushra, Husnain Tayyab (2020). Embryogenic Capabilities of Cotton through Tissue Culture and Expression Analysis of SERK3 for Regeneration Potential. Indian Journal of Agricultural Research. 54(3): 361-366. doi: 10.18805/IJARe.A-515.

ABSTRACT

Plant tissue culture technique was used to develop a regeneration procedure in local Pakistani cotton varieties. Three cotton varieties Coker 312, CEMB66 and Klean cotton were cultured on MS media supplemented with vitamins and growth regulators. The results showed that the regeneration potential of Coker 312 was higher, CEMB 66 moderate while Klean cotton lacks the cotton regeneration potential. The molecular analysis identified the Somatic Embryogenesis Receptor Kinase3 (SERK3) gene and its expression analysis confirmed that it plays role for embryo formation through tissue culture. Its low expression resulted in calli to die rather than forming embryos. Therefore, SERK3 gene is considered important for callus induction and its development into primary embryogenic and embryogenic stage. Further investigations will be helpful to understand the mechanism of regeneration potential of cotton through tissue culture.

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