Fungitoxic effect of inorganic salts for the management of seed borne Macrophomina phaseolina and Fusarium sp. causing charcoal rot and wilt disease in blackgram 

DOI: 10.18805/IJARe.A-5146    | Article Id: A-5146 | Page : 208-212
Citation :- Fungitoxic effect of inorganic salts for the management of seed borne Macrophomina phaseolina and Fusarium sp. causing charcoal rot and wilt disease in blackgram.Indian Journal Of Agricultural Research.2019.(53):208-212
N. Indra, A.S. Kauvyashree, D. Swetha, M. Asmina and Shalini nindra73@yahoo.com
Address : Seed Centre, Tamil Nadu Agricultural University, Coimbatore-641 003, Tamil Nadu, India.
Submitted Date : 5-10-2018
Accepted Date : 2-02-2019

Abstract

The laboratory experiments were carried out to study the effect of different salts viz., potassium chloride (KCl), potassium phosphate dibasic (K2HPO4), sodium carbonate (Na2CO3), sodium bicarbonate (NaHCO3) and ammonium molybdate (NH4)6 Mo7O24) on seed borne M. phaseolina and Fusarium sp. as an alternative to synthetic fungicides for the control of charcoal rot and wilt diseases in blackgram. The evaluation of different salts was performed in vitro using various concentrations viz., 0.5, 1.0, 2.0, 4.0 and 8.0 per cent (w/v). Among the salts tested against M. phaseolina, sodium carbonate, sodium bicarbonate and ammonium molybdate at 0.5, 2.0 and 4.0 per cent respectively inhibited the fungal growth. Among the salts tested against Fusarium sp. sodium carbonate (Na2CO3) at 4.0 per cent concentration recorded complete inhibition of the mycelial growth compared to the other salts. Also seed priming of these salts significantly reduced the seed borne infection due to M. phaseolina and Fusarium sp. under standard blotter test. The salts like potassium chloride and potassium phosphate dibasic (K2HPO4) at all concentrations did not inhibit M. phaseolina and Fusarium sp. which recorded 100 per cent mycelial growth as that of control.

Keywords

Ammonium molybdate Blackgram Fusarium Macrophomina phaseolina.

References

  1. Annual Report. (2017-18). Department of Agriculture, Co operation and Farmers Welfare. Ministry of Agriculture and Farmers Welfare. pp.3.
  2. Agarwal, V.K.(1981). Seed borne fungi and viruses of some important crops. Research Bulletin 108, G.B. Pant University of Agri and Tech. Patnagar.
  3. Amir, H. and Mair,A.(1996). Role de la microflore dans la resistance a la Fusariose Vasularie induite par la salinite dans unsol de. Palmarie. Soil. Boil. Biochem., 28(1) : 113-122.
  4. Barnett, H.L. and Hunter,B.B. (1998). Illustrated Genera of Imperfect Fungi. Minesotta: Burgees Pub., Co., 241 pp.
  5. El-Mougy, N.S. and Abdel-Kader,M.M. (2009). Seed and soil treatments as integrated controlmeasure against faba bean root rot pathogens. Plant Pathol. Bull.,18:75-87.
  6. Goudarzi, A., Banihashemi, Z.and Monovchehr,M. (2008). Effect of water potential on sclerotial germination and mycelial growth of M. phaseolina.Phytopathol. Mediterr.,47: 107-114.
  7. Hildebrand, E.M. (1938). Techniques for the isolation of single microorganisms. Bot. Rev., 4: 628-658.
  8. ISTA. (1996). International rules of seed testing. Int. Seed Test. Ass.,31:1-152.
  9. Jabnoun- Khiareddine,H., Abdallah, R., El-Mohamedy, R., Abdel-Kareem, F. and Gueddes-Chahed, M. (2016). Comparitive efficacy of potassium salts against soil borne and air borne fungi and their ability to suppress tomato wilt and fruit rots. J. Microb Biochem Technol 8:045-055. 
  10. Jones, E.E., Steward, A. and Whipps,J.M. (2011). Water potential affects Coniothyrium minitans growth, germination and parasitism of Sclerotinia sclerotia.Fungal Biol.,115: 871-881.
  11. Kostandi, S.F. and Soliman,M. F. (1998). The role of calcium in mediating smut disease severity and salt tolerance in corn under chloride and sulphate salinity. J. Phytopathol.,146: 191-195.
  12. Kuc, J. (1990). Immunization for the control of plant disease. In: Biological Control of Soil Borne Pathogens, [Hornbg, D. (ed.)]. pp. 355-373.
  13. Masoud Zaker, (2014). Antifungal evaluation of some inorganic salts against three phytopathogenic fungi. International Journal of Agriculture and Crop Sciences. 7-14: 1352-1358. 
  14. Mcquilken, M.P.,. Whipps, J.M and Cooke,R.C. (1992). Effects of osmotic and matric potential on growth and oospore germination of the biocontrol agent Pythium oligandrum. Mycol. Res., 96(7): 588-591.
  15. Meacteau, M.R., Arul, J.and Tweddell,R.J. (2002). Effect of organic and inorganic salts on the growth and development of Fusarium sambicinum, a causal agent of potato dry rot. Mycological Research, 106: 688-696.
  16. Mirostera,O.,Siegrist, J.and. Buchneauer,H. (2002). Mechanisms of phosphate induced disease resistance in cucumber. European. J. Plant Pathol., 108:345-353.
  17. Muharrem Turkkam and Ismail Erper. (2014). Evaluation of antifungal activity of sodium salts against onion basal rot caused by Fusarium oxysporum f.sp. cepae. Plant Protect. Sci. 50:1 19-25. 
  18. Neergard, P. (1977). Seed Pathology, Vol 1. The Macmillan press Ltd., London, 835pp.
  19. Neergaard, P. and Saad, A. (1962). Seed health testing of rice, a contribution to development of laboratory routine testing methods. Indian Phytopath., 15 : 85-111.
  20. Olivier C., Halseth,D.E., Mizubuti,E.S.G. and Loria,R. (1998). Postharvest application of organic and inorganic salts for suppression of silver scurf on potato tubers. Plant Disease,82: 213-217.
  21. Pham van Du., Nguyen Be Sau, Tran Ngoe Bich and Pham van Kim. (2001).Induced resistance of rice plant to blast (Pyricularia grisea) by seed treatment using natri – tetraborate (Na2 B4 O7) under field conditions. Omonrice, 9: 96-101. 
  22. Pun, K.B., Sabitha, D., Valluvaparidasan V. and Doraiswamy,S. (1998). Studies on seed borne nature of Macrophomina phaseolina in okra. Plant Dis. Res., 13(2): 162-164. 
  23. Turkkan, M. (2013). Antifungal effect of various salts against Fusarium oxysporum f. sp. cepae, the casual agent of Fusariumbasal rot of onion. Journal of Agricultural Sciences, 91: 178-187. 

Global Footprints