Salt tolerant polyembryonic mango rootstock (ML-2 and GPL-1): A putative role of endophytic bacteria by using BOX-PCR

ABSTRACT

This study was conducted to assess the movement of Na+ and K+ between leaves, stem and root segments of tolerant and susceptible polyembryonic mango accessions under saline-sodic environment. Among the 15 diverse polyembryonic from different location of South Andaman used for screening under saline-sodic condition with the soils of pH 9.45 under pot culture experiments in polyhouse, at the time of completion of experiment (240th DAS) the accessions GPL-1, ML-2 and ML-6 were selected which exhibited tolerance to sodicity with lower mortality percent of 6.67 to 13.33% and the rest of polyembryonic seedling were having huge mortality percent ranging from 96.67 to 100.00%. The Na+/K+ ratio in roots of tolerant accessions registered lower Na+/K+ ratio ranging from 0.47 (GPL-1) to 0.61 (ML-6) and susceptible accessions was higher Na+/K+ which ranged from 3.06 (GTP-2 to 6.53 (GPL-4). The same was reverse in the stem and leaves in tolerant and susceptible accessions. Results indicated that the isolates belonged to four major phylogenetic group: low G+C Gram positive bacteria, Firmicutes, Proteobacteria and Bacteroidetes.

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Ahlawat, S. P. S. (2001). Biodiversity of Andaman and Nicobar Islands. Journal of Andaman Science Association, 17: 1-2.
Brick, J.M., Bostock, R.M. and Silversone, S.E. (1991). Rapid In-situ assay for indole acetic acid production by bacteria immobilized on nitrocellulose membrane. Appl. Environ. Microbiol., 57: 535-538.
Cyrus, H. Fiske and Yellapragada Subbarow (1925). The colorimetric determination of phosphorus. J. Biol. Chem., 66: 375.
Damodaran, T., Kannan, R., Israr Ahmed, Srivastava, R.C., Rai, R.B. and Umamaheshwari, S. (2012). Assessing genetic relationships among mango (Mangifera indica L.) accessions of Andaman Islands using inter simple sequence repeat markers. New Zealand J. Crop Hort. Sc., 1175-8783: 1-12.
Damodaran, T., Medhi, R. P., Singh, D.R., Rai, R.B., Damodaran, V. and Kapil Dev. (2007). Identification of molecular markers linked with differential flowering behaviour of mangoes in Andaman and Nicobar islands. Curr. Sci., 92: 1053-1056.
Damodaran, T., Rai. R.B., Jha, S.K., Sharma, D.K., Mishra, V.K., Dhama, K. and Singh, A.K., Sah, V. (2013). Impact of social factors in adoption of CSR BIO – A cost effective, ecofriently bio-growth enhancer for sustainable crop production. South Asian Journal of Experimental Biology, 3(4): 158-165.
Damodaran, T., Shailendra Rajan, Ram Kumar, Sharma, D. K., Misra, V. K., Jha S. K. and Rai, R. B. (2013). Post-tsunami collection of polyembryonic mango diversity from Andaman Islands and their ex-situ reaction to high sodium in sodic soil. Journal of Applied Horticulture, 15(1): 21-25.
Duffy, B. K. (1999). Environmental factors modulating antibiotic and siderophore biosynthesis by Pseudomonas fluorescens biocontrol strains. Appl. Environ. Microbiol, pp. 2429-438.
El-Banana, N. and Winkelmann, G. (1988). Pyrrolnitrin from Burkholderia cepacia: antibiotic activity against fungi and novel activities against streptomycetes. J Appl Microbiol, 85: 69-78.
Glick, B. R. (1995). The enhancement of plant growth by free living bacteria. Can. J. Microbiol, 41: 109-114.
Gupta, A., Saxena, A.K., Gopal, M. and Tilak K.V.B.R. (1998). Effect of plant growth promoting rhizobacteria on competitive ability of introduced Bradyrhizobium sp. (Vigna) for nodulation. Microbiol Res., 153: 113-117.
Hofte, M., Boelens, J. and Verstraete, W. (1992). Survival and root colonization of mutants of plant growth promoting Pseudomonads affected in siderophore biosynthesis or regulation of siderophore production. J. Plant Nutr., 15: 2253-2262.
Hoult, M. D., Donnelly, M. M. and Smith, M.W. (1997). Salt exclusion varies amongst polyembryonic mango cultivar seedlings. Acta Hort., 455: 455-458.
Hung, P. Q. and Annapurna, K. (2004). Isolation and characterization of endophytic bacteria in soybean (Glycine sp.). Omon Rice., 12: 92-101.
Idriss, E. E. S, Makarewicz, O and Farouk A (2002). Extracellular phytase activity of Bacillus amyloliquefaciens FZB 45 contributes to its plant growth-promoting effect. Microbiology 148:2097–2109.
Janda, J. M. (1991). Recent advances in the study of the taxonomy, pathogenicity and infectious syndromes associated with the genus Aeromonas. Clin Microbiol Rev., 4: 397-410.
Jannathul Firdous and Bhore Subhash, J. (2017). Screening of cultivable endophytic bacterial isolates for their plant growth promoting activity in rice. Indian J. Agric. Res., 51(5): 413-418.
Kannan, R., Damodaran, T., Pandey, B. K., Rai, R. B., Umamaheshwari, S., Sharma, D. K., Mishra, V. K., Jha, S. K. and Sah, V. (2014). Isolation and characterization of endophytic plant growth-promoting bacteria (PGPB) associated to the sodicity tolerant polyembryonic mango (Mangifera indica L.) root stock and growth vigour in rice under sodic environment. African Journal of Microbiology Research, 8 (7): 628-636.
Naik, P. R., Raman, G., Narayanan, K. B. and Sakthivel, N. (2008). Assessment of genetic and functional diversity of phosphate solubilizing fluorescent pseudomonads isolated from rhizospheric soil. BMC Microbiol., 8: 230.
Rademaker, J. L.W. and De Bruijn, F. J. (1997). Characterization and classification of microbes by REP-PCR genomic fingerprinting and computer-assisted pattern analysis. In: Akkermans ADL, van Elsas JD, de Bruijn FJ, editors. DNA markers: protocols, applications and overviews. New York: J. Wiley and Sons, pp. 1-26.
Raju, N. S., Niranjan, S. R., Janardhan, G. R., Prakash, H. S. and Mathur, S. B. (1999). Improvement of seed quality and field emergence of Fusarium moniliformae infected sorghum seeds biocontrol agents. J. Sci. Food., 79: 206-212.
Samara, J. S. (1985). Effect of irrigation water and soil sodicity on the performance and leaf nutrient composition of mango cultivars. Acta. Hort., 231: 306-311.
Schwyn, B. and Neilands, J. B. (1987). Universal chemical assay for detection and determination of siderophore. Anal. Biochem., 160: 47-56.
Son, H. J., Park, G. T., Cha, M. S. and Heo, M. S. (2006). Solubilization of insoluble inorganic phosphates by a novel salt and pH tolerant Pantoea agglomerans R-42 isolated from soybean rhizosphere. Biores. Technol, 97: 204-210.
Usha, R. M. and Arundhathi, Reddy, G. (2012). Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea. A. J. Biotechnol, 11(32): 8085-8091.
Varu, D. K. and Barad, A. V. (2010). Standardization of mango rootstock for mitigating salt stress. Indian J. Hort., 67: 79-83.

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Salt tolerant polyembryonic mango rootstock (ML-2 and GPL-1): A putative role of endophytic bacteria by using BOX-PCR

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