Indian Journal of Agricultural Research

  • Chief EditorT. Mohapatra

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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  • SJR 0.293

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Indian Journal of Agricultural Research, volume 52 issue 5 (october 2018) : 497-504

Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri 

Prabhat Jatav, Sonu Singh Ahirwar, Abhishek Gupta, Kirti Kushwaha, Sadhna Jatav
1Center for Microbiology and Biotechnology Research and Training Institute, Bhopal-462 003, Madhya Pradesh, India.
Cite article:- Jatav Prabhat, Ahirwar Singh Sonu, Gupta Abhishek, Kushwaha Kirti, Jatav Sadhna (2018). Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri. Indian Journal of Agricultural Research. 52(5): 497-504. doi: 10.18805/IJARe.A-4999.
Citrus canker is a bacterial disease which occurs in citrus (lemon) species produce by Xanthomonas especially X. citri. The infection occurs on the lesions of leaves, stems, and fruits of citrus trees, it also occurs in other plants such as orange, grapes. The main goal of this study was  to prevent the lemon (citrus) disease such as citrus canker by using some cellulase enzymes produces by soil fungi. In the present study, we isolated soil fungi Trichoderma viride, which produces cellulase enzyme these have higher antibacterial activities against plant pathogen such as X. citri. This enzyme produced by T. viride by using fermentation method, quantified by DNAS method and used as a bio-control agent against X. citri. They also isolated and characterized X. citri. In the last step of the study they have checked antibacterial activity of cellulase enzyme against X. citri. The present study concluded that cellulase enzyme produced by T. viride shows higher antibacterial activity against X. citri when treated it at 100°C.
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