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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 5 (october 2018) : 497-504,   Doi: 10.18805/IJARe.A-4999

Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri 

P
Prabhat Jatav
S
Sonu Singh Ahirwar
A
Abhishek Gupta
K
Kirti Kushwaha
S
Sadhna Jatav
1Center for Microbiology and Biotechnology Research and Training Institute, Bhopal-462 003, Madhya Pradesh, India.
Cite article:- Jatav Prabhat, Ahirwar Singh Sonu, Gupta Abhishek, Kushwaha Kirti, Jatav Sadhna (2018). Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri. Indian Journal of Agricultural Research. 52(5): 497-504. doi: 10.18805/IJARe.A-4999.

ABSTRACT

Citrus canker is a bacterial disease which occurs in citrus (lemon) species produce by Xanthomonas especially X. citri. The infection occurs on the lesions of leaves, stems, and fruits of citrus trees, it also occurs in other plants such as orange, grapes. The main goal of this study was  to prevent the lemon (citrus) disease such as citrus canker by using some cellulase enzymes produces by soil fungi. In the present study, we isolated soil fungi Trichoderma viride, which produces cellulase enzyme these have higher antibacterial activities against plant pathogen such as X. citri. This enzyme produced by T. viride by using fermentation method, quantified by DNAS method and used as a bio-control agent against X. citri. They also isolated and characterized X. citri. In the last step of the study they have checked antibacterial activity of cellulase enzyme against X. citri. The present study concluded that cellulase enzyme produced by T. viride shows higher antibacterial activity against X. citri when treated it at 100°C.

REFERENCES

  1. Ahmed R and Khan HU. (1999). Citrus decline problems and progress in the Punjab: A review. University of Agriculture Faisalabad Pakistan, Proc. 2nd Nat. Conf. Plant Pathology, 20-22.
  2. Aneja KR. (2009). Experiment in microbiology plant pathology and biotechnology. Fourth edition, New age international (P) Limited Publishers, India, 108.
  3. Abbas SZ, Hussain K, Hussain Z, Ali R and Abbas T (2016) Anti-Bacterial Activity of Different Soaps Available in Local Market of Rawalpindi (Pakistan) against Daily Encountered Bacteria. Pharm Anal Acta; 7(11): 522.
  4. Bradbury JF. (1970). Isolation and preliminary study of bacteria from plants. PANS Pest Articles and News Summ, 16 (4): 632-637.
  5. Barnett HL and Binder FL. (1973). The fungal host parasite relationship. Annu Rev Phytopathol; 11: 273-292.
  6. Bayer EA, Morag F and Lamed R. (1994). The cellulosomes a treasure trove for biotechnology. Trends in Biotechnol; 12(9): 379-386.
  7. Bayer EA, Chanzy H, Lamed R and Shoham Y. (1998). Cellulose cellulases and cellulosomes. Curr Opin Struct Biol; 8 (5): 548-557. 
  8. Carvalho LMJ, Deliza R, Silva CAB, Miranda RM and Maia MCA. (2003). Identifying the adequate process conditions by consumers for pineapple juice using membrane technology. J Food Technol; 4 (1): 150-156.
  9. Cheng YS, Ko TP, Wu TH, Ma Yanhe, Huang CH, Lai HL, Wang AHJ, Liu JR and Guo RT. (2011). Crystal structure and substrate binding mode of cellulase 12 A from Thermotoga maritime. Prot Struct Funct Bioinformat; 79 (4): 1193-1204.
  10. Durrell LW. (1968) Hyphal invasion by Trichoderma viride. Mycopathol et Mycol Appl; 35(2): 138-144. 
  11. Elad Y, Chet I and Katan Y. (1980). Trichoderma harzianum a biocontrol agent effective against Sclerotium rolfsii and Rhizoctonia solani. Phytopath; 70 (2): 119-121.
  12. Henrissat B, Teeri TT and Warren R. (1998). A scheme for designating enzymes that hydrolyze the polysaccharides in the cell walls of plants. FEBS Letters; 425 (2): 352–354.
  13. Khan MA, Khan MM, Haq MI and Javed N. (1992). Antibacterial activity of various toxicants against Xanthomonas campestris pv citri for the control of citrus canker disease. Proc 1st Int. Sem. Citri culture in Pakistan, 311-314.
  14. Kondo A, Urabe T and Higashitani K. (1994). Bioconversions in an aqueous two-phase system using enzymes immobilized on ultrafine silica particles. J Ferment Bio-eng; 77(6): 700-703.
  15. Kubicek C.P. (1993). From cellulose to cellulase inducers facts and fiction In Suominen P, Reinikainen T, editors. In Proceedings of the 2nd Symposium Trichoderma Reesei Cellulases and Other Hydrolases (TRICEL 93).Foundation for Biotechnical and Industrial Fermentation Research Espoo Finland, (8): 181-188.
  16. Liu D, Li J, Zhao S, Zhang R, Wang M and Miao Y. (2013). Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources. Biotechnol Biofuels; 6 (149): 6-149.
  17. Mrudula S and Murugammal R. (2011). Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. Braz Microbial J; 42 (3): 1119-1127.
  18. Mehrotra RS. (1980). Bacteria and Bacterial Diseases. Tata McGraw Hill Publishing Co. Ltd, New Delhi, Plant Pathology, 638-639.
  19. Park MS, Seo GS, Lee KH and Bae YSH. (2005). Morphological and cultural characteristics of Trichoderma spp. Associated with green mold of Oyster mushroom in Korea. Plant Pathol J, (21): 221-228.
  20. Pereira A, Carazzolle M, Abe V, Oliveira M, Domingues M, Silva J and Benedetti C.( 2014). Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response. BMC Genomics; 15: 157.
  21. Patil KC, Patil AM, Wagh MC and Chandratre S J. (2015). Labscale Production and Purification of Cellulase Enzyme from Aspergillus Niger. Res J Recent Sci; 4 (IYSC-2015): 124-126.
  22. Sang ML and Koo YM. (2001). Pilot scale production of cellulase using Trichoderma reesei Rut C-30 in fed batch mode. J Microbiol and Biotechnol; 11(2): 229-233.
  23. Scortichini M, Marchesi U and Di Prospero P. (2001). Genetic diversity of Xanthomonas arboricola pv Juglandis (synonyms: X. campestris pv. juglandis; X. juglandis pv. juglandis) strains from different geographical areas shown by repetitive polymerase chain reaction genomic fingerprinting. J Phytopathol; 149 (6): 325-332.
  24. Samuels GJ, Chaverri P, Farr DF and Mccray EB (2002). Trichoderma species associated with the green mold epidemic of commercially grown agaricus bisporus. Mycologia, 94 (1): 146-170.
  25. Whiteside J, Garnsey S and Timmer L. (1988). Compendium of Citrus Diseases. APS Press, St. Paul, MN.
  26. Zhang YHP, Himmel ME and Mielenz JR. (2006). Outlook for cellulase improvement screening and selection strategies. Biotechnol Advan; 24(5): 452-481. 
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Indian Journal of Agricultural Research
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    Research Article

    volume 52 issue 5 (october 2018) : 497-504,   Doi: 10.18805/IJARe.A-4999

    Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri 

    P
    Prabhat Jatav
    S
    Sonu Singh Ahirwar
    A
    Abhishek Gupta
    K
    Kirti Kushwaha
    S
    Sadhna Jatav
    1Center for Microbiology and Biotechnology Research and Training Institute, Bhopal-462 003, Madhya Pradesh, India.
    Cite article:- Jatav Prabhat, Ahirwar Singh Sonu, Gupta Abhishek, Kushwaha Kirti, Jatav Sadhna (2018). Antagonistic activity of cellulase enzyme produced by Trichoderma Viride against Xanthomonas Citri. Indian Journal of Agricultural Research. 52(5): 497-504. doi: 10.18805/IJARe.A-4999.

    ABSTRACT

    Citrus canker is a bacterial disease which occurs in citrus (lemon) species produce by Xanthomonas especially X. citri. The infection occurs on the lesions of leaves, stems, and fruits of citrus trees, it also occurs in other plants such as orange, grapes. The main goal of this study was  to prevent the lemon (citrus) disease such as citrus canker by using some cellulase enzymes produces by soil fungi. In the present study, we isolated soil fungi Trichoderma viride, which produces cellulase enzyme these have higher antibacterial activities against plant pathogen such as X. citri. This enzyme produced by T. viride by using fermentation method, quantified by DNAS method and used as a bio-control agent against X. citri. They also isolated and characterized X. citri. In the last step of the study they have checked antibacterial activity of cellulase enzyme against X. citri. The present study concluded that cellulase enzyme produced by T. viride shows higher antibacterial activity against X. citri when treated it at 100°C.

    REFERENCES

    1. Ahmed R and Khan HU. (1999). Citrus decline problems and progress in the Punjab: A review. University of Agriculture Faisalabad Pakistan, Proc. 2nd Nat. Conf. Plant Pathology, 20-22.
    2. Aneja KR. (2009). Experiment in microbiology plant pathology and biotechnology. Fourth edition, New age international (P) Limited Publishers, India, 108.
    3. Abbas SZ, Hussain K, Hussain Z, Ali R and Abbas T (2016) Anti-Bacterial Activity of Different Soaps Available in Local Market of Rawalpindi (Pakistan) against Daily Encountered Bacteria. Pharm Anal Acta; 7(11): 522.
    4. Bradbury JF. (1970). Isolation and preliminary study of bacteria from plants. PANS Pest Articles and News Summ, 16 (4): 632-637.
    5. Barnett HL and Binder FL. (1973). The fungal host parasite relationship. Annu Rev Phytopathol; 11: 273-292.
    6. Bayer EA, Morag F and Lamed R. (1994). The cellulosomes a treasure trove for biotechnology. Trends in Biotechnol; 12(9): 379-386.
    7. Bayer EA, Chanzy H, Lamed R and Shoham Y. (1998). Cellulose cellulases and cellulosomes. Curr Opin Struct Biol; 8 (5): 548-557. 
    8. Carvalho LMJ, Deliza R, Silva CAB, Miranda RM and Maia MCA. (2003). Identifying the adequate process conditions by consumers for pineapple juice using membrane technology. J Food Technol; 4 (1): 150-156.
    9. Cheng YS, Ko TP, Wu TH, Ma Yanhe, Huang CH, Lai HL, Wang AHJ, Liu JR and Guo RT. (2011). Crystal structure and substrate binding mode of cellulase 12 A from Thermotoga maritime. Prot Struct Funct Bioinformat; 79 (4): 1193-1204.
    10. Durrell LW. (1968) Hyphal invasion by Trichoderma viride. Mycopathol et Mycol Appl; 35(2): 138-144. 
    11. Elad Y, Chet I and Katan Y. (1980). Trichoderma harzianum a biocontrol agent effective against Sclerotium rolfsii and Rhizoctonia solani. Phytopath; 70 (2): 119-121.
    12. Henrissat B, Teeri TT and Warren R. (1998). A scheme for designating enzymes that hydrolyze the polysaccharides in the cell walls of plants. FEBS Letters; 425 (2): 352–354.
    13. Khan MA, Khan MM, Haq MI and Javed N. (1992). Antibacterial activity of various toxicants against Xanthomonas campestris pv citri for the control of citrus canker disease. Proc 1st Int. Sem. Citri culture in Pakistan, 311-314.
    14. Kondo A, Urabe T and Higashitani K. (1994). Bioconversions in an aqueous two-phase system using enzymes immobilized on ultrafine silica particles. J Ferment Bio-eng; 77(6): 700-703.
    15. Kubicek C.P. (1993). From cellulose to cellulase inducers facts and fiction In Suominen P, Reinikainen T, editors. In Proceedings of the 2nd Symposium Trichoderma Reesei Cellulases and Other Hydrolases (TRICEL 93).Foundation for Biotechnical and Industrial Fermentation Research Espoo Finland, (8): 181-188.
    16. Liu D, Li J, Zhao S, Zhang R, Wang M and Miao Y. (2013). Secretome diversity and quantitative analysis of cellulolytic Aspergillus fumigatus Z5 in the presence of different carbon sources. Biotechnol Biofuels; 6 (149): 6-149.
    17. Mrudula S and Murugammal R. (2011). Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate. Braz Microbial J; 42 (3): 1119-1127.
    18. Mehrotra RS. (1980). Bacteria and Bacterial Diseases. Tata McGraw Hill Publishing Co. Ltd, New Delhi, Plant Pathology, 638-639.
    19. Park MS, Seo GS, Lee KH and Bae YSH. (2005). Morphological and cultural characteristics of Trichoderma spp. Associated with green mold of Oyster mushroom in Korea. Plant Pathol J, (21): 221-228.
    20. Pereira A, Carazzolle M, Abe V, Oliveira M, Domingues M, Silva J and Benedetti C.( 2014). Identification of putative TAL effector targets of the citrus canker pathogens shows functional convergence underlying disease development and defense response. BMC Genomics; 15: 157.
    21. Patil KC, Patil AM, Wagh MC and Chandratre S J. (2015). Labscale Production and Purification of Cellulase Enzyme from Aspergillus Niger. Res J Recent Sci; 4 (IYSC-2015): 124-126.
    22. Sang ML and Koo YM. (2001). Pilot scale production of cellulase using Trichoderma reesei Rut C-30 in fed batch mode. J Microbiol and Biotechnol; 11(2): 229-233.
    23. Scortichini M, Marchesi U and Di Prospero P. (2001). Genetic diversity of Xanthomonas arboricola pv Juglandis (synonyms: X. campestris pv. juglandis; X. juglandis pv. juglandis) strains from different geographical areas shown by repetitive polymerase chain reaction genomic fingerprinting. J Phytopathol; 149 (6): 325-332.
    24. Samuels GJ, Chaverri P, Farr DF and Mccray EB (2002). Trichoderma species associated with the green mold epidemic of commercially grown agaricus bisporus. Mycologia, 94 (1): 146-170.
    25. Whiteside J, Garnsey S and Timmer L. (1988). Compendium of Citrus Diseases. APS Press, St. Paul, MN.
    26. Zhang YHP, Himmel ME and Mielenz JR. (2006). Outlook for cellulase improvement screening and selection strategies. Biotechnol Advan; 24(5): 452-481. 
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