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Research Article

volume 52 issue 4 (august 2018) : 434-438,   Doi: 10.18805/IJARe.A-4941

ISSR marker based diversity assessment of Piper spp. in Bay Islands, India 

K
K. Abirami
V
V. Baskaran
P
P. Simhachalam
Cite article:- Abirami K., Baskaran V., Simhachalam P. (2018). ISSR marker based diversity assessment of Piper spp. in Bay Islands, India. Indian Journal of Agricultural Research. 52(4): 434-438. doi: 10.18805/IJARe.A-4941.

ABSTRACT

Inter-simple sequence repeat (ISSR) analysis was used to study the genetic diversity of Piper spp.  with its relative genus Peperomia pellucida. Among the 38 ISSR primers used, 10 primers generated 1083 bands, of which seven (80.8%) were polymorphic. The maximum polymorphism were obtained from the primers UBC 881, UBC 889, UBC 848 and UBC 830. Cluster analysis grouped the species studied into two major clusters. One cluster comprised the different accessions of Piper nigrum and is distinct from the other species of Piperaceae. The other cluster grouped the four species of Piperaceae namely Piper longum, Piper sarmentosum, Piper betle and Peperomia pellucida. The variability studies also revealed that the ISSR marker could group the accessions within the same species based on their similar geographical origin. Assessment of genetic diversity among the different species of the family Piperaceae through ISSR marker would help in rapid identification of polymorphism, assist in future germplasm collection, conservation and domestication programmes. 

REFERENCES

  1. Ahmad, I., Bhagat, S., Sharma, T.V.R.S., Kumar, K. and Simachalam, P. (2010). ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andaman. Indian J. Hort., 67(2): 147-151.
  2. Asha, K.I., Nair, G.M. and Nair, M.C. (2006). Inter-relationship among the species of Dioscorea revealed by morphological trait and RAPD marker. Indian J. Plant Genet. Resources. 19: 40-46.
  3. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15. 
  4. Gentry, H. S. (1955). Apomixis in black pepper and jojoba. Heredity. 46: 8-13.
  5. Ghafoor, S., Shah, M.M. and Ahmad, H. (2007). Molecular characterization of Ephedra species found in Pakistan. Genet. Mol. Res. 6: 1123-30.
  6. Gupta, M., Chyi, Y. S., Romero-Severson., Jowen, J.L. (1994). Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor. Appl. Genet. 89: 998- 1006.
  7. Jiang, L. and Liu, J. (2001). Analysis of genetic diversity of Piper spp. in Hainan Island (China) using inter-simple sequence repeat ISSR markers. African J. Biotechnol. 10(66): 14731-14737.
  8. Joy, N., Abraham, Z. and Soniya, E.V. (2007). A preliminary assessment of genetic relationships among agronomically important cultivars of black pepper. BMC Genet. 8: 42.
  9. Kingdom, K., Weston, F.M., Kwapata, M.B., Bokasi, J.M. and ,Munyenyembe, P. (2007). Genetic diversity of Annona selegalensis Pers. population as revealed by SSRs. African J. Biotech. 6: 1239-47.
  10. Mathew, S.P., Mohandas, A. and Nair, G.M. (2004). Piper sarmentosum Roxb. – An addition to the Flora of Andaman Islands. Curr. Sci. 87(2): 141-142.
  11. Pandey, R.P. and Diwakar, P.G. (2008). An integrated checklist flora of Andaman and Nicobar Islands, India. J. Econ Taxon Bot. 32.(2): 403-500.
  12. Pradeepkumar, T., Karihaloo, J. L., Archak S, Baldev, A. (2003). Analysis of genetic diversity in Piper nigrum L. using RAPD markers. Genet. Resour. Crop Evol. 50: 469-475.
  13. Prakash, N., Brown, J. F. and Yue-Haw.(1994). An embryological study of Kava, Piper methysticue. Aus. J. Bot. 42: 231-237.
  14. Ravindran, S., Rao, A. A., Naik, V.G., Tikader, A., Mukherjee, P. and Thangavelu, K. (1997). Distribution and variation in mulberry germplasm. Indian J. Plant Genet. Resour. 10 (2):233-242.
  15. Rohlf, F.J. (1998). NTSYS-PC. Numeric taxonomy and multivariate analysis system. Version 2.00. Exeter Software, Setauket. New York 
  16. Saghai-Maroof, M.A., Soliman, K.M., Jorgesen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA. 81: 8014-8018.
  17. Saji, K. V. (2006). Taxonomic and Genetic Characterization of Black pepper and related Species, Ph. D Thesis, University of Calicut, Kerala, India.
  18. Sasikumar, B., Haridas, P., George, J. K., Saji, K. V., John Zachariah, T. and Ravindran, P. N. (2004). IISR Thevam, IISR Malabar Excel, IISR Girimunda –three new black pepper clones. J. Spices Arom. Crops. 13: 1-5.
  19. Sebastian, A. and Sujatha, V. S. (1996). Isoenzyme variation and species relationship in the genus Piper. J. Tropical Agric. 34: 136-137.
  20. Sharma, A. K. and Bhattacharya, N. K. (1959). Chromosome studies on two genera of family Piperaceae. Genetica. 29: 256-289.
  21. Vinod K., Singh, G., Sharma, R. and Sharma, S.N. (2007). RAPD and protein profiles of cotton varieties. Indian J. Plant Physiol. 12:115-19.
  22. Yang, W., Oliveira, A.C., Godwin., I, Schertz, K. and Bennetzen, J. L. (1996). Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums. Crop Sci. 36:1669-1676.
  23. Zietkiewicz, E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
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    Research Article

    volume 52 issue 4 (august 2018) : 434-438,   Doi: 10.18805/IJARe.A-4941

    ISSR marker based diversity assessment of Piper spp. in Bay Islands, India 

    K
    K. Abirami
    V
    V. Baskaran
    P
    P. Simhachalam
    Cite article:- Abirami K., Baskaran V., Simhachalam P. (2018). ISSR marker based diversity assessment of Piper spp. in Bay Islands, India. Indian Journal of Agricultural Research. 52(4): 434-438. doi: 10.18805/IJARe.A-4941.

    ABSTRACT

    Inter-simple sequence repeat (ISSR) analysis was used to study the genetic diversity of Piper spp.  with its relative genus Peperomia pellucida. Among the 38 ISSR primers used, 10 primers generated 1083 bands, of which seven (80.8%) were polymorphic. The maximum polymorphism were obtained from the primers UBC 881, UBC 889, UBC 848 and UBC 830. Cluster analysis grouped the species studied into two major clusters. One cluster comprised the different accessions of Piper nigrum and is distinct from the other species of Piperaceae. The other cluster grouped the four species of Piperaceae namely Piper longum, Piper sarmentosum, Piper betle and Peperomia pellucida. The variability studies also revealed that the ISSR marker could group the accessions within the same species based on their similar geographical origin. Assessment of genetic diversity among the different species of the family Piperaceae through ISSR marker would help in rapid identification of polymorphism, assist in future germplasm collection, conservation and domestication programmes. 

    REFERENCES

    1. Ahmad, I., Bhagat, S., Sharma, T.V.R.S., Kumar, K. and Simachalam, P. (2010). ISSR and RAPD marker based DNA fingerprinting and diversity assessment of Annona spp. in South Andaman. Indian J. Hort., 67(2): 147-151.
    2. Asha, K.I., Nair, G.M. and Nair, M.C. (2006). Inter-relationship among the species of Dioscorea revealed by morphological trait and RAPD marker. Indian J. Plant Genet. Resources. 19: 40-46.
    3. Doyle, J.J. and Doyle, J.L. (1990). Isolation of plant DNA from fresh tissue. Focus. 12: 13-15. 
    4. Gentry, H. S. (1955). Apomixis in black pepper and jojoba. Heredity. 46: 8-13.
    5. Ghafoor, S., Shah, M.M. and Ahmad, H. (2007). Molecular characterization of Ephedra species found in Pakistan. Genet. Mol. Res. 6: 1123-30.
    6. Gupta, M., Chyi, Y. S., Romero-Severson., Jowen, J.L. (1994). Amplification of DNA markers from evolutionarily diverse genomes using single primers of simple-sequence repeats. Theor. Appl. Genet. 89: 998- 1006.
    7. Jiang, L. and Liu, J. (2001). Analysis of genetic diversity of Piper spp. in Hainan Island (China) using inter-simple sequence repeat ISSR markers. African J. Biotechnol. 10(66): 14731-14737.
    8. Joy, N., Abraham, Z. and Soniya, E.V. (2007). A preliminary assessment of genetic relationships among agronomically important cultivars of black pepper. BMC Genet. 8: 42.
    9. Kingdom, K., Weston, F.M., Kwapata, M.B., Bokasi, J.M. and ,Munyenyembe, P. (2007). Genetic diversity of Annona selegalensis Pers. population as revealed by SSRs. African J. Biotech. 6: 1239-47.
    10. Mathew, S.P., Mohandas, A. and Nair, G.M. (2004). Piper sarmentosum Roxb. – An addition to the Flora of Andaman Islands. Curr. Sci. 87(2): 141-142.
    11. Pandey, R.P. and Diwakar, P.G. (2008). An integrated checklist flora of Andaman and Nicobar Islands, India. J. Econ Taxon Bot. 32.(2): 403-500.
    12. Pradeepkumar, T., Karihaloo, J. L., Archak S, Baldev, A. (2003). Analysis of genetic diversity in Piper nigrum L. using RAPD markers. Genet. Resour. Crop Evol. 50: 469-475.
    13. Prakash, N., Brown, J. F. and Yue-Haw.(1994). An embryological study of Kava, Piper methysticue. Aus. J. Bot. 42: 231-237.
    14. Ravindran, S., Rao, A. A., Naik, V.G., Tikader, A., Mukherjee, P. and Thangavelu, K. (1997). Distribution and variation in mulberry germplasm. Indian J. Plant Genet. Resour. 10 (2):233-242.
    15. Rohlf, F.J. (1998). NTSYS-PC. Numeric taxonomy and multivariate analysis system. Version 2.00. Exeter Software, Setauket. New York 
    16. Saghai-Maroof, M.A., Soliman, K.M., Jorgesen, R.A. and Allard, R.W. (1984). Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci. USA. 81: 8014-8018.
    17. Saji, K. V. (2006). Taxonomic and Genetic Characterization of Black pepper and related Species, Ph. D Thesis, University of Calicut, Kerala, India.
    18. Sasikumar, B., Haridas, P., George, J. K., Saji, K. V., John Zachariah, T. and Ravindran, P. N. (2004). IISR Thevam, IISR Malabar Excel, IISR Girimunda –three new black pepper clones. J. Spices Arom. Crops. 13: 1-5.
    19. Sebastian, A. and Sujatha, V. S. (1996). Isoenzyme variation and species relationship in the genus Piper. J. Tropical Agric. 34: 136-137.
    20. Sharma, A. K. and Bhattacharya, N. K. (1959). Chromosome studies on two genera of family Piperaceae. Genetica. 29: 256-289.
    21. Vinod K., Singh, G., Sharma, R. and Sharma, S.N. (2007). RAPD and protein profiles of cotton varieties. Indian J. Plant Physiol. 12:115-19.
    22. Yang, W., Oliveira, A.C., Godwin., I, Schertz, K. and Bennetzen, J. L. (1996). Comparison of DNA marker technologies in characterizing plant genome diversity: variability in Chinese sorghums. Crop Sci. 36:1669-1676.
    23. Zietkiewicz, E., Rafalski, A. and Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20: 176-183.
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