Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthan using AFLP markers

DOI: 10.18805/IJARe.A-4795    | Article Id: A-4795 | Page : 543-549
Citation :- Analysis of genetic variations in Musa paradisiaca cv Karibale-Monthanusing AFLP markers.Indian Journal Of Agricultural Research.2017.(51):543-549
R. Shashikumar, V. Krishna, Venkatesh and S. Lokesha
Address : Department of Studies and Research in Biotechnology and Bioinformatics, Kuvempu University, Shankaraghatta-577 451, Karnataka, India
Submitted Date : 20-05-2017
Accepted Date : 12-10-2017


In the present study, the genetic variations of leaf calli regenerants of Musa paradisiaca cv Karibale-Monthan has been analyzed using AFLP markers. The highest (86.66%) frequency of compact callus formation was obtained when leaf explants were cultured on MS media, containing 3mg/L 2,4-D and 0.5 mg/L BAP. The optimum shoot formation and multiplication (5.70±1.49) was achieved from the leaf derived callus on MS medium fortified with 3mg/L BAP+0.5mg/L TDZ. The 3-4 cm small shoots produced distinct roots on MS media containing 0.5mg/l NAA with 0.2% activated charcoal. The AFLP analyses produced 1012 monomorphic bands from total 1094 bands with 7.5% polymorphism between mother plant and callus derived regenerants. The percentage of monomorphism for individual primer combinations was very high and varied from 90.3% (EcoRI-AG×MseI-CCG) to 97.6% (EcoRI-AT×MseI-CGA). The result showed lack of polymorphism is due to genetic integrity of the plants that was not altered indifferent to the hormonal treatment.


AFLP Callus Genetic diversity Indirect organogenesis Karibale-Monthan


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