Indian Journal of Agricultural Research

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Research Article

Indian Journal of Agricultural Research, volume 50 issue 3 (june 2016) : 249-253

Characterization and antagonistic effect of isolated Trichoderma sp. against pathogens under Clusterbean (Cyamopsis tetragonoloba L.)

Anand Kumar Meena, Ashok Kumar Meena
1<p>Department of Plant Pathology,&nbsp;S.K.R.A.U., College of Agriculture, Bikaner- 334 006, India</p>
Cite article:- Meena Kumar Anand, Meena Kumar Ashok (2016). Characterization and antagonistic effect of isolated Trichoderma sp. against pathogens under Clusterbean (Cyamopsis tetragonoloba L.) . Indian Journal of Agricultural Research. 50(3): 249-253. doi: 10.18805/ijare.v0iOF.8439.

ABSTRACT

A green house experiment was conducted at Department of Plant Pathology, College of Agriculture, Bikaner, to characterize the eight isolates of Trichoderma  sp. ( TH-8, TH-SOG, TH-HMG, TH-BKN, TH-SIK, TH-DUP, TH-JOB and TH-JAI)  were collected from different locations of clusterbean (Cyamopsis tetragonoloba L.) fields in western Rajasthan and tested for their antagonistic property against soil borne plant pathogens. It was further studied for morphological and physiological characteristics such as color of mycelium, pigmentation of substrate, type, amount of mycelium growth, effect of temperature and pH. It was observed that,  Potato Dextrose Agar (PDA) media, temp range 25-300C and pH 7.0-7.5 were found most effective for optimum growth and sporulation of TH-8. The isolate Trichoderma  harzianum-8 (isolated from SKRAU Bikaner, Rajasthan )  was found best up to 70 per cent of inhibition of mycelial growth of different clusterbean pathogen viz., Macrophomina phaseolina, Fusarium oxysporum, Sclerotinia sclerotiorum.

REFERENCES


  1. Azher M., Aslam Khan, M., Inam-ul-Haq, M., Pervez, M. A. and Ummad- ud-DinUmar. (2009). Usefulness of different culture media for in-vitro evaluation of Trichoderma sp. Against seed-borne fungi of economic importance. Pak. J. Phytopathol. 21: 83-88.

  2. Bandopadhyay, S., Subhendu, J. and Dutta, S. (2003). Effect of different pH and temperature levels on growth and sporulation of Trichoderma. Environment and Ecology. 21: 770-773.

  3. Das, B. C., Roy, S. K. and Boro, L. C. (1997). Mass multiplication of Trichoderma species on different media. Indian. J. Agri. Sci. 10: 95-100.

  4. Elad, Y., Chet, I. and Henis, Y. (1981). A selective medium for improving quantitative isolation of Trichoderma sp. from soil. Phytoparasitica. 9: 59-69.

  5. Gams, W. and Bissett, J. (2002). Morphology and identification of Trichoderma . In: Kubicek, C. P. and Harman, G. E. (eds.). Trichoderma and Gliocladium: Basic biology, Taxonomy and Genetics. pp. 3-31.

  6. Haas, D. and Defago, G. (2005). Biological control of soil-borne pathogens by fluorescent pseudomonads. Nature Rev. Microbiol. 3: 307-319.

  7. Hasanzadeh, M., Mohammadifar, M., Sahebany, N. and Etebarian, H.R. (2012). Effect of cultural condition on biomass production of some nematophagous fungi as biological control agent. Egypt. Acad. J. Biolog. Sci. 5: 115-126.

  8. Prajapati,V.P., Joshi, D. M., Gajre, N. K. and Kansara, S. S. (2012). Antagonism of some well-known bioagents against curvularia eragrostidis (henn.) J.a.mey. - an incitant of Spider lilly leaf tip blight. The Bioscan.7: 701-703. 

  9. Raut, I., Constantin, M. Vasilescu, G., Arsene, M.L., Jecu, L. and Sesan, T. (2013). Optimization of Trichoderma strain cultivation for biocontrol activity. Scientific Bulletin Series F. Biotechnologies. 17: 154-159.

  10. Schuster, A. and Schmoll, M. (2010). Biology and biotechnology of Trichoderma. Applied Microbiology Biotechnology. 87: 787-799.

  11. Shaikh F.T. and Sahera, N. (2013). In vitro assessment of antagonistic activity of Trichoderma viride and Trichoderma harzianum against pathogenic fungi. Botany. 3: 179-83.

  12. Singh, A., Shahid, M., Pandey, N.K., Kumar, S., Srivastava, M. and Biswas, S. K. (2011). Influence of temperature, pH and media for growth and sporulation of Trichoderma atroviride and its shelf life study in different carrier based formulation. J. Pl. Dis. Sci. 6: 32-34.

  13. Yadav, L.S. (2012). Antagonistic activity of Trichoderma sp. and evaluation of various agro-wastes for mass production. Indian J. Pl. Sc. 1:109-112.

     
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