Antifungal activity of Acacia tortilis subsp. raddiana tar on Fusarium oxysporum f.sp. albedinis, the cause of Bayoud disease of the date palm in Southwest Algeria

DOI: 10.18805/IJARe.A-417    | Article Id: A-417 | Page : 713-717
Citation :- Antifungal activity of Acacia tortilis subsp. raddiana tar on Fusarium oxysporum f.sp. albedinis, the cause of Bayoud disease of the date palm in Southwest Algeria.Indian Journal Of Agricultural Research.2019.(53):713-717
A. Mezouari, A. Makhloufi, K. Bendjima, L. Benlarbi, A. Boulanouar, K. Makhloufi and M.D. Jesùs Gonzalez makhloufi_75@yahoo.fr
Address : Department of Biology, Faculty of Natural sciences and life, University Tahri Mohamed, Bechar, Algeria.
Submitted Date : 25-01-2019
Accepted Date : 29-06-2019

Abstract

Bayoud caused by Fusarium oxysporum f.sp. albedinis (Foa), is the most destructive disease of the date palm (Phoenix dactylifera L.) in Morocco and Algeria and there is no effective control strategy. We found that although Foa isolates vary morphologically, Foa strains can be identified by species-specific primers. PCR analysis revealed that the strains that we isolated from infected date palm rachis were the Bayoud pathogen Foa. We used these strains to evaluate the antifungal activity of tar extracted from Acacia tortilis subsp. raddiana. The A. raddiana tar had a density of 1.15, a refraction index of 1.3850, a pH of 5.2 and a dried matter ratio of 48.75%. The A. raddiana tar effectively inhibited the growth of Foa in vitro with a minimum inhibitory concentration of 3 µg/ml.

Keywords

Antifungal Bayoud diseas Date palm

References

  1. AFNOR NFT 04-408. (1974). Determination of pH. Association Française de Normalisation AFNOR Paris
  2. AFNOR NFT 60-204. (1985). Determination of Acid Value and Acidity (Titration Methods). French. Association for Standardization AFNOR, Paris.
  3. AFNOR NFT 60-212. (1968). Determination of Refractive index. Association Française de Normalisation AFNOR, Paris.
  4. AFNOR NFT 60-214. (1969). Determination of density at 20°C. Association Française de Normalisation AFNOR, Paris.
  5. Assigbeste, K. (1989). Etude de la variabilité spontanée chez le Fusarium oxysporum f.sp vasinfectum (ATK) SN et H. : agent de la Fusariose du Cotonnier. D.E.A. de phytopathologie. Université PARIS XI, Université PARIS VI, I.N.A.P.G Montpellier. 
  6. Benabbes, R., Lahmass, I., Souna, F., Chafi, A., El Youbi, E., Saalaoui, A., Hakkou, M.and Bouakka. (2015). Confrontation of antagonistic fungi selected from the soil of the Figuig oasis to date palm Fusarium f.sp. albedinis. Moroccan J. Biol., 12: 1-8.
  7. Benlarbi, L., Makhloufi, A., Tarfaya, B. and Belahcene, M. (2014). Biological activities of Olea europea sylvestris Tar, growing wild in South west of Algeria. Int. J. Cur. Microbiol. Appl. Sci., 3(8): 771-777.
  8. Bhandari, D.K., Nath, G., Ray, A.B. and Tewari, P.V. (2000). Antimicrobial activity of crude extracts from Berberis Asiatica stem bark. Pharm. Biol., 38(4): 254-257.
  9. Bhutani, N., Maheshwari, R. and Suneja, P. (2018). Isolation and characterization of plant growth promoting endophytic bacteria isolated from Vigna radiata. Indian J. Agric. Res., 52(6): 596-603.
  10. Dihazi, A., Jaiti, F., Taktak, W. and kilani-Feki, O. (2012). Use of two bacteria for biological control of bayoud disease caused by Fusarium oxysporum in date palm (Phoenix dactylifera L) seedlings. Plant Physiol. Biochem., 55: 7-15.
  11. Dihazi, A., Jaiti, F, Zouine J., El Hassni, M. and El Hadrami, I. (2003). Effect of salicylic acid on phenolic compounds related to date palm resistance to Fusarium oxysporum f. sp. albedinis. Phytopatholo. Mediterr., 42: 9-16.
  12. Edel, V., Steinberg, C., Gautheron, N. and Alabouvette, C. (2000). Ribosomal DNA-Targeted Oligonucleotide Probe And PCR Assay Specific For Fusarium Oxysporum. Mycol. Res., 104(5): 518-526.
  13. EL Hassani, M., EL Hadrami, A., Daayf, F., Ait Barka, E. and EL Hadrami, I. (2004). Chitosan, antifungal product against Fusarium oxysporum f. sp. albedinis and elicitor of defence reactions in date palm roots. Phytopathol. Mediterr., 43: 195-204.
  14. Elliott, M.L., Des Jardin, E.A., O’Donnell, K., Geiser, D.M., Harrison, N.A. and Broschat, T.K. (2010). Fusarium oxysporum f. sp. palmarum, a novel forma specialis causing a lethal disease of Syagrus romanzoffiana and Washingtonia robusta in Florida. Plant Dis., 94: 31-38.
  15. Fernandez, D., Quinten, M., Tantaoui, A., Geiger, J.P., Daboussi M. J. and Langin, T. (1998). Fot1 insertions In the Fusarium oxysporum f.sp. albedinis genome provide diagnostic PCR targets for detection of the date palm pathogen. Appl. Environ. Microbiol., 64: 633-636.
  16. Fitrianingsih, A., Martanto, E.A. and Abbas B. (2019). The effectiveness of fungi Gliocladium fimbriatum and Trichoderma viride to control fusarium wilt disease of tomatoes (Lycopersicum esculentum). Indian J. Agric. Res., 53 (1): 57-61.
  17. Freeman, S. and Maymon, M. (2000). Reliable detection of the fungal pathogen Fusarium oxysporum f.sp. albedinis, causal agent of Bayoud disease of date palm, using molecular techniques. Phytoparasitica, 28(4): 341-348.
  18. Grouzis, M. (2003). Le Floc’H E. Un arbre au désert: Acacia raddiana. IRD Editions, 313:42
  19. Imazaki, I. and Kadota, I. (2015). Molecular phylogeny and diversity of Fusarium endophytes isolated from tomato stems. FEMS Microbiol. Ecol., 91(9): fiv098.
  20. Kaboré, B.K., Couture, L., Dostaler, D and Bernier, L. (2001). Variabilité phénétique du Colletotrichum graminicola du sorgho. Can. J. Plant Pathol., 23:138-145
  21. Karkachi, N., Gharbi, S., Kihal, M. and Henni, J. (2014). Study of pectinolytic activity of Fusarium oxysporum f.sp albedinis agent responsible for bayoud in Algeria. Int. J. Agron. Agri. Res., 5(2): 40-45.
  22. Kettout, T.A and Rahmania, F. (2010). Identification of phenylacetic acid produced by Fusarium oxysporum f. sp. albedinis, the causal agent of bayoud, using GC-MS. C. R. Biologies., 333: 808-813.
  23. Kistler, H. C. (1997). Genetic diversity in the plant-pathogenic fungus Fusarium oxysporum. J. Phytopathol., 87(4): 474-479.
  24. Mahadik, V.B. and Mali, N.S. (2018). Antioxidant activity in safflower (Carthamus tinctorius L.) cultivars under the pathogenesis of foliar fungal disease complex. Indian J. Agric. Res., 52(1): 76-80.
  25. Patel, J.J. and Brown, M.E. (1969). Interactions of Azotobacter with rhizosphere and root surface microflora. Plant Soil., 31: 273-281.
  26. Rageot, M. (2018). Les substances naturelles en méditerranée Nord-occidentale (VIe- Ier Millénairebce) : chimie et archéologie des matériaux exploités pour leurs propriétés adhésives et hydrophobes. Université Nice Sophia – Antipolis-CEPAM, UMR 7264, CNRS.
  27. Rosado, Á.C., Molinero, R. L, Rodríguez, A. R. and Basallote, U. M.J. (2014). Antifungal activity of asparagus extracts against phytopathogenic Fusarium oxysporum. Sci. Hortic., 171: 51-57.
  28. Rosca, C. O., Parvu, M., Vlase, L. and Tamas, M. (2007). Antifungal activity of Aloe vera leaves. Fitoterapia, 78: 219-222.
  29. Rusli, M.H. (2012). Detection, control and resistance expression in oil palm (elaeis guineensis) caused by F.oxysporum f.sp. elaeidis, Ph.D., University of Bath.
  30. Sghir, F., Touati, J., Chliyeh, M. and Ouazzani, T. A. (2014). Diversity of arbuscular mycorrhizal fungi in the rhizosphere of date palm tree (Phoenix dactylifera) in Tafilalt and Zagora regions (Morocco). Inter. J. Pure. App. Biosci., 2 (6): 1-11.
  31. Shabani, F., Kumar, L. and Esmaeili, A. (2014). Future distributions of Fusarium oxysporum f. spp. In European, Middle Eastern and North African agricultural regions under climate change. Agriculture, Ecosystems and Environment, 197: 96-105
  32. Subba, A.R. and Rai, S.K. (2018). Phytochemical screening, Physico-chemical analysis and antioxidant activity of some ethnomedicinal plants from Sikkim Himalaya. Indian J Nat Prod Resour., 9(3): 235-243.
  33. Tantaoui, A., Ouinten, M., Geiger, J.P. and Fernandez, D. (1996). Characterization of a single clonal lineage of FOA causing Bayoud disease of date palm in Morocco. Phytopathology, 86: 787-792.
  34. Terfaya, B., Makhloufi, A., Mekboul, A., Benlarbi, L. and Abdelouahed, D. (2017). Antifungal activity of Juniperus oxycedrus Tar; growing wild in north-west of Algeria. Applied Biology in Saharan Areas, 1: 33-36.
  35. Tivoli, B. (1988). Guide to identify the different species or varieties of Fusarium encountered in France on the potato and in its environment. Agronomy, EDP Sciences, 8 (3): 211-222.
  36. Yezli, W., Zebboudj, Y. N., Hamini, K. N, Kihal, M. and Henni, J. (2015). In vitro antagonistic activity evaluation of rhizobacteria against Fusarium oxysporum f. sp. radicis-lycopersici (Forl) isolated from the Algerian west. Int. J. Biosci., 5: 115-125.
  37. Zebboudj, N., Yezli, W., Hamini, K. N., Kihal, M. and Henni, J. (2014). Antifungal activity of lactic acid bacteria against Fusarium oxysporum f. sp. albedinis isolated from diseased date palm in South Algeria. Int. J. Biosci., 5(9): 99-106 

Global Footprints