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Current IssueEditorial BoardOnline First Articles
Indian Journal of
Agricultural Research
Print ISSN: 0367-8245
Online ISSN: 0976-058X
NASS Rating
5.60
SJR
0.217
CiteScore
0.595

Chief Editor:
V. Geethalakshmi
Tamil Nadu Agricultural University Coimbatore, INDIA
Frequency:Monthly
Indexing:
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Go...

Research Article

Indian Journal of Agricultural Research, volume 53 issue 4 (august 2019) : 478-482

Changes in growth, biochemical components and antioxidant genes expression in rice seedling (Oryza sativa L.) cultivar ‘IR64’ under salt stress

M. Pharmawati, I.M.A.S. Wijaya
1Biology Department, Faculty of Mathematics and Natural Sciences, Udayana University, Kampus Bukit Jimbaran, Bali, 80361, Indonesia.
Cite article:- Pharmawati M., Wijaya I.M.A.S. (2019). Changes in growth, biochemical components and antioxidant genes expression in rice seedling (Oryza sativa L.) cultivar ‘IR64’ under salt stress. Indian Journal of Agricultural Research. 53(4): 478-482. doi: 10.18805/IJARe.A-399.

ABSTRACT

One of abiotic stresses that affects rice growth is salinity.  Plant must develop adaptation process which includes morphological, biochemical and molecular changes.  This research aimed to evaluate morpho-biochemical and molecular responses of rice ‘IR64’ to several levels of salinity stress at seedling stage.  Seedlings of ‘IR64’ were grown in a hydroponic system and treated with different levels of salinity stress (4dSm-1, 6dSm-1, and 12dSm-1) for seven days.  Responses were recorded on the final day of salt treatments. Gene expression analyses were done by semi-quantitative RT-PCR. RNA was extracted using RNase plant mini kit (Qiagen) and cDNA was synthesized using GoSript™ Reverse Transcription System (Promega). Results showed that shoot height and fresh weight decreased under salt stress.  At plants treated with salt, the chlorophyll contents were lower than that of control plants, while MDA levels were higher in salt treated plants. Semi-quantitative PCR for MnSOD1and cCu/ZnSOD1 revealed that MnSOD1 and cCu/ZnSOD1expressions increased under salt stress which indicated oxidative stress defence, with the highest expression at 4dSm-1and 6dSm-1 treatment, respectively.

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