Indian Journal of Agricultural Research

  • Chief EditorV. Geethalakshmi

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

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Indian Journal of Agricultural Research, volume 49 issue 4 (august 2015) : 373-376

Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers

Manoj Kumar Sao, S.K. Nair, S.B. Verulkar, R.R. Saxena, H.C. Nanda
1Department of Genetics and Plant Breeding, IGKV, Raipur-492 012, India.
Cite article:- Sao Kumar Manoj, Nair S.K., Verulkar S.B., Saxena R.R., Nanda H.C. (2024). Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers. Indian Journal of Agricultural Research. 49(4): 373-376. doi: 10.5958/0976-058X.2015.00068.2.

ABSTRACT

Five lines of four mungbean genotypes were analyzed for polymorphism using a total of five Inter Simple Sequence Repeat (ISSR) and two Simple Sequence Repeat (SSR) markers. Unique alleles differentiating the genotypes could be identified. Amplification of genomic DNA of most popular four Indian mungbean genotypes with these ISSR and SSR primers yielded 162 fragments that could be scored, of which 56 were polymorphic, with an average of 8.0 polymorphic fragments per primer. Number of amplified bands with ISSR and SSR primers ranged from 7 to 37. Percentage polymorphism ranged from 28.56 (UBC 836) to 57.14 (SSR 2). Polymorphic Information Content ranged from 0.584 (SSR 2) to 0.907 (UBC 809). The results indicated that both of the markers ISSR and SSR individually can be effectively used in determination of genetic relationship among Vigna species. It could be concluded that, the information of genetic similarities and diversity among Vigna genotypes can easily be identified using molecular techniques.

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