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volume 49 issue 4 (august 2015) : 373-376,   Doi: 10.5958/0976-058X.2015.00068.2

Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers

M
Manoj Kumar Sao
S
S.K. Nair
S
S.B. Verulkar
R
R.R. Saxena
H
H.C. Nanda
1Department of Genetics and Plant Breeding, IGKV, Raipur-492 012, India.
Cite article:- Sao Kumar Manoj, Nair S.K., Verulkar S.B., Saxena R.R., Nanda H.C. (2025). Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers. Indian Journal of Agricultural Research. 49(4): 373-376. doi: 10.5958/0976-058X.2015.00068.2.

ABSTRACT

Five lines of four mungbean genotypes were analyzed for polymorphism using a total of five Inter Simple Sequence Repeat (ISSR) and two Simple Sequence Repeat (SSR) markers. Unique alleles differentiating the genotypes could be identified. Amplification of genomic DNA of most popular four Indian mungbean genotypes with these ISSR and SSR primers yielded 162 fragments that could be scored, of which 56 were polymorphic, with an average of 8.0 polymorphic fragments per primer. Number of amplified bands with ISSR and SSR primers ranged from 7 to 37. Percentage polymorphism ranged from 28.56 (UBC 836) to 57.14 (SSR 2). Polymorphic Information Content ranged from 0.584 (SSR 2) to 0.907 (UBC 809). The results indicated that both of the markers ISSR and SSR individually can be effectively used in determination of genetic relationship among Vigna species. It could be concluded that, the information of genetic similarities and diversity among Vigna genotypes can easily be identified using molecular techniques.

REFERENCES

  1. Ajibade, S. R., Weeden, N. F. and Chite, S.M., (2000). Inter simple sequence repeat analysis of genetic relationships in the genus Vigna. Euphytica 111: 47-55.
  2. Autrique, E., Nachit, M. M., Monneveux, P., Tanksley, S. D. and Sorrells, M. E. (1996). Genetic diversity in durum wheat based on RFLPs, morphological traits, and coefficient of parentage. Crop Sci. 36: 735-742.
  3. Jonathan, F. W. and Wendel, N.F. (1990). Visualization and interpretation of plant isozyme In: Isozyme in Plant Biology. [D.E. Sdtis and P.S. Sottis (eds.)]. Champan and Hall. London pp. 5-45.
  4. Litt, M. and Luty, J. A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Human Genet. 44: 397.
  5. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G. and Erlich, H. (1986). Specific enzymatic amplification of DNA in vitro the polymerase chain reaction. Cold Spring Harb. Symp. Quant. Biol. 51: 263-273.
  6. Rohlf, F. J. (2000). NYSYS numerical taxonomy and multivariate analysis systemversion 2.2 user guide, Applied Biostatistics, Inc., New York.
  7. Souframanien, J. and Gopalakrishna, T. (2004). A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor. Appl. Genet. 109: 1687-1693.
  8. Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl.eic Acids Res. 17: 6463-6471.
  9. Weber, J. L. and May, P. E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Human Genet. 44: 388.
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Indian Journal of Agricultural Research
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    volume 49 issue 4 (august 2015) : 373-376,   Doi: 10.5958/0976-058X.2015.00068.2

    Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers

    M
    Manoj Kumar Sao
    S
    S.K. Nair
    S
    S.B. Verulkar
    R
    R.R. Saxena
    H
    H.C. Nanda
    1Department of Genetics and Plant Breeding, IGKV, Raipur-492 012, India.
    Cite article:- Sao Kumar Manoj, Nair S.K., Verulkar S.B., Saxena R.R., Nanda H.C. (2025). Molecular profiling and genetic diversity of mungbean (Vigna radiata L.) genotypes using ISSR and SSR markers. Indian Journal of Agricultural Research. 49(4): 373-376. doi: 10.5958/0976-058X.2015.00068.2.

    ABSTRACT

    Five lines of four mungbean genotypes were analyzed for polymorphism using a total of five Inter Simple Sequence Repeat (ISSR) and two Simple Sequence Repeat (SSR) markers. Unique alleles differentiating the genotypes could be identified. Amplification of genomic DNA of most popular four Indian mungbean genotypes with these ISSR and SSR primers yielded 162 fragments that could be scored, of which 56 were polymorphic, with an average of 8.0 polymorphic fragments per primer. Number of amplified bands with ISSR and SSR primers ranged from 7 to 37. Percentage polymorphism ranged from 28.56 (UBC 836) to 57.14 (SSR 2). Polymorphic Information Content ranged from 0.584 (SSR 2) to 0.907 (UBC 809). The results indicated that both of the markers ISSR and SSR individually can be effectively used in determination of genetic relationship among Vigna species. It could be concluded that, the information of genetic similarities and diversity among Vigna genotypes can easily be identified using molecular techniques.

    REFERENCES

    1. Ajibade, S. R., Weeden, N. F. and Chite, S.M., (2000). Inter simple sequence repeat analysis of genetic relationships in the genus Vigna. Euphytica 111: 47-55.
    2. Autrique, E., Nachit, M. M., Monneveux, P., Tanksley, S. D. and Sorrells, M. E. (1996). Genetic diversity in durum wheat based on RFLPs, morphological traits, and coefficient of parentage. Crop Sci. 36: 735-742.
    3. Jonathan, F. W. and Wendel, N.F. (1990). Visualization and interpretation of plant isozyme In: Isozyme in Plant Biology. [D.E. Sdtis and P.S. Sottis (eds.)]. Champan and Hall. London pp. 5-45.
    4. Litt, M. and Luty, J. A. (1989). A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene. Am. J. Human Genet. 44: 397.
    5. Mullis, K., Faloona, F., Scharf, S., Saiki, R., Horn, G. and Erlich, H. (1986). Specific enzymatic amplification of DNA in vitro the polymerase chain reaction. Cold Spring Harb. Symp. Quant. Biol. 51: 263-273.
    6. Rohlf, F. J. (2000). NYSYS numerical taxonomy and multivariate analysis systemversion 2.2 user guide, Applied Biostatistics, Inc., New York.
    7. Souframanien, J. and Gopalakrishna, T. (2004). A comparative analysis of genetic diversity in blackgram genotypes using RAPD and ISSR markers. Theor. Appl. Genet. 109: 1687-1693.
    8. Tautz, D. (1989). Hypervariability of simple sequences as a general source for polymorphic DNA markers. Nucl.eic Acids Res. 17: 6463-6471.
    9. Weber, J. L. and May, P. E. (1989). Abundant class of human DNA polymorphisms which can be typed using the polymerase chain reaction. Am. J. Human Genet. 44: 388.
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