To study the influence of plant growth regulators, micronutrients and stage of harvesting on seed maturity and quality in African marigold cv. Pusa Narangi Gainda a field experiment was conducted at Floriculture Research Station, Rajendranagar, Hyderabad during the rabi season of 2011-12. The experiment was laid out in randomized block design with twelve treatments and replicated thrice. The treatments consisted of GA3 at 200 ppm, NAA at 100 ppm, MH at 1000 ppm, ZnSO4 at 0.5%, Boron at 0.2%, GA3 at 200 ppm + ZnSO4 at 0.5%, GA3 at 200 ppm + boron at 0.2%, NAA at 100 ppm + ZnSO4 at 0.5%, NAA at 100 ppm + boron at 0.2%, MH at 1000 ppm + ZnSO4 at 0.5%, MH at 1000 ppm + boron at 0.2% and control. Treatments were imposed as foliar sprays at 15, 30 and 45 days after transplanting. Data on various seed maturity and quality parameters was recorded at 14, 21, 28, 35 and 42 days after anthesis (DAA). Maximum flower weight (4.42 g), seed yield per flower (0.99 g), number of filled seeds (200.13), thousand seed weight (3.14 g), germination percentage (77.18%), seedling length (8.75 cm) and seedling vigour index (695) were recorded with the treatment MH at 1000 ppm + boron at 0.2% and was found to be on par with the treatment GA3 at 200 ppm. The treatment NAA at 100 ppm + boron at 0.2% was also found on par with MH at 1000 ppm + boron at 0.2% in respect of flower weight, thousand seed weight, seedling length and seedling vigour index. Lowest number of unfilled seeds (61.67) was recorded with the treatment MH at 1000 ppm + ZnSO4 at 0.5% to which treatments ZnSO4 at 0.5%, Boron at 0.2%, GA3 at 200 ppm + boron at 0.2% and NAA at 100 ppm + boron at 0.2% were found on par. Among various DAA, highest flower weight (4.40 g), seed yield per flower (0.69 g), number of filled seeds (173.56), thousand seed weight (2.93 g), germination percentage (83.25), seedling length (9.59 cm) and seedling vigour index (799) were recorded at 28 DAA. Minimum number of unfilled seeds (48.19) was registered at 42 DAA.