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volume 48 issue 6 (december 2014) : 459-464,   Doi: 10.5958/0976-058X.2014.01330.4

SCREENING AND IDENTIFICATION OF POTENTIAL TRICHODERMA SP. AGAINST SOIL BORNE PATHOGENS OF VANILLA (VANILLA PLANIFOLIA)

A
Athul Sandheep R
M
M.S. Jisha*
1School of Biosciences M.G University, Kottayam-686 001, India
Cite article:- R Sandheep Athul, Jisha* M.S. (2025). SCREENING AND IDENTIFICATION OF POTENTIAL TRICHODERMA SP. AGAINST SOIL BORNE PATHOGENS OF VANILLA (VANILLA PLANIFOLIA) . Indian Journal of Agricultural Research. 48(6): 459-464. doi: 10.5958/0976-058X.2014.01330.4.

ABSTRACT

This study was conducted to evaluate the biocontrol potential of indigenous Trichoderma sp. against fungal pathogens of Vanilla. The pathogens causing wilt and rot diseases of vanilla Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii were isolated from naturally infected vanilla plants. Among the ten isolates, two species Trichoderma harzianum T2 and Trichoderma virens T5 which showed 80 to 90% inhibitions against the selected fungal pathogens were selected. The Trichoderma isolates were identified using  Internal transcribed spacer (ITS) region amplification and sequencing. The sequences generated in this study were deposited in the NCBI gene bank and culture collection centre and got the accession number JN 863298 for the isolate Trichoderma virens and JN 000305 for the isolate Trichoderma harzianum.The compatibility of the bioagents with selected fungicides were also investigated under in vitro conditions.Bordeaux mixture was found to be inhibitory to Trichoderma at all given concentrations, whereas the bioagents found to be compatible with potassium phosphonate. Trichoderma harzianum inoculation gave 90-95 % disease suppression under green house experiment  and was superior than Trichoderma virens and the standard  (MTCC 801). Application of biocontrol agents for crop protection is very significant as it has several advantages such as possibility of multiple pathogen suppression, low cost and promotion of soil fertility

REFERENCES

  1. Ainhoa Martínez-Medina, Antonio Roldán and Jose A. Pascual. (2011). Interaction between arbuscular mycorrhizal fungi and Trichoderma harzianum under conventional and low input fertilization field condition in melon crops: Growth response and Fusarium wilt biocontrol. Applied Soil Ecology 4:7 98–105.
  2. Divakaran, M., Pillai, G.S., Babu, K.N., and Peter, K.V. (2008). Isolation and fusion of protoplasts in Vanilla species. Current Science, 94(1): 115-120.
  3. Deb, P.R. and Dutta B. K. (1991).Studies on biological control of foot rot disease of soyabean caused by Sclerotium rolfsi i Sacc. Zeitschri f fuer Pflanzenkrankheiten and Pflanzenschutz.,98:733-745
  4. Dubey, S.C. and Patel, B. (2001). Determination of tolerance in Thanatephorus cucumeris, Trichoderma viride, Gliocladium virens and Rhizobium sp. to fungicides. Indian Phytopathol., 54:98 – 101.
  5. Fakhrunnisa, Hashmi. M. H. and Ghaffar, A. (2006), In Vitro interaction of Fusarium spp., with their fungi. Pak.J. Bot., 38(4):1317-1322.
  6. Ganesan, P. and Gnanamanickam, S. S. (1987), Biological control of Sclerotium rolfsii Sacc. In peanut by inoculation with Pseudomonas fluorescens. Soil Bio. Biochem., 19(1) : 35-38.
  7. Grinyer, J., McKay ,M., Nevalainen H. and Herbert B. R. (2004). Fungal proteomics: initial mapping of biological control strain Trichoderma harzianum. Curr Genet. Mar.,45(3):163-9.
  8. Harman, G. E., Howell,C.R., Viterbo,A., Chet,I. and Lorito,I.M. (2004), Trichoderma species-Opportunistic, avirulent plant symbionts. Nature Rev., 2:43-56.
  9. Jadhav, D., Rekha, B. N., Gogate, P. R., and Rathod, V. K. (2009). Extraction of vanillin from vanilla pods: A comparison study of conventional soxhlet and ultrasound assisted extraction. J Food Eng., 93: 421-426.
  10. Johnson, L.F. and Curl, E.A. (1972). Methods for Research on Ecology of Soil-borne Plant Pathogens. Burgers Publishing Co., Minneapolis, 247p.
  11. Kuruvilla, R., Zweifel, L. S., Glebova, N. O., Lonze, B. E., Valdez, G., Ye, H. and Ginty, D. D. (2004). A neurotrophin signaling cascade coordinates sympathetic neuron development through differential control of TrkA trafficking and retrograde signaling. Cell ,118:243-255
  12. Manjula ,K., Krishna Kishore, G., Girish A. G. and Singh, S. D. (2004).Combined Application of Pseudomonas fluorescens and Trichoderma viride has an Improved Biocontrol Activity Against Stem Rot in Groundnut.Plant Pathol J.,20(1): 75-80
  13. Naik, G. B., Nagaraja R., Basavaraja M.K. and Krishna Naik,K. R. (2010), Variability studies of Fusarium oxysporum f. Sp. Vanillae isolates. Int.J.Sci.nature., 1(1): 12-16.
  14. Niknejad, M., Sharfi-Tehani, A. and Okhovat, M. (2000). Effect of antagonistic fungi Trichoderma spp. on the control of Fusarium wilt of tomato caused Fusarium oxysporum f. sp. lycopersici under greenhouse conditions. Iranian Agric. Sci., 1: 31 - 37.
  15. Radjacommare, R., Venkatesan,S. and Samiyappan,R. (2010).Biological control of phytopathogenic fungi of vanilla through lytic action of Trichoderma species and Pseudomonas fluorescens .Phytopathology and Plant Protection, 43(1): 1–17
  16. Rajeswari P. and Kannabiran B. (2011), In vitro effects of antagonistic microorganisms on Fusarium oxysporum (Schlecht. Emend. Synd & Hans) infecting Arachis hypogaea L. J. Phytol., 3 (3):83-85.
  17. Riad S. R.,El-Mohamedy, E. H.,Abd El-Samad., Hoda A., Habib,M and Fath El-Bab ,T. Sh. (2011). Effect of using bio-control agents on growth, yield, head quality and root rot control in broccoli plants.,Int.J.Academic Res., 3(2):71-80  
  18. Rifai, M. A. (1969). A revision of genus Trichoderma. Mycological papers No. 116. Java. Indoneshia. 65p
  19. Shahida, K., surendra Gopal,K. and Sally .K.Mathew (2010).Phytophthora meedii causing Phytophthora rot in Vanilla and its compatibility with fungicides. SAARC J Agri., 8(3): 103-111
  20. Skidmore, A. M. and Dickson, C. M. (1976), Colony interactions and hyphae interferences between Septoria nodorum and phylloplane fungi. Trans.Br.Mycol.Soc., 66:57-64.
  21. Srivastava, R., Khalid, A., Singh, U.S. and Sharma, A.K. (2010), Evaluation of arbuscular mycorrhizal fungus, Pseudomonas fluorescens and Trichoderma harzianum formulation against Fusarium oxysporum f. sp. lycopersici for the management of tomato wilt. Bio. Control, 53: 24-31.
  22. Suseela Bhai . R. and Dhanesh, J. (2008), Occurrence of fungal diseases in vanilla (Vanilla planifolia andrews) in Kerala. Journal of Spices and Aromatic Crops , 17 (2): 140-148.
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Indian Journal of Agricultural Research
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    volume 48 issue 6 (december 2014) : 459-464,   Doi: 10.5958/0976-058X.2014.01330.4

    SCREENING AND IDENTIFICATION OF POTENTIAL TRICHODERMA SP. AGAINST SOIL BORNE PATHOGENS OF VANILLA (VANILLA PLANIFOLIA)

    A
    Athul Sandheep R
    M
    M.S. Jisha*
    1School of Biosciences M.G University, Kottayam-686 001, India
    Cite article:- R Sandheep Athul, Jisha* M.S. (2025). SCREENING AND IDENTIFICATION OF POTENTIAL TRICHODERMA SP. AGAINST SOIL BORNE PATHOGENS OF VANILLA (VANILLA PLANIFOLIA) . Indian Journal of Agricultural Research. 48(6): 459-464. doi: 10.5958/0976-058X.2014.01330.4.

    ABSTRACT

    This study was conducted to evaluate the biocontrol potential of indigenous Trichoderma sp. against fungal pathogens of Vanilla. The pathogens causing wilt and rot diseases of vanilla Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii were isolated from naturally infected vanilla plants. Among the ten isolates, two species Trichoderma harzianum T2 and Trichoderma virens T5 which showed 80 to 90% inhibitions against the selected fungal pathogens were selected. The Trichoderma isolates were identified using  Internal transcribed spacer (ITS) region amplification and sequencing. The sequences generated in this study were deposited in the NCBI gene bank and culture collection centre and got the accession number JN 863298 for the isolate Trichoderma virens and JN 000305 for the isolate Trichoderma harzianum.The compatibility of the bioagents with selected fungicides were also investigated under in vitro conditions.Bordeaux mixture was found to be inhibitory to Trichoderma at all given concentrations, whereas the bioagents found to be compatible with potassium phosphonate. Trichoderma harzianum inoculation gave 90-95 % disease suppression under green house experiment  and was superior than Trichoderma virens and the standard  (MTCC 801). Application of biocontrol agents for crop protection is very significant as it has several advantages such as possibility of multiple pathogen suppression, low cost and promotion of soil fertility

    REFERENCES

    1. Ainhoa Martínez-Medina, Antonio Roldán and Jose A. Pascual. (2011). Interaction between arbuscular mycorrhizal fungi and Trichoderma harzianum under conventional and low input fertilization field condition in melon crops: Growth response and Fusarium wilt biocontrol. Applied Soil Ecology 4:7 98–105.
    2. Divakaran, M., Pillai, G.S., Babu, K.N., and Peter, K.V. (2008). Isolation and fusion of protoplasts in Vanilla species. Current Science, 94(1): 115-120.
    3. Deb, P.R. and Dutta B. K. (1991).Studies on biological control of foot rot disease of soyabean caused by Sclerotium rolfsi i Sacc. Zeitschri f fuer Pflanzenkrankheiten and Pflanzenschutz.,98:733-745
    4. Dubey, S.C. and Patel, B. (2001). Determination of tolerance in Thanatephorus cucumeris, Trichoderma viride, Gliocladium virens and Rhizobium sp. to fungicides. Indian Phytopathol., 54:98 – 101.
    5. Fakhrunnisa, Hashmi. M. H. and Ghaffar, A. (2006), In Vitro interaction of Fusarium spp., with their fungi. Pak.J. Bot., 38(4):1317-1322.
    6. Ganesan, P. and Gnanamanickam, S. S. (1987), Biological control of Sclerotium rolfsii Sacc. In peanut by inoculation with Pseudomonas fluorescens. Soil Bio. Biochem., 19(1) : 35-38.
    7. Grinyer, J., McKay ,M., Nevalainen H. and Herbert B. R. (2004). Fungal proteomics: initial mapping of biological control strain Trichoderma harzianum. Curr Genet. Mar.,45(3):163-9.
    8. Harman, G. E., Howell,C.R., Viterbo,A., Chet,I. and Lorito,I.M. (2004), Trichoderma species-Opportunistic, avirulent plant symbionts. Nature Rev., 2:43-56.
    9. Jadhav, D., Rekha, B. N., Gogate, P. R., and Rathod, V. K. (2009). Extraction of vanillin from vanilla pods: A comparison study of conventional soxhlet and ultrasound assisted extraction. J Food Eng., 93: 421-426.
    10. Johnson, L.F. and Curl, E.A. (1972). Methods for Research on Ecology of Soil-borne Plant Pathogens. Burgers Publishing Co., Minneapolis, 247p.
    11. Kuruvilla, R., Zweifel, L. S., Glebova, N. O., Lonze, B. E., Valdez, G., Ye, H. and Ginty, D. D. (2004). A neurotrophin signaling cascade coordinates sympathetic neuron development through differential control of TrkA trafficking and retrograde signaling. Cell ,118:243-255
    12. Manjula ,K., Krishna Kishore, G., Girish A. G. and Singh, S. D. (2004).Combined Application of Pseudomonas fluorescens and Trichoderma viride has an Improved Biocontrol Activity Against Stem Rot in Groundnut.Plant Pathol J.,20(1): 75-80
    13. Naik, G. B., Nagaraja R., Basavaraja M.K. and Krishna Naik,K. R. (2010), Variability studies of Fusarium oxysporum f. Sp. Vanillae isolates. Int.J.Sci.nature., 1(1): 12-16.
    14. Niknejad, M., Sharfi-Tehani, A. and Okhovat, M. (2000). Effect of antagonistic fungi Trichoderma spp. on the control of Fusarium wilt of tomato caused Fusarium oxysporum f. sp. lycopersici under greenhouse conditions. Iranian Agric. Sci., 1: 31 - 37.
    15. Radjacommare, R., Venkatesan,S. and Samiyappan,R. (2010).Biological control of phytopathogenic fungi of vanilla through lytic action of Trichoderma species and Pseudomonas fluorescens .Phytopathology and Plant Protection, 43(1): 1–17
    16. Rajeswari P. and Kannabiran B. (2011), In vitro effects of antagonistic microorganisms on Fusarium oxysporum (Schlecht. Emend. Synd & Hans) infecting Arachis hypogaea L. J. Phytol., 3 (3):83-85.
    17. Riad S. R.,El-Mohamedy, E. H.,Abd El-Samad., Hoda A., Habib,M and Fath El-Bab ,T. Sh. (2011). Effect of using bio-control agents on growth, yield, head quality and root rot control in broccoli plants.,Int.J.Academic Res., 3(2):71-80  
    18. Rifai, M. A. (1969). A revision of genus Trichoderma. Mycological papers No. 116. Java. Indoneshia. 65p
    19. Shahida, K., surendra Gopal,K. and Sally .K.Mathew (2010).Phytophthora meedii causing Phytophthora rot in Vanilla and its compatibility with fungicides. SAARC J Agri., 8(3): 103-111
    20. Skidmore, A. M. and Dickson, C. M. (1976), Colony interactions and hyphae interferences between Septoria nodorum and phylloplane fungi. Trans.Br.Mycol.Soc., 66:57-64.
    21. Srivastava, R., Khalid, A., Singh, U.S. and Sharma, A.K. (2010), Evaluation of arbuscular mycorrhizal fungus, Pseudomonas fluorescens and Trichoderma harzianum formulation against Fusarium oxysporum f. sp. lycopersici for the management of tomato wilt. Bio. Control, 53: 24-31.
    22. Suseela Bhai . R. and Dhanesh, J. (2008), Occurrence of fungal diseases in vanilla (Vanilla planifolia andrews) in Kerala. Journal of Spices and Aromatic Crops , 17 (2): 140-148.
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