Evaluation of water deficiency impacts on antioxidant enzymes activity and lipid peroxidation in some tomato (Solanum lycopersicum L.) Lines

DOI: 10.18805/IJARe.A-318    | Article Id: A-318 | Page : 228-235
Citation :- Evaluation of water deficiency impacts on antioxidant enzymes activity and lipid peroxidation in some tomato (Solanum lycopersicum L.) Lines.Indian Journal Of Agricultural Research.2018.(52):228-235
Maryam Noori, Alireza Motallebi Azar, Mehdi Saidi, Jaber Panahandeh and Davoud Zare Haghi hortazar@gmail.com
Address : Department of Horticultural Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
Submitted Date : 18-12-2017
Accepted Date : 29-03-2018

Abstract

This experiment was conducted to evaluate the effects of drought stress on some quality traits of tomato, seven tomato lines were assessed in an experimental field at Ilam University in 2016. The layout was split  plots according to randomized complete block design (RCBD) with 3 levels of drought stress including 100, 60% and 40% of field capacity with 3 replications. The genotypes were including King stone, Peto early, Bitstoik, LA1607, LA2656,  LA2080 and LA1579. The effect of mild and severe drought stress on the activity of antioxidant enzymes, including peroxidase, catalase and ascorbate peroxidase were investigated. The statistical analysis showed that drought stress significantly affected all assessed traits under drought condition. CAT, chlorophyll content and RWC were reduced, but, APX, POX, Proline and MAD content as well as electrolyte leakage increased and the highest content was observed under severe drought stress (40%FC). The genotypes LA1607, LA2656, LA2080 and LA1579 identified as the most tolerant and King Stone, Peto early and Bitstoik were the most susceptible genotypes. Under severe drought stress, the highest RWC in LA2080, POX in LA1579, APX in LA1607, Chlorophyll a in LA2080, Chlorophyll b in Peto early, Chlorophyll T in Bitstoik and LA2080, EL in LA2080, MDA in Bitstoik and prolin in Petoearly were observed. 

Keywords

Antioxidant Enzymes Deficit irrigation Drought stress Lipid Peroxidation.

References

  1. Abedi, T. and Pakniyat, H. (2010). Antioxidant enzyme changes in response to drought stress in ten cultivars of oil seed Rape (Brassica napus L.). Czech J. Genet. Plant Breeding. 46(1): 27–34.
  2. Aebi, H., (1984). Catalase in vitro. Methods in Enzymology. 105: 121-126. 
  3. AL Hassan, M., Martinez Fuertes, M., Ramos Sanchez F.J., Vicente O., and Boscaiu, M. (2015). Effects of salt and water stress on plant growth and on accumulation of osmolytes and antioxidant compounds in cherry tomato. Not Bot. Horti. Agrobo., 43(1):1-11. 
  4. Ankush, and Sharma, S.K. (2017). Yield, quality, nutrient and water use efficiency of tomato as affected by different fertigation rates through drip irrigation system. Indian J. Agric. Res., 51(5): 478-482.
  5. Assah, D.V.M., AkihiroUeda, L.L., Nagooka, T. and Saneoka, H. (2015). Effects of drought stress on growth, solute accumulation and memberane stability of leafy vegetable, huckleberry (Solanum scabrum Mill.). Envirom. Biol., 37:107-114. 
  6. Ashraf, U., Kanu, A.S., Mo, Z., Hussain, A., Anjum, S.A., and Khan, I. (2015). Leadoxicity in rice: effects, mechanism and mitigation strategies-a mini review. Environ. Sci. Pollut. Res., 22:18318-18332.
  7. Bates, L.S., Walderren R.P. and Teare, I. D. (1973). Rapid determination of free proline for water- studies. Plant. Soil., 39:205-207. 
  8. Chance, B. and Maehly, A.C. (1995). Assay of catalases and peroxidases. Methods Enzymol., 2:764-817. 
  9. Cunhua, S., Wei, D., Xiangling, C., Xinna, X., Yahong, Z., Dong, S., and Jianjie, S. (2010). The effects of drought stress on the activity of acid Phosphatase and its Protective Enzymes in Pig weed Leaves. Afr. J. Biotechnol., 9: 825-833. 
  10. Demidchik, V., Straltsova, D., Medvedev, S.S., Pozhvanov, G. A., Sokolik, A. and Yurin, V. (2014). Stress-induced electrolyte leakage: the role of K+-permeable channels and involvement in programmed cell death and metabolic adjustment. J. Exp. Bot. Vol., 65(4). 1259-1270.
  11. Fahimirad, M., Karimzadeh, G. and Ghanati, F. (2013). Cold-induced changes of antioxidant enzymes activity and lipid peroxidation in two canola (Brassica napus L.) cultivars. J. Plant Physiol. Breed., 3(1):1-11. 
  12. Ghorbanli, M., Gafarabad, M., Amirkian, T. and Allahverdi, Mamaghani, B., (2013). Investigation of proline, total protein, chlorophyll, ascorbate and dehydroascorbate changes under drought stress in Akria and Mobil tomato cultivars. Iran. J. Plant Physiol., 3(2):651-658. 
  13. Hassan Khan, S., Khan, A., Litaf, U., Sattar Shah, A., Ali Khan, M., Bilal, M. and Usman, Ali M. (2015). Effect of drought stress on tomato cv. Bombino. J. Food Process Technol., 6:465.
  14. Huang, Y., Zhixiong, L., Zhilong, B., Ai, Z. and Wang, W. (2009). Protective role of proline against salt stress is partially related to the improvement of water status and peroxidase enzyme activity in cucumber. Soil Sci. Plant Nutr., 55:698–708.
  15. Khan, S.U., Gurmani, A.R.J.D., Qayyum, A., Abbasi, K.S., Liaquat, M. and Ahmad, Z., (2016). Exogenously applied gibberelic acid, indolic acetic acid and kinetin as potential regulators of source-sink relationship, physiological and yield attributes in rice (Oryza sativa) genotypes under water deficit conditions. J. Agric. Biol., 18(1):135-145.
  16. Korkmaz, A., Korkmaz, Y., and Demirkiran, A.R., (2010). Enhancing chilling stress tolerance of pepper seedling by exogenous application of 5-aminolevolinic acid. Environ. Exper. Bot., 67:495-501. 
  17. Lum, M.S., Hanafi, M.M., Rafii, Y. M. and Akma,r A.S.N. (2014). Effect of drought stress on Growth, proline and antioxidant enzyme activities of upland rice. J. Animal and Plant Sciences., 24(5):1487-1493.
  18. Mafakheri, A., Siosemardeh, A., Bahramnejad, B., Struik, P.C. and Sohrabi, Y. (2010). Effect of drought stress on yield, proline and chlorophyll contents in three chickpea cultivars. Aust. J. Crop Sci., 4:580-585. 
  19. Masoumi, A., Kafi, M., Khazaei, H. and Davari, K., (2010). Effect of drought stress on water status, electrolyte leakage and enzymatic antioxidants of kochia (Kochia Scoparia) under saline condition. Pak. J. Bot., 42(5):3517-3524. 
  20. Massacci, A., Nabiev, S.M., Pietrosanti, N., Nematov, S.K., Chernikova, T.N., Thor, K. and Leipner, J. (2008). Response of the photosynthetic capparatus of cotton (Gossypium hirsutum) to the onset of drought stress under field conditions studied by gas-exchange analysis and chlorophyll fluorescence imaging. Plant Physiol. Biochem., 46:189-195. 
  21. Meher, P., Shivakrishna, M.P., Reddy, K.A. and Rao, D.M. (2017). Effect of PEG-6000 imposed drought stress on RNA content, relative water content (RWC), and chlorophyll content in pea nut leaves and roots. Saudi Journal of Biological Sciences. https://doi.org/10.1016/j.sjbs.2017.04.008
  22. Mohaewsh, O. (2016). Utilizing deficit irrigation to enhance growth performance and water- use efficiency of eggplant in arid environments. J. Agr. Sci. Tech., 18:265-276.
  23. Nxele, X., Klein, A. and Ndimba, B.K. (2017). Drought and salinity stress alters ROS accumulation, water retention, and osmolyte content in sorghum plants. S. Afr J. Botan., 108:261-266.
  24. Omidi, H., (2010). Changes of prolin content and activity of antioxidative enzymes in two canola genotype under drought stress. Amer. J. Plant Physiol., 5:338-349.
  25. Saneoka, H., Moghaieb, R.E.A., Premachandra, G.S. and Fujita, K. (2004). Nitrogen nutrition and water stress effects on cell membrane stability and leaf water relations in Agrostis palustris Huds. Environmental and Experimental Botany, 52:131-138. 
  26. Sathya, S., Mani, S., Mahendran, P.P. and Arulmozhiselvan, K., (2010). Effect of application of boron on growth, quality and fruit yield of PKM tomato. Indian J. Agric. Res., 44 (4):274-280.
  27. Slamet, W., Purbajanti, E. D., Darmawati, A. and Fuskhah, E., (2017). Leaf area index, chlorophyll, photosynthesis rate of lettuce (Lactuca sativa L.) under N-organic fertilizer. Indian J. Agric. Res., 51(4):365-369.
  28. Soltys-Kalina, D., Plich J., Strzelczyk-Zyta, D., Sliwka J. and Marczewski, W. (2016). The effect of drought stress on the leaf relative water content and tuber yield of a half-sib family of Katahdin-derived potato cultivars. Breed Sci., 66(2):328–331. 

Global Footprints