Indian Journal of Agricultural Research

  • Chief EditorV. Geethalakshmi

  • Print ISSN 0367-8245

  • Online ISSN 0976-058X

  • NAAS Rating 5.60

  • SJR 0.293

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Agricultural Research, volume 50 issue 3 (june 2016) : 214-219

Genetic characterization of Spanish lentil landraces (Lens culinaris Medik.) by biochemical markers

M.D. Cristóbal, B. Herrero*
1<p>University of Valladolid, Escuela de Ingenier&iacute;as Agrarias,&nbsp;Avenida de Madrid 57, 34004 Palencia, Spain.</p>
Cite article:- Crist&oacute;bal M.D., Herrero* B. (2016). Genetic characterization of Spanish lentil landraces (Lens culinaris Medik.) by biochemical markers . Indian Journal of Agricultural Research. 50(3): 214-219. doi: 10.18805/ijare.v50i3.10743.

ABSTRACT

Diversity analysis and a genetic structure study of 25 lentil landraces (Lens culinaris Medik.) from the northern east part of the Iberian Peninsula were carried out. 8 isozymes: PGI, PRX, LAP, 6PGD, GOT, SKDH, PGM and ME, have been used for this purpose having found 17 loci for them, with 52.94% polymorphic loci and a total amount of 21 alleles. All populations show between 18 and 26 alleles. Genetic distance (D) was 0.123. Diversity parameters show similar or higher levels compared to other autogamous plant populations. They show an heterozygote defect. Coefficient of homozygotes among populations (GST) value 0.414, was similar to Inbreeding coefficient among populations (FST) that was 0.454. Main component analysis showed that the most important isozymes in the characterization process were GOT-3 and PGM-2. Four groups of populations were obtained by cluster analysis. This information reveals new data for improving their use in the germplasm banks for crop programs.

REFERENCES


  1. microsatellite markers for lentil (Lens culinaris Medik.). Plant Breed. 134: 589-598

  2. Bacchi, M., Leone, M., Mercati, F., Preiti, G., Sunseri, F. and Monti, M. (2010). Agronomic evaluation and genetic characterization of different accessions in lentil (Lens culinaris Medik.). Ital. J. Agron. 4: 303-314.

  3. Brown, A.H.D. (1989). Core collection: a practical approach to genetic resources management. Genome 31: 818-824.

  4. Cardy, B.J., Stuber, C.W. and Goodman, M.M. (1980). Techniques for starch gel electrophoresis of enzymes from maize (Zea mays L.). North Carolina State University, Raleigh.

  5. Erdogan, C. 2015. Genetic characterization and cotyledon color in lentil. Chil. J. Agr. Res. 75: 383-389.

  6. Erskine, W. and Muelhbauer, F.J. (1991). Allozyme and morphological variability, outcrossing rate and core collection formation in lentil germplasm. Theor. Appl. Genet. 83: 119-125.

  7. Ferguson, M.E. and Robertson, L.D. (1996). Genetic diversity and taxonomic relationships within the genus Lens as revealed by allozyme polymorphism. Euphytica 91: 163-172.

  8. Ferguson, M.E., Ford-Lloyd, B.V., Robertson, L.D. and Maxted, N. (1998). Mapping the geographical distribution of genetic variation in the genus Lens for the enhance conservation of plant genetic diversity. Mol. Ecol. 7: 1743-1755.

  9. Hamrick, J.L. and Godt M.J.W. (1997). Allozyme diversity in cultivated crops. Crop Sci. 37: 26-30.

  10. Jains, S., Porter, L.D., Kumar, A., Mir, R.R., Eigenbrode, S.D. and McPhee, K.E. (2014). Molecular and phenotypic characterization of variation related to pea enation mosaic virus resistance in lentil (Lens culinaris Medik.). Can. J. Plant Sci. 94: 1333-1344.

  11. Lázaro, A. and Aguinagalde, I. (2006). Genetic variation among Spanish pea landraces revealed by Inter Simple Sequences Repeat (ISSR) markers: its application to establish a core collection. J. Agr. Sci. 144: 53-62.

  12. Pérez-Vega, M. and García, P. (1997). Genetic structure of self-pollinating species: the case of wild Avena. Bocconea 7: 141-152.

  13. Pinkas, R., Zamir, D. and Ladizinsky, G. (1985). Allozyme divergence and evolution in the genus Lens. Plant Syst. Evol. 151: 131-140.

  14. Rodríguez, M.M., Paredes, M.C. and Becerra, V. (1999). Diversidad isoenzimática del germoplasma de lentejas (Lens culinaris Medik.) naturalizado en Chile. Agricultura Técnica 59: 186-195.

  15. Rosa, L. and Jouve, N. (1992). Genetic variation for isozyme genes and proteins in Spanish primitive cultivars and wild subspecies of Lens. Euphytica 59: 181-187.

  16. Selander, K., Smith, M.H., Yangw, S.Y., Johnson, E. and Gentry, J.B. (1971). Biochemical polymorphism and systematics in the genus Peromyscus. I. Variation in the old-fieldmouse (Peromyscus polionotus). Stud. Genet. 7103: 49-90.

  17. Sharma, K., Mishra, A.K. and Mishra, R.S. (2008). The genetic structure of taro, a comparision of RAPD and isozyme markers. Plant Biotechnol. Rep. 2: 191-198.

  18. Skibinski, D.O.F., Rasool, D. and Erskine, W. (1984). Aspartate aminotransferase allozyme variation in a germplasm collection of the domesticated lentil. Theor. Appl. Genet. 68: 441-448.

  19. SPSS (2006). SPSS 15.0 Brief Guide. SPSS Inc., Chicago.

  20. Tahir, M. and Muehlbauer, F.J. (1995). Association of quantitative trait loci with isozyme markers in lentil. J. Genet. Breed. 49: 145-150.

  21. Yeh, F., Yang, R. and Boyle, T. (1999). POPGENE version 1.31. University of Alberta, Edmonton.

  22. Zohary, D. and Hopf, M. (1994). Domestication of plants in the Old World. 2nd edition. Oxford University Press, Oxford.

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)