Phylogenetic study and analysis of similarities for different date palm cultivars using electrophoretic isoenzymes

DOI: 10.18805/ijare.v0iOF.10279    | Article Id: A-208 | Page : 232-237
Citation :- Phylogenetic study and analysis of similarities for different datepalm cultivars using electrophoretic isoenzymes .Indian Journal Of Agricultural Research.2016.(50):232-237

Adil M. Alissa*
Address :

Department of Basic Sciences, University of Dammam, Dammam, Saudi Arabia.

Submitted Date : 19-12-2015
Accepted Date : 25-02-2016


Non-specific esterases,  Glutamate Oxaloacetate Transaminase and Leucine-aminopeptidase  isoenzymes were extracted from fifteen date palm cultivars in The Al-Ahsa oasis in Saudi Arabia, and subjected to analysis by polyacrylamide gel electrophoresis. The results revealed differences in the degree of similarity between the cultivars from 0.147 to 0.552, estimated according to Jaccard‘s Similarity method on the basis of presence and absence of EST and GOT isoenzymes bands collectively.  Grr, Hel and Shl cultivars were closely related within a mini-cluster with degrees of similarity from 0.400 to 0.519. Ruz and Ows cultivars were closely related to each other with 0.552 degree of similarity. Tyr, OmR and Asi cultivars were closely related within a sub-cluster with degrees of similarity from  0.357 to 0.440. Mj and Khs cultivars were closely related to each other with 0.500 degree of similarity. It could be concluded that the isoenzymes are informative and helpful in the discrimination between the cultivars, as well as the results support the conclusion that most of  the The Al-Ahsa oasis date palm cultivars referred to one genetic origin. 


Band Date palm Dendrogram EST GOT Isoenzymes.


  1. Ahmed T, Al-Qaradawi A (2009). Molecular phylogeny of Qatari date palm genotypes using Simple Sequence Repeats Markers. Biotechnol 8: 126-131. 
  2. Al-Abbad A., Al-Jamal, M., Al-Elaiw, Z., Al-Shreed, F. and Belaifa, H. (2011). A study on the economic feasibility of date palm cultivation in the Al-Hassa Oasis of Saudi Arabia. J of Dev. and Agri. Econ. 3: 463-468.
  3. Al-Bakr A (1972). The Date Palm. Its Past and Present Status. Alani Press, Baghdad.
  4. Al-Fredan MA, (2013). Peroxidase activity in male and female plants of date palm (Phoenix dactylifera L.) Growing in Al-    Hassa, Saudi Arabia in Vitro , El-Minia Sci Bull, 24: 37–55. 
  5. Al-Helal AA (1992). Electrophoretic analysis of three selected Isoenzymes of date pollen grains, Bot Bull Acad Sinica 33:241-246. 
  6. AI-Helal AA (1985). The use of isoenzymes in the study of germination, development and breeding of legumes. Ph. D. thesis, University of Durham. 
  7. Al-Issa A.M. (2006). Echophysiological comparison between three cultivars of date palm in Al-Ahsa and Al-Qatif in Saudi Arabia, Ph. D thesis., King Saud Univ. Riyadh, Saudi Arabia.
  8. Al-Issa AM (2015). Electrophoretic analysis of proteins from different date palm (Phoenix dactylifera L.) cultivars in Saudi Arabia, AJB, 14:1325-1333. 
  9. Andy F (2009). Discovering statistics using SPSS , 3rd edition, SAGE Publications Ltd.
  10. Asif MI, Al-Tahir O and Al-Kahtani MS (1982). Inter-Regional and Inter-Cultivar Variations in Dates Grown in The KSA, Proc. of The First Symp. on The Date Palm, KFU, Al-Hassa, 1:234–48. 
  11. Attaha AHM, Mhoder TY and Abd AM (2013). Protein pattern of Phoenix dactylifera L. seeded strains grown in basrah region using cluster and principal component analysis. J plant sci 2(2).
  12. Barta J, Curn V and Divis J (2003). Study of biochemical variability of potato cultivars by soluble protein, isoesterase, and isoperoxidase electrophoretic patterns, Plant Soil Environ, 49: 230-236. 
  13. Davis, B. J. (1964) : Disc Electrophoresis. II. Method and Application to Human Serum Proteins . Ann. N Y Acad. Sci., 121:404–427. 
  14. El-Sherabasy SF, Wanas WH and Al-Kerdany AY (2008) Date palm cultivars in vitro screening to drought tolerance using isozymes, Arab J Boitech, 11: 263-72.
  15. Gottlieb LD (1977). Electrophoresis Evidence and Plant Systematic, Ann MO. Bot Gard 46:161-80 . 
  16. Haider N, Nubulsi I and Mir Ali N (2012). Phylogenetic relationships among date palm (Phoenix dactylifera L.) cultivars in Syria using RAPD and ISSR markers, J Plant Biol Res., 1: 12-24. 
  17. Hamrick JL and Godt RW (1990). Allozyme Diversity in Plant Species. In: Plant Population Genetics, Breeding, and Genetic Resaurces, AHD Brown, MT Clegg, AL Kahle, and BSWeir Sinuer Associates Editors, 43-63 . Sunderland, Mass.
  18. Hamza, H., M. Elbekkay, M. A. Ben Abederrahim & Ferchichi Ali A. (2011). Molecular and morphological analyses of date palm (Phoenix dactylifera L.) subpopulations in southern Tunisia. Spanish J of Agri. Res., 9: 484-493. 
  19. Khan A.S.M.M.R, Rabbani MA, Siddique MA and Isiam MA (2009). Studies on the Genetic Diversity of Pointed Gourd Using Biotechemical Methods (Isozyme Analysis), Bangladesh J. Agril. Res. 34: 123-141. 
  20. Kheirallah H, Al-Sammarraie SKI, Haider IM (2014). Molecular characterization of some iraqi date palms cultivars using RAPD and ISSR markers, J. Asian Sci. Res. 4:490-503.
  21. Ornestein, O. (1964) : Disc Electrophoresis – I. Background and Theory. Ann. N.Y. Acad. Sci. 12: 321-349 . 
  22. Ould Mohammad SA, Trifi M, Sakka H, Rhouma S and Marrakchi M (2001). Genetic inheritance analysis of four enzymes in date palm (Phoenix dactylifera L.). Genetic Resources and Crop Evaluation 48: 361-368. 
  23. Pathak K, Hatwal D, Bist R, Chandra D and Gaur AK (2011). Study of biochem. Variability in the populations of Acontum balfourii by soluble protein and isoenzyme electrophoretic patterns, J. Chem. Pharm. Res. 3: 295-301.
  24. Shawky AB and Hussain ST (2013). Complementary strategy for conservation of date palm germplasm. Global J Biodiv Sci Managem, 3: 96-107. 
  25. Stegemann H., Afify, A.E.M.R. and Hussein, K.R.F. (1987). Identification of date palm (Phoenix dactylifera L.) cultivars by protein patterns, Phytochemistry, 26:149-153.
  26. Zeidler M (2000). Electrophoretic analysis of plant Isoenzymes, Biol. 38:7-16. 

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