Background: Thuja [Tetraclinis articulata (Vahl) Masters], a native tree steeped in history, boasts a wealth of medicinal properties recognized across ancient global pharmacopoeias. 
 
Methods: The objectives of this study were to determine the volatile components in Tetraclinis articulata essential oils and examine their antibacterial properties in vitro. Gas chromatography-mass spectrometry (GC-MS) was employed to analyse the essential oil chemically. 
 
Result: GC-MS analysis revealed that the main components of the EO are bornyl acetate (26.25%), camphor (21.3%) and &alpha;-pinene (19.2%). The antimicrobial analysis revealed robust antibacterial and antifungal properties through the disc diffusion method, showing zones of inhibition greater than 15 mm. Gram-positive bacteria were found to have least MIC and MFC utilising the microdilution assay.

Characterization of Essential Oils of Tetraclinis articulata (Vahl) Masters from the Tiaret Region (Algeria) and Study of Their Antimicrobial Activity