Background: To evaluate the Platelet Storage Lesion (PSL) markers and extended storage time for SSP+ added Canine Platelet Concentrates (CPC) beyond five days at 22&deg;C.Fresh Canine CPCundergo certain metabolic and morphologic changes during storage. Estimation of PSL markers helps us better understand quality of the canine PC and to estimate the efficacy of SSP+ in increasing the life span of stored CPC.

Methods: CPC was prepared from 350 ml of whole blood, collected from 6 apparently healthy donor dogs by buffy coat methodin a quadruple bag closed system. CPC was divided into four aliquots of 15 ml each with one control and three test groups with SSP+ additive solution in different concentrations (65%, 75%, 85%). The&nbsp;In vitro PSL markers including swirling, pH, glucose, lactate, bicarbonate, pO2, pCO2, platelet concentration, MPV and PDWwere evaluated on day 1, 5, 9 and 13 of storage.

Result:&nbsp;The results were analysed by Two-way ANOVA wherein swirling, pH, glucose, lactate, bicarbonate, pO2, pCO2, platelet concentration, MPV showed significant difference (p&lt;0.01) between the groups. Interaction between the additive concentrations and the days of storage revealed a pH on day 9 for 65% SSP+ to be similar to day 5 for Plasma stored CPC while swirling, lactate and bicarbonate were also better maintained for 65% SSP+ PAS added CPC till day 9.Addition of 65% SSP+ PAS to CPC evidenced increased shelf life up to 9 days at 22&deg;C under agitation without significant deterioration in product quality.

Evaluation of Platelet Storage Lesion (PSL) Markers in SSP+ Platelet Additive Solution Added Canine Platelet Concentrates (CPC)

<div>Background: Impaction of colons is a major cause of deaths in equines; therefore the objective of the current study was to perform the detailed ultrasonographic examination of equids suffering from abdominal discomfort and to identify which measurable parameters were important in reaching a diagnosis of small intestinal abnormality and determining treatment options.&nbsp; 
 
Methods: In this study twenty five equids (age 10-13 years) with history of cessation of defecation ranging from 2-4 days were subjected to sonography.&nbsp; 
 
Result: The impaction of left ventral colon in all animals was imaged as hyperechoic intraluminal structure casting a strong acoustic shadow with loss of normal sacculations, edema, increase in wall thickness and absence of peristaltic activity. Findings giving an indication of sand colic were the flattening of left ventral colon wall against the ventral body wall with loss of normal sacculations, peristaltic activity, presence of small pinpoint hyperechoic structures on the mucosal surface seen casting small acoustic shadows and reverberation artifacts in different directions were highly suggestive of sand impaction. In all animals the loops of small intestine were observed in transverse as well as longitudinal sections as hypermotile distended with fluidy ingesta and hyperechoic feed particles swirling inside that fluidly ingesta. The presence of dilated multiple loops of intestine in a single scanning area was a consistent feature of left ventral colon impaction. Hence detailed ultrasonographic examination was useful to delineate normal and abnormal conditions and to distinguish between true and false colic which provided an important diagnostic lead in management of affections of left ventral colon.</div>

Evaluation of Platelet Storage Lesion (PSL) Markers in SSP+ Platelet Additive Solution Added Canine Platelet Concentrates (CPC)

Sonographic Evaluation of Coprostatic Impaction with Sand and Ingesta in Draft Equids