<div>Background:&nbsp;Dinh Lang [Polyscias fruticosa (L.) Harms] is a medicinal plant widely grown in Vietnam, with proven note-worthy health benefits. However, Dinh Lang&rsquo;s amounts of triterpenoid saponins could not meet the need of the pharmaceutical industry. Thus, this study&rsquo;s purpose is to figure out the optimal condition for raising Dinh Lang&rsquo;s cell biomass, rhizogenesis and somatic embryogenesis to provide materials for bioactive compound productions.&nbsp; 
 
Methods: Different 2,4-dichlorophenoxyacetic acid and &alpha;-naphthaleneacetic acid concentrations (0.5, 1.0, 1.5 and 2.0 mg/L) were examined to determine the best amount of each plant growth regulator for raising cells&rsquo; biomass, rhizogenesis and somatic embryogenesis. In each treatment, two grams of eight-week-old calli were cultured in 50 mL of liquid MS medium.&nbsp; 
 
Result:&nbsp;It is demonstrated by the results that liquid MS medium containing 1.5 mg/L &alpha;-naphthaleneacetic acid has the capacity of producing the highest numbers of somatic embryos (489 embryos per flask) and rooted cells (259.5 cells per flask), while the fresh weight of cells cultured in the medium given 1.5 mg/L 2,4-dichlorophenoxyacetic acid reached its peak of 5.7 g.</div>

The Effects of 2,4-Dichlorophenoxyacetic Acid and &alpha;-Naphthaleneacetic Acid on Biomass Increment, Rhizogenesis and Somatic Embryogenesis of Suspension-cultured Dinh Lang Cells [Polyscias fruticosa (L.) Harms]

<div>Background: Under prevailing regime of climate change and dynamic pest-crop interactions, greengram genetic improvement requires a speedier and precise tool such as genetic transformation to develop improved lines. In this context, an in vitro multiple shoot regeneration system via organogenesis was demonstrated in mungbean cv. SML668, a parent cultivar used in crop improvement programs, using double cotyledonary node (DCN) and detached embryonic axis (EA) explants with 1.0 mg l-1 BAP. 
&nbsp; 
Methods: While both the explants responded to in vitro regeneration, number of shoots regenerated was higher with EA (4.02&plusmn;0.19) than with DCN (3.1&plusmn;0.08). 6-benzyl aminopurine (BAP) was found to be most effective for inducing and regenerating multiple shoots, in comparison to all other phytohormones (NAA, IAA and TDZ) and supplements (amino acids) tested. Sub culturing twice on BAP supplemented media followed by two subcultures on basal media was optimal for multiple shoot regeneration. Rhizogenesis was obtained on basal media devoid of any phytohormones in EA explants and in 1.0 mg l-1 IAA for DCN explants. The in vitro regenerated plantlets were successfully hardened in a mixture of soil, sand and vermiculite that flowered, produced pods and viable seeds on maturity. 
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Result: The study revealed that detached embryonic axis was a potential explant for in vitro regeneration in SML668, a cultivar not tested for its in vitro regeneration ability before.&nbsp;&nbsp;</div>

The Effects of 2,4-Dichlorophenoxyacetic Acid and α-Naphthaleneacetic Acid on Biomass Increment, Rhizogenesis and Somatic Embryogenesis of Suspension-cultured Dinh Lang Cells [Polyscias fruticosa (L.) Harms]

Multiple Shoot Regeneration from Detached Embryonic Axis in Greengram (Vigna radiata) cv.SML 668