The development of an optimal culture medium for microbiological analysis is a significant advancement in the field. The conventional PCA for RAMF enumeration may not accurately assess raw camel milk&rsquo;s microflora, potentially compromising food safety evaluations. Our study addresses this crucial issue. We developed CaM-PCA medium using sterilized whole camel milk and compared it with standard PCA using twenty-three raw milk samples. Enumeration results were analyzed using Mann-Whitney test and exclusive isolates were identified through 16S rDNA sequencing. CaM-PCA yielded significantly higher RAMF counts (8.50&plusmn;0.45 log10 CFU ml-1) than PCA (8.20&plusmn;0.60 log10 CFU ml-1), P=0.048. Three isolates exclusive to CaM-PCA were identified as Metabacillus sp., Hafnia alvei and Enterobacter hormaechi subsp. hoffmannii, demonstrating CaM-PCA&rsquo;s enhanced ability to detect spoilage and opportunistic pathogens in raw camel milk.

Heat-stable Camel Milk-base Culture Medium Efficiency to Improve Microbiological Analysis of Raw Camel Milk

Background: In the Saharan ecosystems where the harsh environmental conditions persist, camels (Camelus dromedarius) with stand these conditions through their distinctive physiological adaptation and produce milk more than other animals over extended lactation period. The present study was designed to evaluate the effect of breeding system, milked she-camel population and the hygienic status of the udder on the physicochemical and microbiological proprieties of camel milk. 
 
Methods: 30 samples of camel milk were gathered during May and June 2024 in Tamanghasset (south Algeria), the samples originated from camels belonging to two populations and managed under two systems. After sanitary milking precautions, milk was subjected to california mastitis test (CMT), physicochemical and microbiological analysis. 
 
Result: The results of pH, acidity, conductivity, fat, solids non-fat (SNF), total solids, density, proteins, lactose, salt, freezing point and reviable aerobic mesophilic microflora (RAMF) analysis were 6.17&plusmn;0.27, 17.4&plusmn;3.05o&nbsp;D, 5.78&plusmn;0.86 mS/cm, 2.15&plusmn;0.94%, 9.03&plusmn;0.47%, 11.18&plusmn;1.09%, 31.80&plusmn;1.72, 3.28&plusmn;0.18%, 4.94&plusmn;0.21%, 0.69&plusmn;0.05%, -0.57&plusmn;0.03oC and 3.15&plusmn;1.03 UFC/ml respectively. Further analysis based on the T-test and Mann-Whitney exhibit a significant effect (p&lt;0.05) of breeding system on pH, acidity, conductivity and salt level; while only the bacterial load was significantly affected by camel population type, whereas the udder health status seems impact on the pH parameter. Complementing these findings, Principal component analysis (PCA) highlights an overall significant effect of udder health on milk composition and a moderate to slight influence of breeding system and camel population.

Heat-stable Camel Milk-base Culture Medium Efficiency to Improve Microbiological Analysis of Raw Camel Milk

Interplay of Management Practices and Physiological Factors Influences Camel Milk Characteristics in Tamanghasset, Southern Algeria