Background: Thymus daenensis is a phenol-rich species of the Thymus genus that possesses many biological and pharmaceutical activities.&nbsp; 
 
Methods: This study used five groups of TDLE extract (50, 100, 200 and mg/mL). Distilled water and mebendazole (10 mg/ml) were used as negative and positive controls. Five worms of similar size were placed in each Petri plate, together with the extract test solution (20 mL). The present work aims to study the phytochemical profiling, evaluation of anticancer properties and in vitro oocysticidal activity of methanolic T. daenensis leaves extracts (TDLE).&nbsp; 
 
Results: Infrared spectroscopy (FT-IR) of TDLE proved eleven different active classes of chemical compounds. The extract is characterized by higher phenolic contents (250.5&plusmn;2.7 mg/g of gallic acid) and flavonoids (25&plusmn;0.3 mg/g of quercetin). Also, TDLE showed moderate cytotoxicity against breast cancer cell lines (MCF-7) and lung cancer cell lines (A549) using MTT assay with LC50 attributed to 388.66&plusmn;3.5 &igrave;g/ml and 354.33&plusmn;2.5 &igrave;g/ml, respectively. The in-vitro study revealed that TDLE concentrations of 300 mg/ml caused significant suppression of Eimeria. papillata oocysts sporulation and increased percent of sporulation inhibition compared to the other commercial products which showed different levels of sporulation. Our findings indicate that TDLE has anticoccidial activity, which encourages the conduct of numerous In vivo investigations to find an effective treatment.

In vitro Sporulation, Oocysticidal Sporulation Inhibition of Eimeria papillate and Cytotoxic Efficacy of Methanolic Extract of Thymus daenensis Leaves

Background: Rabbit farming is important to boost wealth and increase protein intake. However, common parasitic infestations pose a threat to the survival Eimeria spp. The study aims to assess the effect of Calotropis procera leaves on Eimeria flavescens oocysts and determine the time and concentration to inhibit oocysts.&nbsp; 
 
Methods: Twenty-four-well plates with a volume of 3 mL, each containing one thousand un-sporulated oocysts/mL, were subjected to seven different treatments: Used was 2.5% potassium dichromate solution as the non-treated control; five extract concentrations (10, 20, 40, 80 and 100 mg/mL); In addition, 25 mg/ml toltrazuril as the reference medicine.&nbsp; 
The oocysticidal properties of the mixtures were assessed after 24, 48, 72 and 96 h.&nbsp; 
 
Result: The C. Procera extract was analyzed and quantitative results showed phenolics, flavonoids and tannins with different rates of bioactive compounds. Moreover, LC50 was obtained at 44.2&plusmn;0.007 g/mL for Hep-G2 cell lines. After 72 and 96 h, it was able to block the E. flavescens oocysts at 80% and 100% doses by approximately 81% and 89%, respectively. The relationship between sporulation and concentration was highly negative at all times. Results provide scientific validation for the application of C. procera extracts in the prevention of coccidiosis.

Evaluating in vitro Efficiency of Calotropis procera against Eimeria flavescens Oocysts, Isolated from Domestic Rabbits