<div style="text-align: justify;">Plant biomass is the basis for the calculation of net primary production. Estimation of fresh biomass in high throughput way is critical for plant phenotyping. Conventional phenotyping approaches for measuring the fresh biomass is time consuming, laborious and destructive in nature. Image analysis based plant phenotyping is very popular nowadays. Most of the approaches used projected shoot area from visual images (VIS) to estimate the fresh biomass. As water content has a significant effect on fresh biomass and water absorbs radiation at near infra-red (NIR) region (900nm to 1700nm), we have hypothesized that the combined use of VIS and NIR imaging can predict the fresh biomass more accurately that the VIS image alone. In this study, VIS and NIR images were collected using LemaTec facility installed at Nanaji Deshmukh Plant Phenomics Center, ICAR-IARI, New Delhi-12. In this study, VIS and NIR imaging were captured for rice leaves with different moisture content as a test case. MATLAB software (version 2015b) was used for image analysis. The two image derived parameter viz. Green Leaf Proportion (GPR) from VIS image and mean gray value/intensity (MGV_NIR) from NIR image were used to develop the statistical model to &nbsp;estimate the fresh biomass in the form of Leaf Fresh Weight (LFW). The proposed approach significantly enhanced the fresh biomass estimation.</div>

Plant biomass estimation using image analysis and machine learning technique