Oestrus ovis L. (O. ovis) (Diptera: Oestridae) (Sheep Bot Fly) is ubiquitous in distribution. Myiasis causing larvae collected from Abu-Arish area (Eastern Jazan), Saudi Arabia were confirmed as O. ovis based on morphological traits. Polymerase chain reaction (PCR) studies targeting amplification of partial fragment (606 bp) of mitochondrial cytochrome oxidase subunitI (mtCOI) gene further confirmed the species. Phylogenetic analysis based on the partial (mtCOI) gene sequence revealed that the accession KU921431 showed 97% similarity based on nucleotide pairs of O.ovis accessions, retrieved from the GenBank. Thus, a method of molecular identification of O.ovis larvae was established as a credible substitution to morphological identification in Jazan region, Saudi Arabia.

Molecular identification studies &nbsp;on Oestrus ovis L. (Diptera:Oestridae) larvae infested sheep in jazan region , Saudi Arabia

Background: Malaria can infect erythrocytes and hence cause different pathogenesis episodes leading to death mostly in pregnant women and children under the age of 5 years. The selective pressure of these parasites leads to the production of new human genetic diseases. The most prevalent genetic alterations in the human genome are thalassemia and hemoglobinopathies (Hb E, Hb S), which are recognized throughout the world, including Saudi Arabia.&nbsp; 
 
Methods: From May 2018 to August 2019, 13972 Saudi citizens from King Fahd Central Hospital and premarital facilities in the Saudi Arabian province of Jazan participated in this study. This study aims to compare the prevalence of Hb E and other hemoglobinopathies in positive versus negative cases of malaria. So, CBC, malaria test, Hb-electrophoresis, and molecular study were investigated.&nbsp; 
 
Result: For thalassemias and Hb disorders, 36% with abnormal Hb (47% of them) carried Hb S in their blood, 37% with &alpha;-thalassemia, 11% for &beta;-thalassemia and 4% of Hb E. Significant variations in CBC parameters were observed in Hb E patients. There was significant decrease in MCV, MCH and MCHC and slightly increase in WBCs, RBCs, RDW and PLT as compared to controls.

Molecular identification studies on Oestrus ovis L. (Diptera:Oestridae) larvae infested sheep in jazan region , Saudi Arabia

Hemoglobin E Prevalence among People Residing in Malaria Areas