<div>Diarrhoeal disease is considered a public health problem with high morbidity and mortality worldwide, especially among children under the age of five years. In India, like other developing countries, diarrhoeal diseases are important child health problem. Food is an important factor in transmitting pathogens that cause diarrheal illness. Diarrhea can be caused by numerous pathogens and transmitted through multiple vehicles. Complementary foods prepared under unhygienic conditions are frequently heavily contaminated with pathogens and may thus be a major factor in causing diarrhoea diseases. Diarrheal diseases are major causes of malnutrition, delayed physical development and early childhood mortality. Diarrhea can cause acute wasting and is the most important infectious determinant of stunting of children&rsquo;s linear growth. The prevention of diarrhoea in infants and children requires a multidisciplinary approach, including the promotion of safe preparation and handling of complementary food. Food contamination can be reduced using a Hazard Analysis and Critical Control Point (HACCP) methodology at the household level. Mothers and caregivers must wash their hands before feeding children and preparing food.&nbsp;</div>


<p>​Burden of Diarrhoeal Diseases through Complementary Foods: A Review</p>


<p style="text-align: justify;">Present investigation was carried out to study the bacterial load of different semen extender in frozen thawed semen samples of Sahiwal bulls maintained at Artificial Breeding Research Centre, ICAR-NDRI, Karnal, Haryana. Twenty four ejaculates from four bulls were collected during morning hours using sterilized artificial vagina and the ejaculates were evaluated. Semen samples showing more than 70 per cent progressive motility and having more than 800 million sperms per ml were used for further processing and freezing. The samples were split into six aliquots and extended in six different extenders [Conventional egg yolk (CEY), egg yolk of hen supplemented with omega enriched diet (group I), Egg yolk of hen supplemented with herbal feed (group II), 1% soya lecithin (SY1), commercially available Bioxcell (SY2) and Optixcell (LP)]. One ml of post thaw semen (four straws) was serially diluted (ten-fold) with normal saline, loaded in sterilized nutrient agar plate and incubated at 37&deg;C for 48 hours. Number of colonies were counted in duplicate and multiplied by dilution factor. The Standard plate counts (CFU/ml) of frozen thawed semen samples extended in different extenders were analyzed by one way analysis of variance. The results revealed that bacterial load of SY1, SY2 and LP was significantly (p&lt;0.05) different from egg yolk based extenders. The highest microbial load of frozen thawed semen samples was recorded in group II (646.30&plusmn;71.65) egg yolk extender and lowest in optixcell (91.67&plusmn;6.72) extender. Microbial load in all the extenders are well within the standard limit (5000CFU/ml) of frozen thawed semen sample. Lower bacterial load in present finding may be due to maintenance of better HACCP protocol for hygiene during preparation, semen collection and semen processing.</p>


<p>Microbial load of frozen thawed Sahiwal semen extended in egg yolk, soya lecithin and liposome based extender&nbsp;</p>


<div style="text-align:justify"><strong>Background:</strong> Shiga toxin-producing Escherichia coli (STEC) strains are considered to be most common food-borne enteric zoonotic pathogen, causing various disease conditions in both animals and humans and are highly pathogenic to human in low infectious doses. Resistance against antibiotics by STEC is also a big concern now a days. Hence in view of the public health significance of STEC, present work was planned to know the combination of phenotypic and genotypic resistance patterns against certain most commonly used antibiotics by using disk diffusion method and multiplex PCR respectively.<br />
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<strong>Methods:</strong> A total of 426 PCR confirmed STEC isolates isolated from pooled samples (animal faecal(179), farm water(122) and human faecal samples (125) of different livestock farms in and around Proddatur andhra Pradesh, were subjected to antibiotic sensitivity test by using disc diffusion method. The isolates that showed resistance for tetracycline, streptomycin, sulphonamides and ampicillin were selected and subjected to multiplex PCR for the detection of resistance genes.<br />
<br />
<strong>Result:</strong> Disk diffusion assay revealed highest phenotypic resistance for STEC isolates against Cepahlothin (100%), followed by Ampicillin (99.06%), Tetracycline (97.2%), Streptomycin (94.3%), Sulphonamides (90.8%) and Trimethoprim (84.5%). Pooled samples also revealed the presence of antimicrobial resistance genes like tetA(59.2%), tetB(43.5%), tetC (9.2%), strA (39.3%), strB(54.1%), sul1 (40.8%), sul2 (58.7%), sul3 (3.8%) and blaTEM(83.4%). These findings indicate the highest prevalence of antimicrobial resistance among the STEC isolates, which alarms indiscriminate use of antibiotics both for therapeutic purpose and as growth promoters. Strict hygienic, sanitation and HACCP programmes should be applied to counter STEC prevalence.</div>

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​Burden of Diarrhoeal Diseases through Complementary Foods: A Review

Microbial load of frozen thawed Sahiwal semen extended in egg yolk, soya lecithin and liposome based extender

Detection of Antimicrobial Resistance Genes from Shiga Toxin Producing Escherichia coli by Multiplex PCR

Burden of Diarrhoeal Diseases through Complementary Foods: A Review