<div>Background: The present investigation was carried out with the objective of optimization of rapid micropropagation protocol and to evaluate effect of different concentrations of plant growth regulators added singly or in combinations on in vitro culture of Gliricidia sepium.&nbsp; 
 
Methods: Explants (Soft nodal stem segments) were inoculated on MS medium containing varying concentrations of cytokinins and auxins either singly or in combinations. The cultures were incubated at 25&plusmn;2&deg;C for 14: 10 hour&rsquo;s photoperiod with a light intensity of 3000-3500 lux.&nbsp; 
 
Result: Maximum number of shoot bud induction obtained when MS medium was supplemented with 0.5 mg/l BAP with 100 per cent frequency. Highest frequency (100%) of rooting was observed at 0.5 mg/l of IAA. 2.0 mg/l NAA showed highest number of roots/explant but the roots were thick. For shoot bud induction, BAP was found to be most effective among all the plant growth regulators. For root induction, both NAA and IAA were found to be effective but IAA exhibited higher frequency of rooting as compared to NAA.</div>

Effect of Plant Growth Regulators on in vitro Morphogenic Response of Gliricidia [Gliricidia sepium (Jacq.) Steud.]

An experiment was conducted during 2010 &ndash; 2012 to find out the suitable lopping interval of gliricidia (Gliricidia sepium) on sandy loam soil. There were six treatments viz., T1: lopping once in 2 months, T2: lopping once in 4 months, T3: lopping once in 6 months, T4: lopping once in 8 months, T5: lopping once in 10 months and T6: lopping in 12 months and replicated thrice. The results showed that lopping at shorter time interval i.e once in 2 months or 4 months resulted in more number of branches, leaves, leaf weight, leaf branch ratio and increased yield. Further, early lopping resulted in succulent leaves and branches which are highly suitable for the animals to consume.&nbsp;

Effect of lopping interval on the growth and fodder yield of Gliricidia sepium 
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<div style="text-align: justify;">The aim of this study was to evaluate the effect of soil cover with a combination of tree leguminous on increasing grain yield, grain protein content, and maize protein yield. The experiment was conducted with 10 treatments: Leucaena leucocephala + Clitoria fairchildiana + urea (L+C+U); L. leucocephala + Acacia mangium + urea (L+A+U); Gliricidia sepium + C. fairchildiana + urea (G+C+U); G. sepium + A. mangium + urea (G+A+U); L. leucocephala + C. fairchildiana (L+C); L. leucocephala + A. mangium (L+A); G. sepium + C. fairchildiana (G+C); G. sepium + A. mangium (G+A); bare soil with urea (B+U); and a control with bare soil without mineral fertiliser. Differences between G+C+U and B+U, in the agronomic efficiencies, increase in N contents, increase in grain yield. To increase the protein yields in humid regions, leguminous are used to provide nutrients and improve the root environment rather than interacting antagonistically with the crops.</div>

Effect of Plant Growth Regulators on in vitro Morphogenic Response of Gliricidia [Gliricidia sepium (Jacq.) Steud.]

Effect of lopping interval on the growth and fodder yield of Gliricidia sepium

How leguminous biomass can increase yield and quality of maize grain in tropical agrosystems