<div>Background: Trypanosomiasis, an arthropod-borne blood protozoan disease commonly known as Surra, has an adverse influence on the health and working capability of infected animals. Monitoring and identification of blood-borne parasitic infections in dairy animals are of vital importance to get the optimum production. There was a lake of the scientific report of incidence of trypanosomiasis in buffalo in the northeast region.&nbsp;&nbsp; 
 
Methods: In the present study, a total of 100 buffalo was screened in the Deepor Beel area of Assam and 25 animals were suspected of trypanosomiasis based on clinical signs. The diagnosis was confirmed by microscopic examination and formol gel slide test which was further confirmed by PCR. The blood from twenty-one buffalo tested positive for the presence of Trypanosoma evansi was evaluated for clinico-haemato-biochemical alterations.&nbsp; 
 
Result: The outbreak in the affected animals exhibiting high fever (105&plusmn;1.0&deg;F), hyperemic mucosa of eyes, corneal opacity, incoordination of the hindquarter, wasting condition of body and death, was confirmed based on examination of Wright-Giemsa stained blood smears, formol gel slide test and by polymerase chain reaction by using T. evansi specific primers yielding species-specific 227 bp PCR product. The infected bovines showed marked anaemic tendency as revealed by a significant decrease in Hb, PCV, RBC, Lymphocytopenia and leukocytosis along with marked thrombocytopenia. There is a marked increase in BUN, creatinine and total bilirubin level while random blood sugar shows a decreased level.</div>

Clinico-hematological and Biochemical Studies on Naturally Infected Buffaloes with Trypanosomiasis in Deepor Beel Area of Assam