<div>Exposure of C6 and CHO-K1 cells to different concentrations of the antineoplastic drug paclitaxel resulted in a loss of cellular viability. The percentage of surviving cells fell significantly after 48 hours of treatment and IC50 values observed were between 0.5 to 0.75 and 0.25 to 0.75 &micro;g/ml in C6 and CHO-K1 cells, respectively. No significant cytotoxicity was observed after 24 hours of treatment and cells incubated at higher concentrations of paclitaxel showed increased survivability. Paclitaxel induced apoptosis by caspase 3/7 activation and caused accumulation of cells in the G2/M phase of the cell cycle. Upon fluorescent microscopy, both the cell lines lost the morphology, confluence and adherence at 24 hours but effects were much more pronounced at 48 hours of treatment. The in vitro data suggested that paclitaxel is highly effective when there is prolonged exposure of tumor to the drug rather than increasing the intratumoral or biophasic concentration of the drug.&nbsp;</div>

<div>In vitro Cytotoxicity, Apoptosis, Effects on Cell Cycle Kinetics and Schedule-Dependent Effects Induced by Paclitaxel on C6 and CHO-K1 Cell Lines</div>

Background: Production of pulses is a crucial need of the country because the India is important producer of pulses even though we are deficient in daily protein consumption. Encouraging of redgram intercropping in cotton is a fruitful way to increase the area of redgram and productivity along with income of cotton-redgram farmers. 
 
Methods: Study conducted by DAMU (District Agromet Unit), Krishi Vigyan Kendra, Mancherial district to understand the impact of Agromet Advisory Service on yield and economics of Cotton- Redgram intercropping system during 2019-20 to 2021-22 in 5 locations of Mancherial and K.B. Asifabad districts. To understand the impact of Advisory service, encouraged the farmers to practice cotton-redgram inter cropping system over traditional sole cotton cropping to increase the crop yield and economics in the study area. 
 
Result: Results of 5 locations revealed that the farmers followed and practiced cotton- redgram intercropping along with suggested management practices according to the Agromet advisory service experienced superior yields (Three years average of 5 locations inter cropping yield was 2031 kg ha-1&nbsp;including cotton 1537 kg ha-1&nbsp;and red gram 494 kg ha-1) in all the three years compared to sole cotton crop (Three years average of 5 locations 1690 kg ha-1). Luxury yields of intercropping through the AAS service also showed positive impact on LER and CER. These effective utilization of resources through LER and profitability through the CER leads to better gross, net income as well as B:C ratio in intercropping.

In vitro Cytotoxicity, Apoptosis, Effects on Cell Cycle Kinetics and Schedule-Dependent Effects Induced by Paclitaxel on C6 and CHO-K1 Cell Lines

Impact of Agromet Advisory Service on Yield and Economics of Cotton-Redgram Intercropping System