Background: Root-knot nematodes are considered the most prevalent pathogens worldwide due to their extensive host range, which includes approximately 200 hosts. 
 
Methods: This study evaluated the impact of two nematicides, fluopyram and cyclobutrifluram, at a concentration of 400 ppm/liter of water against Meloidogyne incognita under greenhouse conditions during the spring season of 2025. Treatments were made every four weeks over two intervals. M. incognita was identified through morphological and molecular diagnosis. 
 
Result: The adult female&rsquo;s perineal patterns and molecular diagnosis of RKN isolates identified as Meloidogyne incognita. The sequencing of the M. incognita was deposited at the GenBank under the accession number (PQ600915.1). The most effective application was fluopyram and cyclobutrifluram, which applied by hand injector, showed such a significant impact against gall index, the quantity of juveniles (J2) after application, the percent reduction of juveniles (J2), eggs, females, final population and reproduction factor with values of 0.33, 0.67, 31.67, 49.00, 98.48%, 97.58%, 108.333, 346.00, 0.667, 1.000, 140.667, 396.000 and 0.132, 0.381, respectively, as well as increased plant height (cm), fresh shoot weight (g), dry shoot weight (g), yield per plant (kg/m&sup2;), root length (cm) and fresh root weight (g) with values of 364.33, 331.67, 416.67, 393.67, 106.33, 99.67, 13.45, 12.45, 33.667, 29.667 and 17.103, 16.287, respectively, compared to the control treatment.&nbsp; 
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Evaluation of the Efficacy of Fluopyram and Cyclobutrifluram against Meloidogyne incognita on Cucumber under Greenhouse Conditions