Background: Fluoroquinolone antibacterials are considered as the most beneficial antibacterial agents due to their high activity against various types of bacteria. Besifloxacin (Besi), is a novel fluoroquinolone antibacterial agent with a broad spectrum of activity. Besi demonstrates potent antibacterial activity&nbsp;in vitro&nbsp;against a wide range of pathogens; it has also shown high potency against a lot of infectious pathogens and other ocular and systemic infections. Less data are demonstrated in the literature on the tolerability of Besi novel metal drug complexes. The main objective of the current study is the treatment of hepatotoxicity and the oxidative stress damage via using novel metal drug complex in male rats. 
 
Methods: Novel Manganese besifloxacin complex was synthesized. The ability of besifloxacin (Besi) to form chelates with divalent manganese was investigated. Confirmation the structures of the complex has been elucidated by CHN analysis, molar conductance, IR, UV-Vis spectral studies, magnetic moment, scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray powder diffraction (XRD). The data suggested that Besi form a 1:2 chelates with Mn (II) ion. The spectral data confirm that Besi act as bidentate via C=O and deprotonated COOH group. Eighteen male albino rats (6 Rats/group) were separated randomly into 3 treated groups. Control, Besi (100 mg/kg) and Besi/Mn (100 mg/kg) at the same dosages. At the end of the experiment, liver and blood samples were analyzed for biochemical, molecular and histological variations. 
 
Result: Result revealing that Besi administration significantly elevated the levels of the liver enzymes as a result of Besi administration contrary to Besi/Mn administration. Histopathological examinations revealed inflammatory liver areas. Furthermore, induction of significant increases in NF-&kappa;B, JNK and CHOP as compared to the normal control group. Meanwhile, induction of a significant decline in NRF-2 level of Endoplasmic Reticulum oxidative damage, which was detected in the hepatocytes. In contrast to Besi/Mn novel complex treatment, significant hepatic amelioration was observed and a decline in hepatocellular congestion and oxidative stress genes after its administration. Besi/Mn complex was found to be with high antimicrobial potency.

Besifloxacin/Mn Novel Metal Complex Ameliorates Hepatic Alterations in Male Rats via Modulating NRF-2 Antioxidant Hepatic Signalling Metabolism and NF-&kappa;B/ JNK and CHOP Oxidative Stress Pathway