<div style="text-align:justify">Background: Cassava is a vital food security crop for millions of people, especially in sub-Saharan Africa. Since the crop produces a reasonable yield on marginal soils, it could help alleviate world hunger. Consequently, the increase in cassava production and its quality characteristics are significant. However, the low multiplication rate of this main crop has delayed the spread of improved varieties among farmers. As a result, tissue culture techniques can be a viable solution to overcome these challenges. 
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Methods: The study used a nodal segment as an explant to evaluate different concentrations of BAP and NAA for an efficient, cost-effective in vitro mass multiplication of the AWC-1 cassava variety. CuSO4, commercial bleach and ethanol had used to sterilize nodal explants taken from greenhouse-grown plants at various time intervals. 
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Result: The best medium for micro shoots induction had found to be medium without growth regulators. Among different treatments used for shoot multiplication purposes, the maximum shoot number has been recorded on M.S. medium supplemented with 0.75 mg/l BAP and 0.2 mg/l NAA. Medium with 0.5 mg/l NAA concentration was the best for rooting induction. A survival rate of 86% has obtained in the greenhouse and the plantlets appeared to be morphologically normal.</div>

Protocol Optimization for Rapid Multiplication of Cassava (Manihot esculenta Crantz) using the Nodal Segment