<div>Background: Gastro-intestinal anastomosis is mostly performed to correct the gastro-intestinal surgical disorders like gastro-intestinal obstruction, abdominal trauma, perforated bowel, intussusception and intestinal neoplasia etc. In early periods conventional intestinal anastomosis was followed which evolved into a seromuscular pattern of suture technique in recent years. Stapled anastomosis have a higher tensile strength than sutured anastomosis, since they heal by primary intention with minimal inflammation. Taking above facts into consideration, this study was undertaken to compare the conventional suture with stapler anastomosis of gastro-intestinal surgery in dogs. 
 
Methods: Clinical cases of dogs which were reported to Small Animal Out-Patient (Surgery Unit) of Madras Veterinary Teaching Hospital, Chennai, with the symptoms of gastro-intestinal obstruction due to multifactorial causes formed the study material that were randomly divided into two groups of 6 dogs each. Group I Cases were treated with conventional suture, Group II were treated with gastrointestional anastomosis staplers. Plain and contrast radiography, ultrasonography, intestional anastomosis time, resumption of oral feeding, matrix metalloproteinase were performed. 
 
Result: Stapler technique was found to be superior, accurate and most effective than the conventional technique in terms of anastomotic constructing time, less bowel manipulation, tissue trauma, anaesthetic exposure, risk of peritoneal contamination, early resumption of gastro-intestinal motility and oral feeding, rapid healing at the anastomotic site and&nbsp; reduced hospital stay. Moreover the procedure was easy to perform and simple.&nbsp;</div>

A Comparative Study of Conventional Suture and Stapler Anastomosis for Gastro-intestinal Surgery in Dogs

<div>Background: Mungbean (Vigna radiata&nbsp;L. Wilzeck) is one of the most important pulse crops and grown in almost all parts of the India. Web blight/wet root rot disease of mungbean is caused by Rhizoctonia solani K&uuml;hn. Crop environmental factors plays a vital role in the development of web blight disease caused by R. solani. An understanding of the role of environmental factors on the infection and survival of the pathogen is necessary to develop disease management practices. 
 
Methods: The effect of different temperatures (4oC, 20oC, 25oC, 30oC and 35oC) on mycelial growth of seven different R. solani isolates belonging to different anastomosis group were evaluated under in vitro conditions. Effect of different temperatures on the development of root rot/web blight disease of mungbean was also evaluated under phytotron conditions at various temperatures with constant relative humidity (85%) and illumination (alternate dark and light period of 12 h). Effect of temperatures on the expression of selected pathogenicity related genes was evaluated through real time PCR. 
 
Result: Maximum radial growth in R. solani isolates was observed at 25 and 30oC after 48 hrs of incubation. Maximum disease incidence was observed with R. solani isolate RUPU-18 (73.11%) followed by R-17 (68.75%), RDLM-1 (63.45%) at 25oC on mungbean genotype Pusa Vishal. Expression of genes like ABC transporter was observed only at 35oC, while other genes like 1, 3 glucan hydrolase expressed maximum at 25oC after 24, 48 and 72 hrs post inoculation. Present study suggested that the expression of pathogenicity related genes in mungbean-R. solani system is dependent on the temperature and time interval post pathogen inoculation.</div>

<div>Influence of Temperature on the Pathogenesis and Gene Expression of Rhizoctonia solani Causing Web Blight/Wet Root Rot Disease in Mungbean</div>