Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 44 issue 12 (december 2021) : 1521-1528

Impacts of Rhizobium Strain Ar02 on the Nodulation, Growth, Nitrogen (N2) Fixation Rate and ion Accumulation in Phaseolus vulgaris L. under Salt Stress

Saoussen Kouki, Boulbaba L’taief, Rahamh N. Al-Qthanin, Bouaziz Sifi
1Department of Biology, College of Sciences in Abha, King Khaled University, P.O. Box 960, Abha, Saudi Arabia.
Cite article:- Kouki Saoussen, L’taief Boulbaba, Al-Qthanin N. Rahamh, Sifi Bouaziz (2021). Impacts of Rhizobium Strain Ar02 on the Nodulation, Growth, Nitrogen (N2) Fixation Rate and ion Accumulation in Phaseolus vulgaris L. under Salt Stress. Legume Research. 44(12): 1521-1528. doi: 10.18805/LRF-4716.

Background: Phaseolus vulgaris L. -rhizobia symbiosis has effectively enhanced common bean productivity via multiple biological mechanisms. This study aims to assess the impacts of the strain of Rhizobium on the nodulation, growth, nitrogen (N2) fixation rate and ion accumulation within Phaseolus vulgaris L. under salt stress.
Methods: The Coco Blanc cultivar of the common bean was inoculated with the Ar02 rhizobia strain at 15 days after germination. Bean plants were inoculated in perlite culture to which salt was added in concentrations of 0, 25, 50 and 75 mmol L-1 NaCl. 
Result: Inoculation with the Ar02 rhizobia strain led to infective and effective symbiosis with the common bean plants exposed to saline solutions and non-saline solutions, respectively. Nodule biomass and nitrogen content declined under salt stress but maintained a higher number of nodules and nodule biomass at 75 mM NaCl. Plant root and shoot length increased with higher biomass under saline conditions, significantly more than the non-inoculated plant without salt. However, the progressive addition of NaCl reduced the growth of the root and shoot and the biomass within the inoculated plant. Salinity led to increased Na+ within the plant’s shoot, along with a reduction in Ca+2 and K+ concentrations. The shoot’s Ca+2, Na+ and K+ content were higher in the inoculated plant than the non-inoculated. The salt tolerance in common bean plants inoculated with Ar02 rhizobia was linked with the plant’s capability to sustain nodulation and enhance Na+ concentration in the shoot. Furthermore, salt tolerance within the same variety inoculated with Rhizobium was linked to a decline in the Ca+ and K+ concentrations in the shoot region of salt-exposed plants.

  1. Ahmad, M., Zahir, Z.A., Asghar, H.N. (2011). Inducing salt tolerance in mung bean through coinoculation with rhizobia and plant growth promoting rhizobacteria containing 1-aminocyclopropane 1- carboxylate deaminase. Canadian Journal. Microbiology. 57: 578-589.

  2. Attia, H., Karray, N., Rabhif, M., Lachaal, M. (2008). Salt-imposed restrictions on the uptake of macroelements by roots of Arabidopsis thaliana. Acta Physiologiae Plantarum. 30: 723-727.

  3. Benidire, L., Lahrouni, M., El Khalloufi, F., Gottfert, M., Oufdou, K. (2017). Effects of Rhizobium leguminosarum inoculation on growth, nitrogen uptake and mineral assimilation in Vicia faba plants under salinity stress. Agricultural Science and Technology. 19: 889-901.

  4. Ben Khaled, L., Morte Gomez, A., Honrubia, M. Orhabia, A. (2003). Effet du stress salin en milieu hydroponique sur le Rhizobium. Agronomie. 23: 553-560.

  5. Bhardwaj, K.K.R. (1975). Survival and symbiotic characteristics of Rhizobium in saline-alkali soils. Plant Soil. 43: 377-385.

  6. Borsani, O., Cuartero, J., Fernandez, J. A., Valpuesta, V., Botella, M.A. (2001). Identification of two loci in tomato reveals distinct mechanisms for salt tolerance. Plant Cell. 13: 873-887.

  7. Chen, Z., Newman, I., Zhou, M., Mendham, N., Zhang, G., Shabala, S. (2005). Screening plants for salt tolerance by measuring K+ flux: A case study for barley. Plant Cell and Environment. 28: 1230-1246. 

  8. Dutta, P. and Bera, A.K. (2014). Effect of NaCl salinity on seed germination and seedling growth of Mung bean cultivars. Legume Research. 37(2): 161-164.

  9. El-Akhal, M.R., Rinco´n, A., Coba de la Pen˜a, T. (2013). Effects of salt stress and rhizobial inoculation on growth and nitrogen fixation of three peanut cultivars. Plant Biology. 15: 415-421.

  10. Elsheikh, E.A.E. and Wood, M. (1990). Effect of salinity on growth, nodulation and nitrogen yield of chickpea (Cicer arietinum L.). Journal of Experimental Botany. 41: 1263-1269.

  11. Grattan, S.R. and Maas, E.V. (1988). Effect of salinity on phosphate accumulation and injury in soybean: 1. Influence of CaCl2/ NaCl ratios. Plant Soil. 105: 25-32.

  12. Gulmezoglu, N., Aydogan, C., Turhan, E. (2016). Physiological, biochemical and mineral dimensions of green bean genotypes depending on Zn priming and salinity. Legume Research. 39(5): 713-721.

  13. Hafeez, F.Y., Aslam, Z., Malik, K.A. (1988). Effect of salinity and inoculation on growth, nitrogen fixation and nutrient uptake of Vigna radiata L. Wilczek. Plant Soil. 106: 3- 8.

  14. Hu, Y., Fricke, W., Schmidhalter, U. (2005). Salinity and the growth of non-halophytic grass leaves: the role of mineral nutrient distribution. Functional Plant Biology. 32: 973-985.

  15. Khan, F. (2018). Salinity stress phenotyping for soybean (Glycine max L.) for Middle East Asia. Legume Research. 41(4): 551-556.

  16. Khan, M.A., Ungar, I.A., Showalter, A.M. (2000). Effects of salinity on growth, water relations and ion accumulation of the subtropical perennial halophyte, Atriplex griffithii var. stocksii. Annals of Botany. 85: 225-232.

  17. L’taief, B., Abdi, N., Smari, S., Ayari-Akkari, A., Jeridi, M., Alsenidi, M., Sifi, B. (2020). Effects of Rhizobium strain on the growth, nodulation, N2 fixation and ions accumulation in Vicia faba plant under salt stress. Legume Research. 43(4): 573-579. 

  18. Mohammad, M., Campbell, E.F., Rumbaugh, M.D. (1989). Variation in salt tolerance of alfalfa. Arid Soil Reabil. 3: 11-20.

  19. Morgan, R.S., Abd El-Hady, M., Rahim, I.S. (2018). Soil salinity mapping utilizing sentinel-2 and neural networks. Indian Journal of Agricultural Research. 52: 524-529.

  20. Rosas, S.B., Avanzini, G., Carlier, E., Pasluosta, C., Pastor, N., Rovera, M. (2009). Root colonization and growth promotion of wheat and maize by Pseudomonas aurantiaca SR1. Soil Biology and Biochemistry. 41: 1802-1806.

  21. Singleton, P.W. (1983). A split-root growth system for evaluating the effect of salinity on the components of the soybean Rhizobium japonicum symbiosis. Crop Science. 23: 259-262.

  22. Singleton, P.W., Swaify, S.A., Bohlool, B.B. (1982). Effect of salinity on Rhizobium growth and survival. Applied and Environmental Microbiology. 44: 884-890.

  23. Ushakova, S.A., Kovaleva, N.P., Gribovskaya, I.V., Dolgushev, V.A., Tikhomirova, N.A. (2005). Effect of NaCl concentration on productivity and mineral composition of Salicornia europaea as a potential crop for utilization NaCl in LSS. Advances in Space Research. 36: 1349-1353.

  24. Vincent, J.M. (1970). A Manual for the Practical Study of Root- Nodule Bacteria, I.B.P. Handbook no. 15, p. 44. Oxford: Blackwell Scientific Publications.

  25. Waisel, Y. (1989). Adaptation to Salinity. In: Physiology of Stress. [Raghavendra, A.S. (ed.)], John Wiley and Sons, New York p. 359-383.

  26. Weil, R.R. and Khalil, N.A. (1986). Salinity tolerance of winged beans as compared to that of soybean. Agronomy Journal. 78: 67-70.

  27. Yousef, A.N. and Sprent, J.I. (1983). Effect of NaCl on growth, nitrogen incroporation and chemical composition of inoculated and NH4NO3 fertilized Vicia faba L. plants. Journal of Experimental Botany. 143: 941-950.

Editorial Board

View all (0)