Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.80

  • SJR 0.391

  • Impact Factor 0.8 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Legume Research, volume 44 issue 8 (august 2021) : 888-893

Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers

Mehmet Macit Ertuş
1Department of Plant and Animal Production, Colemerik Vocational School, Hakkari University, Turkey.
  • Submitted04-02-2021|

  • Accepted22-05-2021|

  • First Online 27-07-2021|

  • doi 10.18805/LR-614

Cite article:- Ertuş Macit Mehmet (2021). Genetic Relationship in Sainfoin (Onobrychis viciifolia) Landraces Cultivated East Anatolia by using RAPD and ISSR Markers. Legume Research. 44(8): 888-893. doi: 10.18805/LR-614.
Background: Sainfoin (Onobrychis viciifolia) is a forage crop that yields high in arid and calcareous soils and is cultivated in large areas. There aren’t many genetic diversity studies on the varieties of cultured sainfoin. This study was conducted to determine the genetic diversity and the degree of relationship between 23 cultivated landraces and one registered variety. 
Methods: To take samples from the populations, seeds were sown in the field in 2014. Samples were taken from the young leaves of the plants and preserved at -80oC in same year. RAPD and ISSR primers were used in the study. The bands obtained as a result of PCR were recorded and the data of both methods were also evaluated by combining them.
Result: In the study, 5 RAPD and 4 ISSR primers were used and a total of 49 bands were obtained. Of 29 bands obtained using RAPD primers, 20 were found to be polymorphic and of 20 bands obtained using ISSR primers, 15 were found to be polymorphic. It was found that there was a very low correlation between the two methods. Using RAPD and ISSR markers and RAPD + ISSR combination, the similarity index among populations was found to be between 0.25-0.95, 0.5-1.00 and 0.45-0.91, respectively. The Nei’s genetic diversity index was found to be between 0.3365, 0.2656 and 0.3018 with RAPD, ISSR primers and RAPD + ISSR combination, respectively. Based on the dendrograms obtained using RAPD, ISSR primers and RAPD + ISSR combination, the populations under analysis were classified into 3, 3 and 5 groups, respectively. With this study, the closest populations were identified and a significantly high genetic diversity was detected.

  1. Avcı, S., Ilhan, E., Erayman, M., Sancak, C. (2014). Analysis of onobrychis genetic diversity using SSR markers from related legume species. The Journal of Animal and Plant Sciences. 24(2): 556-566.

  2. Demdoum, S., Munoz, F., Delgado, I., Valderrabano, J., Wunsch, A. (2012). EST-SSR cross-amplification and genetic similarity in Onobrychis genus. Genet Resour Crop Evol. 59: 253-260

  3. Dehghan-Shoar, M., Hampton, J.G., Gardiner, S.E. (1997). Genetic analysis among and within populations forming ecotypes and cultivar of alfalfa, Medicago sativa (Leguminosae), using RAPD fragments. Plant Systematics and Evolution, 208: 107-119.

  4. Delgado, I., Salvia, J., Buil, I. andrés, C. (2008). The agronomic variability of a collection of sainfoin accessions. Spanish Journal of Agricultural Research. 6(3): 401-407

  5. Dos Santos, J. B., Nienhuis, J., Skroch, P., Tivang, J., Slocum, M. K. (1994). Comparison of RAPD and RFLP genetic markers in determining genetic similarity among (Brassica oleracea L.) genotypes. Theoretical and Applied Genetics. 87: 909-915.

  6. Doyle, J.J., Doyle, J.L. (1987). A rapid DNA isolation procedure from small quantities of fresh leaf tissues. Phytochem. Bull. 19: 11-15.

  7. Ertus, M.M., Sabancı, C.O., Sensoy, S. (2014). The Determination of Moleculer Diversity Among Some Alfalfa (Medicago sativa L.) Ecotypes Using RAPD Markers. Yyu. J. Agr. Scı. 24(1): 7-15. 

  8. Gherardi, M., Mangin, B., Goffinet, B., Bonnet, D., Huguet, T. (1998). A method to measure genetik distance between allogamous populations of alfalfa (Medicago sativa) using RAPD moleculer markers. Theor. Appl. Genetics. 96: 406-412.

  9. Hesamzadeh Hejazi, S.M., Ziaei Nasab, M. (2010). Cytotaxonomy of some Onobrychis (Fabaceae) species and populations in Iran. Caryologia. 63(1): 18-31.

  10. Hayot Carbonero, C., Mueller-Harvey, I., Brown, T.A., Smith, L. (2011). Sainfoin (Onobrychis viciifolia): A beneficial forage legume. Plant Genetic Resources: Characterization and Utilization. 9(1): 70-85.

  11. Hejrankesh, N., Mousavizadeh, S.A., Haghighi, A.R., Rashidi, V. (2014). Evaluation of genetic diversity of sainfoin (Onobrychis viciifoila scop.) Landraces using rapd markers. Journal of current research in science. 2(6): 739-748.

  12. Kempf, K., Mora-Ortiz, M., Smith, L.M.J., Kölliker, R., Skøt, L. (2016). Characterization of novel SSR markers in diverse sainfoin (Onobrychis viciifolia) germplasm. Genetics. 17: 124.

  13. Kumar, P., Gupta, V.K., Misra, A.K., Modi, D.R., Pandey, B.K. (2009). Potential of Molecular Markers in Plant Biotechnology. Plant Omics Journal. 2(4): 141-162.

  14. Labate, J.A. (2000). Software for population genetic analyses of molecular marker data. Crop. Sci. 40: 1521-1528.

  15. Mantel, N.A. (1967). The detection of disease clustering and a generalized regression approach. Cancer research. 27: 209-220. 

  16. Mengoni, A., Gori, A., Bazzicalupo, M. (2000). Use of RAPD and microsatellite (SSR) variation to ases genetic relationships among populations of tetraploid alfalfa, Medicago sativa. Plant Breeding. 119: 311-317.

  17. Mohajer, S., Jafari, A.A., Taha, R.M., and Ahmad, A.B.A. (2012). Evaluation of yield and morphological traits in 72 genotypes of sainfoin (Onobrychis viciifolia Scop) through factor analysis. Legume Research. 35(2): 132-137.

  18. Nei, M. (1973). Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA. 70: 3321-3323.

  19. Nosrati, H., Feizi, M.A.H., Tarrah, S.S., Haghighi, A.R. (2012). Population GeneticVariation In Sainfoin (Fabaceae) Revealed By Rapd Markers. Analele Universitãþii din Oradea–Fascicula Biologie Tom. XIX. 1: 11-16.

  20. Nosrati, H., Feizi, M.A.H., Latifian, F., Haghighi, A.R. (2016). Eco- Geographical variations of ISSRs among populations of Onobrychis viciifolia (Sainfoin, Fabacae). Analele Universitãþii din Oradea, Fascicula Biologie. 2: 62-66.

  21. Özkan, S., Bilgen, B.B. (2018). Genetic Characterization of Sainfoin (Onobrychis viciifolia) Varieties and Populations Using Microsatellite Markers. Journal of Tekirdag Agricultural Faculty. 16(1). 

  22. Paredes, M., Becerra, V., Rojo, C., Del Pozo, A., Ovalle, C., Aronson, J. (2002). Ecotypic differentiation in Medicago polymorpha L. along an environmental gradient in central Chile. RAPDs studies show little genetic divergence. Euphtica. 123: 431-439.

  23. Petolescu, C., Nedelea, G. (2009). Genetic Diversity Analysis of the In Vitro Regenerated Alfalfa Plants Using Inter Simple Sequence Repeat (ISSR) Markers. Romainan Biotechnological Letters. 14(6): 4882-4886.

  24. Rasouli, M., Jafari, A.A., Tabaei-Aghdaei, S.R., Salehi, P. (2013). Assessment of genetic variability of 36 populations of Sainfoin (Onobrychis sativa) based on RAPD markers. International Journal of Biosciences. 3(10): 15-26.

  25. Rohlf, F.J. (1997). NTSYS-Pc: Numerical Taxonomy and Multivariate Analysis System. Exeter Software, New York.

  26. Sanghani, J., Golakiya, B.A., Dhedhi, K.K. and Patel, S.V. (2015). Molecular characterization of mung bean (Vigna radiata L.) genotypes through RAPD, ISSR and SSR markers. Legume Research. 38: 452-456.

  27. Semagn, K., Bjørnstad, A., Ndjiondjop, M.N. (2006). An overview of molecular marker methods for plants. African Journal of Biotechnology. 5(25): 2540-2568.

  28. Sepet, H., Emre, Ý., Kiran, Y., Kurþat, M., Sahin, A. (2011). Karyological studies on eight species of Onobrychis genus in Turkey. Biologia. 66: 996-1002.

  29. Sharifi, S.M. (2016). Assessment Genetic Diversity Landraces In Onobrychis Sativa Using SSR, RAPD and SRAP Markers. Journal of Agriculture Biotechnology. 1(2): 91-95.

  30. Shen, S., Chai,X., Zhou, Q., Luo, D., Wang, Y., Liu, Z. (2019). Development of polymorphic EST-SSR markers and characterization of the autotetraploid genome of sainfoin (Onobrychis viciifolia). Peer J. 7:e6542. http://doi.org/ 10.7717/peerj.6542.

  31. Touil, L., Guesmi, F., Fares, K., Ferchichi, A. (2008). Genetic diversity of some Mediterranean populations of the cultivated alfalfa (Medicago sativa L.) using ISSR markers. Biotechnology. 7(4): 808-812.

  32. Tucak, M., Popovic, S., Cupic, T., Grljusic, S., Bolaric, S., Kozumplik, V. (2008). Genetic diversity of alfalfa (Medicago spp.) estimated by molecular markers and morphological characters. Periodicum Biologorum. 110(3): 243-249.

  33. Yeh, F.C., Yang, R.C., Boyle, T.B.J., Ye, Z.H., Mao, J.K. (1997). POPGENE, the User Friendly Shareware for Population Genetic Analysis. University of Alberta, Canada. Molecular Biology and Biotechnology Centre.

  34. Yildiz, M., Ekbic, E., Keles, D., Sensoy, S., Abak, K. (2011). Use of ISSR, SRAP and RAPD markers to assess genetic diversity in Turkish melons, Scientia Horticulturae. 130: 349-353.

  35. Zarrabian, M., Majidi, M.M. (2015). Genetic diversity and relationships within and among Onobrychis species using molecular markers. Turk J Bot. 39: 681-692. 

Editorial Board

View all (0)