Legume Research

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Research Article

Legume Research, volume 44 issue 10 (october 2021) : 1164-1171

​Association of ISSR and ISSR-RGA Markers with Powdery Mildew Resistance Gene in Mungbean

P.A. Tantasawat, O. Poolsawat, C. Kativat, K. Arsakit, P. Papan, W. Chueakhunthod, P. Pookhamsak
1School of Crop Production Technology, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang District, Nakhon Ratchasima 30000, Thailand.

  • Submitted28-12-2020|

  • Accepted23-06-2021|

  • First Online 05-08-2021|

  • doi 10.18805/LR-606

Cite article:- Tantasawat P.A., Poolsawat O., Kativat C., Arsakit K., Papan P., Chueakhunthod W., Pookhamsak P. (2021). ​Association of ISSR and ISSR-RGA Markers with Powdery Mildew Resistance Gene in Mungbean. Legume Research. 44(10): 1164-1171. doi: 10.18805/LR-606.

ABSTRACT

Background: Powdery mildew (PM) possesses a wide range of host plants, including mungbean that causes severe yield reductions. Using resistant varieties is an economically and environmentally effective approach in controlling the disease.
Methods: In this study, the genetic basis of inheritance and marker loci for PM resistance were investigated using 126 F2:9 recombinant inbred lines (RILs) raised from resistant and susceptible parents, V4785 and CN72, respectively. PM reaction in these RILs was visually scored from natural infection in the field and the segregation pattern was determined by the chi-square test (χ2). 
Result: The results revealed the segregation ration of 1:1, indicative of a dominantly inherited resistance gene controlling resistance against PM in V4785. Observation of potential polymorphism in two parents and two different bulks, each containing 10 RIL individuals showing either the highest resistance or susceptibility, identified 2 and 37 putatively linked inter-simple sequence repeat (ISSR) and ISSR-anchored resistance gene analog (ISSR-RGA) markers, respectively. Of these, 1 ISSR and 3 ISSR-RGA markers were stably inherited and linked to PM resistance (P<0.01). These linked marker systems may prove useful for facilitating the improvement of a durable resistant variety through marker-assisted selection in mungbean breeding programs.

REFERENCES


  1. Ali, Z., Xu, Z.L., Zhang, D.Y., He, X.L., Bahadur, S. and Yi, J.X. (2011). Molecular diversity analysis of eggplant (Solanum melongena) genetic resources. Genetics and Molecular Research. 10(2): 1141-55.

  2. Bainade, P.S., Kale, A.A., Kumbhar, S.D. and Deshmukh, S.G. (2014). Inter simple sequence repeats (ISSR) based polymorphism for powdery mildew resistance in green gram [Vigna radiata (L.) Wilczek]. Journal of Cell and Tissue Research. 14(3): 4547-50.

  3. Barakat, M.N., Milad, S.I., El-Shafei, A.M. and Khatab, S.A. (2008). Genetic analysis and identification of RAPD markers linked to northern corn leaf blight disease resistance in a white maize population. Journal of King Abdulaziz University-Meteorology Environment and Arid Land Agriculture Sciences. 20(1): 45-61.

  4. Chen, X.M., Line, R.F. and Leung, H. (1998). Genome scanning for resistance-gene analogs in rice, barley and wheat by high-resolution electrophoresis. Theoretical and Applied Genetics. 97: 345-55.

  5. Dev, S.S., Poornima, P. and Venu, A. (2018). Molecular characterization and phylogenetic analysis of NBS-LRR genes in wild relatives of eggplant (Solanum melongina L.). Indian Journal of Agricultural Research. 52: 167-71.

  6. Feuillet, C., Schachermayr, G. and Keller, B. (1997). Molecular cloning of a new receptor-like kinase gene encoded at the Lr10 disease resistance locus of wheat. The Plant Journal. 11: 45-52. 

  7. Humphry, S.M.E., Magner, T., McIntyre, C.L., Aitken, E.A. and Liu, C.L. (2003). Identification of major locus conferring resistance to powdery mildew (Erysiphe polygoni D.C.) in mungbean (Vigna radiata L. Wiczek) by QTL analysis. Genome. 46, 738-44. 

  8. Kasettranan, W., Somta, P. and Srinives, P. (2010). Mapping of quantitative trait loci controlling powdery mildew resistance in mungbean [Vigna radiata (L.) Wilczek]. Journal of Crop Science and Biotechnology. 13(3): 155-61.

  9. Khajudparn, P. (2009). Characters associated with yield potential and development of molecular markers for powdery mildew resistance in mungbean. Ph.D. Thesis, Suranaree University of Technology, Thailand.

  10. Khajudparn, P., Wongkaew, S. and Tantasawat, P. (2010). Identification of genes for powdery mildew resistance in mungbean. Journal of Life Sciences. 4: 25-29.

  11. Kour, G., Bakshi, P., Wali, V.K. and Jastotia, A. (2011). Role of molecular markers in some perennial crops-brief review. Agricultural Reviews. 32(4): 256-67.

  12. Levesque, R. and SPSS Inc. (2006). SPSS Programming and Data Management, 3rd Edn. SPSS Institute, Somers, New York. 

  13. Lodhi, M.A., Ye, G., Weeden, N.F. and Reisch, B.I. (1994). A simple and efficient method for DNA extraction from grapevine cultivars and Vitis species. Plant Molecular Biology. 12: 6-13.

  14. Mahanil, S., Reisch, B.I., Owens, C.L., Thipyapong, P. and Laosuwan, P. (2007). Resistance gene analogs from Vitis cinerea, Vitis rupestris and Vitis hybrid Horizon. American Journal of Enology and Viticulture. 58: 484-93.

  15. Nair, R.M., Pandey, A.K., War, A.R., Rao, B.H., Shwe, T., Alam A., Pratap, A., Malik, S.R., Karimi, R., Mbeyagala, E.K., Douglas, C.A., Rane, J. and Schafleitner, R. (2019). Biotic and abiotic constraints in mungbean production-progress in genetic improvement. Frontiers in Plant Science. 10: 1340-63.

  16. Poolsawat, O., Kativat, C., Arsakit, K. and Tantasawat, P.A. (2017). Identification of quantitative trait loci associated with powdery mildew resistance in mungbean using ISSR and ISSR-RGA markers. Molecular Breeding. 37(150): 150-61. 

  17. Reddy, K.S., Pawar, S.E. and Bhatia, C.R. (1994). Inheritance of powdery mildew (Erysiphe polygoni DC) resistance in mungbean [Vigna radiata (L.) Wilczek]. Theoretical and Applied Genetics. 88: 945-48. 

  18. Tantasawat, P.A., Poolsawat, O., Arsakit, K. and Papan, P. (2020). Identification of ISSR, ISSR-RGA and SSR markers associated with Cercospora leaf spot resistance gene in mungbean. International Journal of Agriculture and Biology. 23: 447-53.

  19. Tirnaz, S., Zhang, Y. and Batley, J. (2020). Genome-wide mining of disease resistance gene analogs using conserved domains. Methods and Protocols. 2107: 365-75.

  20. Uma, M.S., Hegde, N. and Hittalmani, S. (2016). Identification of SSR marker associated with rust resistance in cowpea (Vigna unguiculata L.) using bulk segregant analysis. Legume Research. 39: 39-42.

  21. Wei, C., Chen, J. and Kuang, H. (2016). Dramatic number variation of R genes in Solanaceae species accounted for by a few R gene subfamilies. PLoS ONE. 11(2): e0148708.

  22. Yu, S.C., Lin, P.C. and Lin, J. (2011). Effects of extrusion processing condition on the physic-chemical properties of mungbean extrudates. In: Book of Proceedings of the 12th Asian food conference 2011, Bangkok. pp 524-526.
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