The Electrophoretic Contribution to the Solutions of Taxonomical Problems of Some Lathyrus L. Taxa Growing in Turkey

DOI: 10.18805/LR-569    | Article Id: LR-569 | Page : 401-406
Citation :- The Electrophoretic Contribution to the Solutions of Taxonomical Problems of Some Lathyrus L. Taxa Growing in Turkey.Legume Research-An International Journal.2021.(44):401-406
İ. Emre, H. Genc, A. Sahin hagetr@yahoo.com
Address : Department of Science Education, Faculty of Education, Burdur Mehmet Akif University, Burdur, 15100, Turkey. 
Submitted Date : 30-05-2020
Accepted Date : 10-08-2020

Abstract

Background: The seed proteins are used as molecular markers to clarify the systematic problems. Also, electrophoretic techniques are safe tools to identify the seed proteins. In present study, it was used the SDS-PAGE technique to solve the taxonomical problems of eight taxa of genus Lathyrus belong to three sections Orobus, Lathyrostylis and Pratensis according to the globulin B and glutelin. 
Methods: The seed materials were collected from natural habitats and 0.5 g seed were homogenized and centrifuged based on extraction procedure. In addition, the amounts of seed storage protein subfractions were determined by using protein assay. The electrophoretic analysis were performed according to the 12% SDS-PAGE. The gel documentation system (Bio-Rad, USA) was used to analyse the electrophoretic data and UPGAMA was used to construct the dendogram to show the relationships among the species under focus. 
Results: Current study showed that the species of section Pratensis were different from species of sections Orobus and Lathyrostylis. The differences among the studied taxa shown clearly and all studied taxa were choosen from the protein patterns. Furthermore, present study demonstrated that L. nivalis has the highest globulin B and glutelin. Results of the present study generally supported the morphological data. 

Keywords

Cluster analysis Electrophoresis Lathyrus Seed storage proteins

References

  1. Abou-El-Enain, M.M., Loufty, M.H.A., Shehata, A.A. (2007). Seed surface characters and their systematic significance in the genus Lathyrus (Leguminosae, Papilionoideae, Vicieae). Feddes Repertorium. 118(7-8): 269-285.
  2. Akter, L., Mahbub, M., Habib, M.A., Alam S.S. (2015). Characterization of three varieties of Lathyrus sativus L. by fluorescent karyotype and RAPD analysis. Cytologia. 80(4): 457-465.
  3. Allkin, R., Macfarlane, T.D., White, R.J., Bisby, F.A., Adey, M.E., (1983). Names and synonyms of species and subspecies in the Vicieae. Vicieae Database Project Publication 2: Southampton, UK.
  4. Arslan, M. (2017). Fatty acid characteristics of grass pea (Lathyrus sativus) in an East Mediterranean environment. Cogent Chemistry. 3(1): 1-9.
  5. Asmussen, C.B. and Liston, A. (1998). Chloroplast DNA characters, phylogeny and classification of Lathyrus (Fabaceae). American Journal of Botany. 85(3): 387-401.
  6. Badr, A., Shazly, H.N., El Rabey, H., Watson, L.E. (2002). Systematic relationships in Lathyrus sect. Lathyrus (Fabaceae) based on amplified fragment length polymorphism (AFLP) data. Canadian Journal of Botany. 80: 962-969.
  7. Bagci, E., Genç, H. and Sahin, A. (2001). Fatty Acid Composition of four Lathyrus aphaca L. varieties, A chemotaxonomic approach. Pak. J. Biol. Sci. 4(7): 872-874. 
  8. Bassler, M. (1966). Die stellung des subgenus Orobus (L.) Baker in der Gattung Lathyrus L. und seine systematiche Gliederung. Feddes Repertorium. 72: 69-97.
  9. Belaid, Y., Chtourou-Ghorbel, N., Marrakchi, M., Trifi-Farah, N. (2006). Genetic diversity within and between populations of Lathyrus genus (Fabaceae) revealed by ISSR markers. Genetic Resources and Crop Evolution. 53: 1413-1418. 
  10. Ben Brahim, N., Salhi, A., Chtourou, N., Combes, D., Marrakchi, M. (2002). Isozymic polymorphism and phylogeny of 10 Lathyrus species. Genetic Resources and Crop Evolution. 49: 427-436. 
  11. Chtourou-Ghorbel, N., Lauga, B., Combes, D. and Marrakchi, M. (2001). Comparative genetic diversity studies in the genus Lathyrus using RFLP and RAPD markers. Lathyrus Lathyrism Newsletter. 2: 62-68.
  12. Chtourou-Ghorbel, N., Lauga, B., Ben Brahim, N., Combes, D., Marrakchi, M. (2002). Genetic variation analysis in the genus Lathyrus using RAPD markers. Genetic Resources and Crop Evolution. 49: 363-370. 
  13. Cildir, H., Kahraman, A., Dogan, M., Buyukkartal, H.N. (2017). Comparative anatomical and micromorphological studies on some species of Lathyrus L. section Lathyrus (Papilionoideae, Fabaceae). Commagene Journal of Biology. 1(1): 42-50. 
  14. Czefranova, Z. (1971). Conspectus systematis generis Lathyrus L.    Novitates Systematicae Plantarum Vasculurum (Leningrand). 8: 191-201.
  15. Davis, P.H. (1970). Lathyrus L. Flora of Turkey and East Aegean Islands. 3. Edinburgh University Press, Edinburgh.
  16. Davis, P.H., Mill, R.R., Kit, T. (1988). Flora of Turkey and East Aegean Islands 10. Edinburgh University Press, Edinburgh.
  17. Dogan, M., Kence, A., Tigin, C. (1992). Numerical taxonomic study on Turkish Lathyrus (Leguminoseae). Edinburgh Journal of Botany. 49(3): 333-341.
  18. El-Shanshoury, A.R. (1997). The use of seed proteins revealed by SDS-PAGE in taxonomy and phylogeny of some Lathyrus species. Biologia Plantarum. 39(4): 553-559.
  19. Emre, I., Turgut-Balik, D., Genc, H., Sahin, A. (2006). The use of seed proteins revealed by SDS-PAGE in taxonomy of some Lathyrus L. species grown in Turkey. Pakistan Journal of Biological Sciences. 9(12): 2358-2361.
  20. Genc, H., Sahin, A. (2011). A new species of Lathyrus L. (Fabaceae) from Turkey. J. Syst. Evol. 49(5): 505-508. 
  21. Guner, F., Ozhatay, N. (2000). Lathyrus L. in Guner A, Ozhatay N, Ekim, T, Baser KHC. eds. Flora of Turkey and the East Aegean Islands 11. Edinburgh University Press Edinburgh.
  22. Gunes, F. (2012). Seed morphology and their systematic importance of Lathyrus taxa belonging to Platystylis (=Lathyrostylis) section (Fabaceae) from Turkey. African Journal of Agricultural Research. 7(2): 265-277.
  23. Kafadar, F.N., Ozkan, A., Can, C., Mart, D., Ceyhan, E. (2019). Genetic and biochemical properties of Cicer spp reveal distinction between wild and cultivated chickpea genotypes. Legume Research. 42(1): 1-9. 
  24. Kenicer, G.J., Kajita, T., Pennington, R.T., Murata, J. (2005). Systematics and biogeography of Lathyrus (Leguminosae) based on internal transcribed spacer and cpDNA sequence data. American Journal of Botany. 92(7): 101-110. 
  25. Kupicha, F.K. (1983). The infrageneric structure of Lathyrus. Notes RBG Edinburg, 41(29): 209-244.
  26. Ladizinsky, G., Hymowitz, T. (1979). Seed protein electrophoresis in taxonomic and evolutionary studies. Theor. Appiled Genet. 54: 145-151.
  27. Laemmli, U.K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227: 680-685.
  28. Marghali, S., Zitouna, N., Gharbi, M., Fadhlaoi, I., Trifi-Farah, N. (2014). Evolution of rbcL among Lathyrus and Kupicha’s classification. Genet. Mol. Res. 13(4): 8729-8739.
  29. Marghali, S., Touati, A., Gharbi, M., Sdouga, D., Trifi-Farah, N. (2016). Molecular phylogeny of Lathyrus species: insights from sequence-related amplified polymorphism markers. Genetics and Molecular Research. 15(1): 1-14.
  30. Nikolic, Z., Dordevic, V., Torbica, A., Mikic, A. (2012). Legumes seed storage proteins characterization by SDS-PAGE and Lab-on-a-Chipelectrophoresis. Journal of Food Consumption and Analysis. 28: 75-80. 
  31. Oskoueiyan, R., Osaloo, S.K., Amirahmadi, A. (2014). Phylogeny of the Genus Lathyrus (Fabaceae-Fabeae) Based on cpDNA matK Sequence in Iran. Iran J. Biotech. 12(2): 41-48. 
  32. Ozbek-Yazici, S., Ozmen, I., Yildirim, B., Genc, H., Ozeloglu, B., Gulsun, M., Elmas, H., Ozcaka, S. (2019). Biochemical composition of Lathyrus L. seeds: antioxidant activities, phenolic profiles, â-ODAP and protein contents. Legume Research. DOI: 10.18805/LR-516.
  33. Osborne, T.B. (1924). The Vegetable Proteins. Longmans-Green: London, UK.
  34. Przybylska, J., Z-Przybylska, Z., Krajewski, P. (2000). Diversity of seed globulins in Lathyrus sativus L. and some related species: Genetic Resources and Crop Evolution. 47: 239-246. 
  35. Radwan, S.A., El-Koly, A.S., Sammour, R.H. (2013). Genetic variation among accessions of Lathyrus inconspicuous (L.) as revealed by SDS Polyacrylamide Gel Electrophoresis. Acta Agriculturae Slovenica.101-1.
  36. Sammour, R.H., Fahmee, S., Mustafa, A.-E., Mustafa, Taher, W. (2019). Isozyme analysis of genetic variability and population structure of Lathyrus sativus L. germplasm. Legume Research. 42(6): 750-756.
  37. Sinha, K.N., Meenakshi, S., Kumar C. (2012). Electrophoretic Study of Seed Storage Protein in Five Species of Bauhinia. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS). 4(2): 8-11.
  38. Sofalian, O., Bandarian, P., Asghari, A., Sedghi, M., Firoozi, B. (2015). Identification of seed storage protein polymorphism in some soybean (Glycine max Merril) genotypes using SDS-PAGE technique. Yyu. J. Agr. Sci. 25(2): 127-133.
  39. Vaz, A.C., Pinheiro, C., Martins, J.M.N., Ricardo, C.P.P. (2004). Cultivar discrimination of Portuguese Lupinus albus by seed protein electrophoresis: the importance of considering “glutelins” and glycoproteins. Field Crops Research. 87: 23-34.

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