Legume Research

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Research Article

Legume Research, volume 44 issue 5 (may 2021) : 501-507

Cytogenetic Diversity and Characterization of Vicia sativa Subspecies

Berk Benlioglu
1Department of Field Crops, Agriculture Faculty, Ankara University, 06110, Ankara, Turkey.

  • Submitted08-05-2020|

  • Accepted22-10-2020|

  • First Online 29-12-2020|

  • doi 10.18805/LR-567

Cite article:- Benlioglu Berk (2020). Cytogenetic Diversity and Characterization of Vicia sativa Subspecies. Legume Research. 44(5): 501-507. doi: 10.18805/LR-567.

ABSTRACT

Background: Vicia sativa L. is variable genus comprised of several subspecies. Close relative species and subspecies of the cultivated species are easily usable gene sources because they have gained resistance against a wide range of biotic and abiotic stresses. The main objectives of this study are to identify and describe the cytogenetical and karyological characteristics of subspecies in the Vicia sativa complex.
Methods: The research material consisted of multiple entries collected from the five subspecies of nine taxa. All cytological observations made from root tips. Six chromosomal parameters (chromosome length, relative length, long arm length, short arm length, arm ratio and centromeric index) and five karyotype asymmetric parameters (difference in relative length, total form percentage, intrachromosomal asymmetry index, interchromosomal asymmetry index and mean centromeric asymmetry) were determined.
Result: It was determined that the chromosome number of subspecies were 2n =10-12. The haploid chromosome lengths of subspecies were 15.86-33.88 µm and the average chromosome lengths varied between 2.64-5.65 µm. According to the intrachromosomal karyotype asymmetry index analysis, subsp. segetalis was the most asymmetric karyotype and subsp. sativa “Antalya” was the most symmetric karyotype. According to the interchromosomal karyotype asymmetry index analysis, subsp. angustifolia was the most asymmetric karyotype and subsp. nigra was the most symmetric karyotype.

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