Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000)

DOI: 10.18805/LR-554    | Article Id: LR-554 | Page : 175-184
Citation :- Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000).Legume Research-An International Journal.2021.(44):175-184
Mohamed Milad Draweel, Andy Soegianto, Lita Soetopo, Kuswanto Kuswanto M_daraweil@yahoo.com
Address : Department of Agronomy, Faculty Agriculture, Brawijaya University, Malang, Indonesia.
Submitted Date : 6-03-2020
Accepted Date : 18-06-2020

Abstract

Background: Bambara groundnut has the ability to drought tolerance and has high nutritional value and is one than Legume crops untapped, which need attention. Therefore in this research, it will look at the relationship between osmotic stress caused by a concentration of PEG and drought tolerance to get some genotypes has ability drought tolerance. 
Methods: The experiment was conducted in the Tissue culture Laboratory, Department of Agronomy, Faculty of Agriculture, the University of Brawijaya, Malang, Indonesia in January-March 2019. The objective of the experiment was an evaluation of some morphological criteria to drought tolerance on the seedling for thirty genotypes of Bambara groundnut using polyethylene glycol (PEG) 6000 by three concentrations (0%, 5% and 10%). A complete randomized design was used for this experiment with three replicates. 
Result: The 10% PEG was the most suitable concentration to select Bambara groundnut lines for drought tolerance. Genotypes give different responses to concentrations of PEG. In susceptible lines, there are germination constraints, such as Prevent and germination delayed, the formation of hypocotyl length, root length, fresh weight and dry matter, while drought-tolerant lines can germinate normally. Obtained six lines that were tolerant to drought, five local, namely BBL 1.1, PWBG 5.2.1, PWBG 6, SS 2.4.2, SS 3.4.2 and one from Thailand which is Tvsu 86.

Keywords

Bambara groundnut Drought tolerance Polyethylene glycol Selected lines

References

  1. Abobatta, W. (2016). nutritious seeds for a sustainable future, international year of Pulses, at Horticulture Research Institute. Agriculture Research Center, 2016. 
  2. Achard, P., Gusti, A., Cheminant, S., Alioua, M., Dhondt, S., et al. (2009). Gibberellin signaling controls cell proliferation rate in Arabidopsis. Current Biology. 19(14): 1188-1193. 
  3. Atta, S., Maltese, S. and Cousin, R. (2004). Protein content and dry weight of seeds from various pea genotypes. 24(5): 257-266.
  4. Bibi, A., Sadaqat, H. A., Akram, H. M. and Mohammed, M. I. (2010). Physiological markers for screening sorghum (Sorghum bicolor) germplasm under water stress condition. Int. J. Agric. Biol. 12(3): 451-455. 
  5. Bolat, I., Dikilitas, M., Ercisli, S., Ikinci, A. and Tonkaz, T. (2014). The effect of water stress on some morphological, physiological and biochemical characteristics and bud success on apple and quince rootstocks. The Scientific World Journal. 2014. 
  6. Chandra, K., Nandini, R., Gobu, R., Kumar, C.B. and Muthuraju, R. (2019). Insight into floral biology and ancillary character- -istics of underutilized legume-Bambara groundnut [Vigna subterranea (L.) Verdc.]. Legume Research-An International Journal. 42(1): 96-101. 
  7. Dhanda, S., Sethi, G. and Behl, R. (2004). Indices of drought tolerance in wheat genotypes at early stages of plant growth. Journal of Agronomy and Crop Science. 190(1): 6-12. 
  8. EL SHIMI, N.M., Luh, B. and SHEHATA, A.E.T. (1980). Changes in microstructure, starch granules and sugars of germinating broad beans. Journal of Food Science. 45(6): 1652-1657. 
  9. Evaneri, N. (1957). Les problèmes physiologiques critères de laermination Bull. Soc, Physiol.Vege. 3: 105-124. 
  10. Hasan, M., Uddin, K., Mohamed, M.T.M. and Zuan, A.T.K. (2018). Nitrogen and phosphorus management for Bambara groundnut (Vigna subterranea) production-A review. Legume Research-An International Journal. 41(4): 483-489. 
  11. Hassen, A., Rethman, N., Apostolides, Z. and Van Niekerk, W. (2007). Influence of moisture stress on growth, dry matter yield and allocation, water use and water-use efficiency of four Indigofera species. African Journal of Range and Forage Science. 24(1): 25-34. 
  12. Karjalainen, R. and Kortet, S. (1987). Environmental and genetic variation in protein content of peas under northern growing conditions and breeding implications. Agricultural and Food Science. 59(1): 1-9. 
  13. Kaydan, D. and Yagmur, M. (2008). Germination, seedling growth and relative water content of shoot in different seed sizes of triticale under osmotic stress of water and NaCl. African Journal of Biotechnology. 7(16): 2862-2868. 
  14. Koné, M., Koné, T., Silué, N., Soumahoro, A.B. and Kouakou, T.H. (2015). In vitro seeds germination and seedling growth of Bambara groundnut [Vigna subterranea (L.) Verdc. (Fabaceae)]. The Scientific World Journal. 2015. 
  15. Lhuillier-Soundélé, A., Munier-Jolain, N. G. and Ney, B. (1999). Dependence of seed nitrogen concentration on plant nitrogen availability during the seed filling in pea. European Journal of Agronomy. 11(2): 157-166. 
  16. Litvin, A.G., van Iersel, M.W. and Malladi, A. (2016). Drought stress reduces stem elongation and alters gibberellin-related gene expression during vegetative growth of tomato. Journal of the American Society for Horticultural Science. 141(6): 591-597. 
  17. Michel, B.E. and Kaufmann, M.R. (1973). The osmotic potential of polyethylene glycol 6000. Plant Physiology. 51(5): 914-916. 
  18. Mohammed, M., Shimelis, H. and Laing, M. (2020). Preliminary morphological characterization and evaluation of selected Bambara groundnut [Vigna subterranea (L.) Verdc.] genotypes for yield and yield related traits. Legume Research-An International Journal. 43(2): 157-164. 
  19. Ncube, B., Twomlow, S.J., Van Wijk, M.T., Dimes, J.P. and Giller, K.E. (2007). Productivity and residual benefits of grain legumes to sorghum under semi-arid conditions in south western Zimbabwe. Plant and Soil. 299(1-2): 1-15. 
  20. Pire, R., Pereira, A., Díez, J. and Fereres, E. (2007). Evaluación de la tolerancia a la sequía de un portainjerto venezolano de vid y posibles mecanismos condicionantes. Agrociencia. 41(4): 435-446. 
  21. Pond, W.G., Church, D.B., Pond, K.R. and Schoknecht, P. A. (2004). Basic Animal Nutrition and Feeding: John Wiley and Sons. ISBN: 978-0-471-21539-4. pp 608.
  22. Saio, K., Arai, K. and Watanabe, T. (1973). Fine structure of soybean seed coat and its changes on cooking. Cereal Science Today. 18: 197-201.
  23. Stanton, W., Doughty, J., Orraca-Tetteh, R. and Steele, W. (1966). Voandzeia subterranea Thouars. Grain Legumes in Africa. 128-133. 
  24. Swanevelder, C. (1998). Bambara groundnut (Vigna subterranea L. Verdc)-food for Africa. Grain Crops Institute, Potchefstroom, SA. 
  25. Swanson, B.G., Hughes, J.S. and Rasmussen, H.P. (1985). Seed microstructure: review of water imbibition in legumes. Food Structure. 4(1): 14. 
  26. Temegne, N., Foh, T., Taffouo, V., Wakem, G.A. and Youmbi, E. (2018). Effect of mycorrhization and soluble phosphate on growth and phosphorus supply of Voandzou [Vigna subterranea (L.) Verdc.]. Legume Research-An International Journal. 41(6): 879-884. 
  27. Vettakkorumakankav, N.N., Falk, D., Saxena, P. and Fletcher, R.A. (1999). A crucial role for gibberellins in stress protection of plants. Plant and Cell Physiology. 40(5): 542-548. 

Global Footprints