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Legume Research

Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000)

DOI: 10.18805/LR-554    | Article Id: LR-554 | Page : 175-184
Citation :- Evaluation of Some Morphological Criteria to Drought Tolerance on Seedling of Bambara Groundnut [Vigna subterranea (L.) Verdc.] using Polyethylene Glycol (PEG6000).Legume Research.2021.(44):175-184
Mohamed Milad Draweel, Andy Soegianto, Lita Soetopo, Kuswanto Kuswanto M_daraweil@yahoo.com
Address : Department of Agronomy, Faculty Agriculture, Brawijaya University, Malang, Indonesia.
Submitted Date : 6-03-2020
Accepted Date : 18-06-2020
First Online:

Abstract

Background: Bambara groundnut has the ability to drought tolerance and has high nutritional value and is one than Legume crops untapped, which need attention. Therefore in this research, it will look at the relationship between osmotic stress caused by a concentration of PEG and drought tolerance to get some genotypes has ability drought tolerance. 
Methods: The experiment was conducted in the Tissue culture Laboratory, Department of Agronomy, Faculty of Agriculture, the University of Brawijaya, Malang, Indonesia in January-March 2019. The objective of the experiment was an evaluation of some morphological criteria to drought tolerance on the seedling for thirty genotypes of Bambara groundnut using polyethylene glycol (PEG) 6000 by three concentrations (0%, 5% and 10%). A complete randomized design was used for this experiment with three replicates. 
Result: The 10% PEG was the most suitable concentration to select Bambara groundnut lines for drought tolerance. Genotypes give different responses to concentrations of PEG. In susceptible lines, there are germination constraints, such as Prevent and germination delayed, the formation of hypocotyl length, root length, fresh weight and dry matter, while drought-tolerant lines can germinate normally. Obtained six lines that were tolerant to drought, five local, namely BBL 1.1, PWBG 5.2.1, PWBG 6, SS 2.4.2, SS 3.4.2 and one from Thailand which is Tvsu 86.

Keywords

Bambara groundnut Drought tolerance Polyethylene glycol Selected lines

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