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Legume Research

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Research Article

Legume Research, volume 43 issue 4 (august 2020) : 573-579

Effects of Rhizobium Strain on the Growth, Nodulation, N2 Fixation and Ions Accumulation in Vicia Faba Plant Under Salt Stress

Boulbaba L’taief, Neila Abdi, Sihem Smari, Amel Ayari-Akkari, Mouna Jeridi, Manar D. Alsenidi, Bouaziz Sifi
1Biology Department, College of Sciences in Abha, King Khaled University, P.O. Box 960, Abha, Saudi Arabia.

  • Submitted27-03-2019|

  • Accepted21-08-2019|

  • First Online 04-10-2019|

  • doi 10.18805/LR-486

Cite article:- L’taief Boulbaba, Abdi Neila, Smari Sihem, Ayari-Akkari Amel, Jeridi Mouna, Alsenidi D. Manar, Sifi Bouaziz (2019). Effects of Rhizobium Strain on the Growth, Nodulation, N2 Fixation and Ions Accumulation in Vicia Faba Plant Under Salt Stress. Legume Research. 43(4): 573-579. doi: 10.18805/LR-486.

ABSTRACT

Vicia faba L.-rhizobia symbiosis is utilized in different biological ways to improve the productivity of faba beans. This research aims to analyze the effects of the Rhizobium strain on nodulation, N2 fixation, growth, and ion accumulation under salt stress in Viciafaba. The commercial cultivar of faba beans (Viciafaba L. var. minor) was inoculated with the Rhizobium leguminosarumbiovar, by considering viciae strains S10 and S16, after 15 days of growth. This inoculation was carried out in the solution culture consisting of two salt concentrations; 0 mmole l-1NaCl and 50 mmole l-1NaCl. The results revealed that under saline and non-saline conditions, S10 and S16 strains of Rhizobium leguminosarum resulted in the formation of ineffective and effective symbiosis with faba beans. However, the presence of salt stress resulted in increasing the biomass of nodule and nitrogen content. The concentrations of sodium and chloride, in shoot and root, were increased in the presence of salinity. However, potassium concentration was only increased in the shoot. With and without salinity, phosphorus concentration in the shoots was not modified. The results revealed that the salt tolerance of faba beans, inoculated with two strains of Rhizobium were found to possess association with their stable growth. Moreover, the salt tolerance of faba beans inoculated with two salts tolerant rhizobia was also associated with an increment in the capacity of faba beans to increase nodulation and the concentration of shoot N2, Na and Cl-content. In addition, salt tolerance of this variety, inoculated with Rhizobium strains was associated with a decrement in the concentration of K+ in shoot under the salt constraints.

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