Legume Research

  • Chief EditorJ. S. Sandhu

  • Print ISSN 0250-5371

  • Online ISSN 0976-0571

  • NAAS Rating 6.59

  • SJR .349 (2022) Q2

  • Impact Factor .669 (2022)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
BIOSIS Preview, ISI Citation Index, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India

D. Vijay Kumar Naik, B.V. Bhaskara Reddy, R. Sarada Jayalakshmi Devi, L. Prasanthi, R. Lakshminarayana Vemireddy, Akkari Srividhya, B.H. Chaithanya, T.M. Hemalatha, K. Sailaja
  • Email
1Department of Plant Pathology, Regional Agricultural Research Station, Acharya N.G. Ranga Agricultural University, Tirupati-517 502, Andhra Pradesh, India.
  • Submitted27-09-2021|

  • Accepted18-01-2022|

  • First Online 28-03-2022|

  • doi 10.18805/LR-4800

Cite article:- Naik Kumar Vijay D., Reddy Bhaskara B.V., Devi Jayalakshmi Sarada R., Prasanthi L., Vemireddy Lakshminarayana R., Srividhya Akkari, Chaithanya B.H., Hemalatha T.M., Sailaja K. (2022). Genetic Variability Studies of Yellow Mosaic Virus Infecting Blackgram [Vigna mungo (L.) Hepper] from Andhra Pradesh, India. Legume Research. 45(7): 907-913. doi: 10.18805/LR-4800.
Background: The productivity of blackgram is affected by many biotic and abiotic stresses. Among the biotic stresses, yellow mosaic disease (YMD) cause severe yield loss and it is caused by four distinct viruses (belongs to genus begomovirus) collectively known as yellow mosaic virus (YMV). Hence there is need to characterize various YMV isolates associated with blackgram in Andhra Pradesh. 
Methods: YMV infected blackgram samples were collected from East Godavari, Kurnool and Prakasam districts of Andhra Pradesh. The Rolling Circle Amplification (RCA) based full length MYMIV DNA-A and MYMV DNA-B of three isolates were cloned and sequenced.  
Result: Nucleotide sequence of full length DNA-A of MYMIV-East Godavari isolate showed >96% similarity at nucleotide and >90% at amino acid level with other MYMIV isolates in NCBI database. The complete DNA-A nucleotide sequence of MYMIV-Kurnool and MYMIV-Prakasam isolates shared >99% similarity at nucleotide and >98% at amino acid level with other MYMIV isolates. The complete nucleotide sequence of MYMV DNA-B of three isolates (East Godavari, Kurnool and Prakasam) had >97% homology with other MYMV DNA-B isolates from database. The predicted amino acid sequence of MYMV DNA-B of three isolates shared >96% homology with other MYMV-B isolates. The common region (CR) sequence similarity between MYMIV-As and MYMV-Bs of East Godavari and Kurnool isolates was 76.4% and 78.3% with Prakasam isolate. The divergence between the MYMIV-A and MYMV-B of present three isolates (East Godavari, Kurnool and Prakasam) under study were ranged from 22.2 to 22.6%.

  1. Archana, S., Venkatesh., Padmaja, A.S., Manjunatha, N and Nagaraju, N. (2017). Molecular detection and characterization of yellow mosaic virus (YMV) infecting blackgram (Vigna mungo L.) in Karnataka with the aid of coat protein (cp) gene. Journal of Food Legumes. 30(4): 277-280.

  2. Arguelo-Astorga, G.R., Guevara-Gonzalez, R.G., Herrera-Estrella, L.R., Rivera-Bustamante, R.F. (1994). Geminivirus replication origins have a group specific organization of iterative elements: A model for replication. Virology. 203: 90-100.

  3. Fauquet, C.M., Briddon, R.W., Brown, J.K., Moriones, E., Stanley, J., Zerbini, M and Zhou, X. (2008). Geminivirus strain demarcation and nomenclature. Archives of Virology. 153: 783-821.

  4. Haq, Q.M.I., Rouhibakhsh, A., Ali, A. and Malathi, V.G. (2011). Infectivity analysis of a blackgram isolate of Mungbean yellow mosaic virus and genetic assortment with MYMIV in selective hosts. Virus Genes. 2011. 42: 429-439.

  5. John, P., Sivalingam, P.N., Haq, Q.M.I., Kumar, N., Mishra, A., Briddon, R.W. and Malathi, V.G. (2008). Cowpea golden mosaic disease in Gujarat is caused by a mungbean yellow mosaic India virus isolate with a DNA B variant. Archives of Virology. 153: 1359-1365.

  6. Karthikeyan, A.S., Vanitharani, R., Balaji, V., Anuradha, S., Thillaichidambaram, P., Shivaprasad, P.V., Parameswari, C., Balamani, V., Saminathan, M. and Veluthambi, K. (2004). Analysis of an isolate of mungbean yellow mosaic virus (MYMV) with a highly variable DNA-B component. Archives of Virology.149: 1643-1652.

  7. Kumar, S., Bhaben, T., Sunil, K.M., Lingaraj, S. (2017). Molecular characterization and infectivity of mungbean yellow mosaic India virus associated with yellow mosaic disease of cowpea and mungbean. Biocatalysis and Agricultural Biotechnology. 11: 183-191.

  8. Lizardi, P.M., Hauang, X., Zhu, Z., Bray-ward, P., Thomas, D.C. and Ward, D.C. (1998). Mutation detection and single- molecule counting using isothermal rolling-circle amplification. Nature Genetics. 19(3): 225-432.

  9. Mishra, G.P., Dikshit, H.K., Ramesh, S.V., Tripathi, K., Kumar, R.R., Aski, M., Singh, A., Roy, A., Priti., Kumari, N., Dasgupta, U., Kumar, A., Praveen, S. and Nair, R.M. (2020). Yellow Mosaic Disease (YMD) of mungbean [Vigna radiata (L.) Wilczek]: Current status and management opportunities. Frontiers in Plant Science. 11: 1-24.

  10. Murray, M.G. and Thompson W.F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research. 8: 4321-4326.

  11. Nariani, T.K. (1960). Yellow mosaic of mung (Phaseolus aureus L.). Indian Phytopathology. 13: 24 29.

  12. Pant, V., Gupta, D., Roy Choudhury, N., Malathi, V.G., Varma, A. and Mukherjee, S.K. (2001). Molecular characterization of the rep protein of the blackgram isolate of Indian Mungbean yellow mosaic virus. Journal of General Virology. 82: 2559-2567.

  13. Prema, G.U. and Rangaswamy, K.T. (2018). Molecular characterization of coat protein gene of blackgram yellow mosaic virus (BGYMV) from Karnataka, India. International Journal of Current Microbiology and Applied Sciences. 7(7): 2225-2235.

  14. Reddy, B.V.B., Obaiah, S., Prasanthi, L., Sivaprasad, Y., Sujitha, A. and Krishna, T.G. (2015). Mungbean yellow mosaic India virus is associated with yellow mosaic disease of blackgram (Vigna mungo L.) in Andhra Pradesh, India. Archives of Phytopathology and Plant Protection. 48(4): 345-353.

  15. Satya, V.K., Alice, D., Malathi, V.G and Velazhahan, R. (2015). Molecular identification and genetic diversity of yellow mosaic virus in blackgram. Biochemical and Cellular Archives. 15(2): 475-479.

  16. Tamura, K., Dudley, J., Nei, M and Kumar, S. (2007). MEGA 7: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Molecular Biology and Evolution. 24: 1596-1599.

  17. Williams, F.J., Grewal, J.S. and Amin, K.S. (1968). Serious and new diseases of pulse crops in India in 1966. Plant Disease Reporter. 52: 300-304.

Editorial Board

View all (0)