Assessment of Genetic Diversity and Population Structure in Pea (Pisum sativum L.) Germplasm based on Morphological Traits and SSR Markers

DOI: 10.18805/LR-4751    | Article Id: LR-4751 | Page : 683-688
Citation :- Assessment of Genetic Diversity and Population Structure in Pea (Pisum sativum L.) Germplasm based on Morphological Traits and SSR Markers.Legume Research.2022.(45):683-688
Shalini Singh, B. Singh, V.R. Sharma, M. Kumar, U. Sirohi drshalinisinghhorti@gmail.com
Address : Department of Horticulture, Dr. Khem Singh Gill Akal College of Agriculture, Eternal University, Baru Sahib-173 001, Himachal Pradesh, India.
Submitted Date : 26-07-2021
Accepted Date : 19-10-2021


Background: The study was undertaken to assess the genetic diversity and genetic structure among fifty-five pea accessions using morphological traits and SSR markers.
Methods: A total of 55 pea accessions were analyzed using eleven phenotypic traits and twenty SSR markers. The data obtained by morphological and molecular profiling was used for the analysis of genetic diversity and for the estimation of genetic diversity estimates, correlation, principal components analysis and population structure.
Result: This study reveals that majority of genetic variation was due to variation within population and were clustered into two distinct groups, which reveals a high admixture within individuals. Accessions viz., VRP-82, VRP-320, VRP-194, VRP-375, EC-97280 and EC-8724, showed great diversity as compared to the other accessions based on both morphological and molecular markers. These accessions may assist in developing and planning breeding strategies aimed to produce new varieties in the future. 


Genetic diversity Pea Population structure SSR markers


  1. Bostein, D., White, R.L., Skolnick, M. and Davis, R.W. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisms. American Journal of Human Genetics. 32: 314-331. 
  2. Choudhury, P.R., Tanveer, H. and Dixit, G.P. (2007). Identification and detection of genetic relatedness among important varieties of pea (Pisum sativum L.) grown in India. Genetica. 130: 183-187. 
  3. Cupic, T., Tucak, M., Popovic, S., Bolaric, S., Grljusic, S. and Kozumplik, V. (2009). Genetic diversity of pea (Pisum sativum L.) accessions assessed by pedigree, morphological and molecular data. Journal of Food, Agriculture and Environment. 7: 343-348. 
  4. Doyle, J.J. and Doyle, J.L. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19: 11-15. 
  5. Excoffier, L., Smouse, P.E. and Quattro, J.M. (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: Application to human mitochondrial DNA restriction sites. Genetics. 131: 479-491. 
  6. Excoffier, L. (1993). Analysis of Molecular Variance (AMOVA) Version 1.55. In: Genetics and Biometry Laboratory, University of Geneva, Switzerland. 
  7. Handerson, C., Noren, S.K., Wricha, T., Meetei, N.T., Khanna, V.K., Pattanayak, A., Shiv, D., Choudhury, P.R. and Kumar, M. (2014). Assessment of genetic diversity in pea (Pisum sativum L.) using morphological and molecular markers. Indian Journal of Genetics. 74: 205-212. 
  8. Huang, Y., Liu, X., Cao, D., Chen, G., Li, S., Wang, G., Wang, J., Xu, S. (2021). Cross-species Amplification of Common Bean (Phaseolus vulgaris) EST-SSRs within Hyacinth Bean, Pea and Soybean. Legume Research. 44(3): 287- 291. 
  9. Jain, S., Kumar, A. and Mamidi, S. and McPhee, K. (2014). Genetic diversity and population structure among pea (Pisum sativum L.) cultivars as revealed by simple sequence repeat and novel genic markers. Molecular Biotechnology. 56: 925-938. 
  10. Kumar, M., Rakesh Sharma, V., Kumar, V., Sirohi, U., Chaudhary, V., Sharma, S., Saripalli, G., Naresh, R.K., Yadav, H.K., Sharma, S. (2019). Genetic diversity and population structure analysis of Indian garlic (Allium sativum L.) collection using SSR markers. Physiology Molecular Biology Plants. 25: 377-386. DOI: 10.1007/s12298-018-0628-y.
  11. Loridon, K., McPhee, K., Morin, J., Dubreuil, P., Pilet-Nayel, M.L., Aubert, G., Rameau, C., Baranger, A., Coyne, C., Lejeune- Henaut, I. and Burstin, J. (2005). Microsatellite marker polymorphism and mapping in pea (Pisum sativum L.). Theoretical and Applied Genetics. 111: 1022-1031. 
  12. Nagaraju, J., Reddy, K.D., Nagaraja, G.M. and Sethurama, B.N. (2001). Comparison of multilocus RFLPs and PCR-based marker systems for genetic analysis of the silkworm, Bombyx mori. Heredity. 86: 588-597.
  13. National Horticulture Board. (2019). Indian Horticulture Database. http://www.nhb.gov.in.
  14. Negisho, K., Teshome, A. and Keneni, G. (2017). Genetic diversity in Ethiopian field pea (Pisum sativum L.) germplasm collections as revealed by SSR markers. Ethiopian Journal of Agricultural Sciences. 27: 33-47.
  15. Peakall, R. and Smouse, P. (2006). GENEALE × 6: Genetic Analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes. 6: 288-295. 
  16. Prakash, N., Kumar, R., Choudhary, V.K. and Singh, C.M. (2016). Molecular assessment of genetic divergence in pea genotypes using microsatellite markers. Legume Research. 39: 183-188. 
  17. Prevost, A. and Wilkinson, M.J. (1999). A new system of comparing PCR primers applied to ISSR fingerprinting of potato cultivars. Theoretical and Applied Genetics. 98: 107-112.  
  18. Pritchard, J.K., Stephens, M. and Donnelly, P. (2000). Inference of population structure using multilocus genotype data. Genetics. 155: 945-959.
  19. Rahman, M., Hasan, M., Chowdhury, R.N., Ivy, N.A. and Hossain, M.M. (2013). Genetic diversity in vegetable pea (Pisum sativum L.). Bangladesh Journal of Plant Breeding and Genetics. 26: 35-40. 
  20. Rana, J.C., Rana, M., Sharma, V., Nag, A., Chahota, R.K. and Sharma, T.R. (2017). Genetic diversity and structure of pea (Pisum sativum L.) germplasm based on morphological and SSR markers. Plant Molecular Biology. 35: 118-129. 
  21. Rohlf, F.J. (1998). NTSYSpc Numerical Taxonomy and Multivariate Analysis System Version 2.0 User Guide. Applied Biostatistics Inc Setauket New York.
  22. Santalla, M., Amurrio, J.M. and De, Ron, A.M. (2001). Food and feed potential breeding value of green, dry and vegetable pea germplasm. Canadian Journal of Plant Sciences. 8: 601-610.
  23. Singh, S., Sharma, V.R., Nannuru, V.K.R., Singh, B. and Kumar, M.  (2021). Phenotypic Diversity of Pea Genotypes (Pisum sativum L.) Based on Multivariate Analysis. Legume Research. 44: 875-881. 
  24. Shrivastava, V., Lal, G.M., Chandra, K. and Singh, C.M. (2012). Estimation of genetic distance in field pea (Pisum sativum L.). Environment and Ecology. 30: 136-138.
  25. Smykal, P., Hybl, M., Corander, J., Jarkovsky, J., Flavell, A.J. and Griga, M. (2008). Genetic diversity and population structure of pea (Pisum sativum L.) varieties derived from combined retrotransposon, microsatellite and morphological marker analysis. Theoretical Applied Genetics. 117: 413-424. 
  26. Suman, S., Rani, B., Sharma, V.K., Kumar, H., Shahi, V.K. (2019). SSR marker based profiling and diversity analysis of mungbean [Vigna radiata (L.) Wilczek] genotypes. Legume Research. 42: 585-594
  27. Tahir, A.R.N., Djshwar, D.L., Dlshad, A.O., Shadia, H.S.K., Dastan, A. and Lanja, H. (2018). Genetic diversity and structure analysis of pea grown in Iraq using microsatellite markers. Jordan Journal of Biological Sciences. 11: 201-207.
  28. Uzma, A., Muhammad, J.A., Malik, A.R., Ayaz, A.A., Shahid, I.A. and Anisa, I. (2018). Genetic diversity of pea (Pisum sativum L.) landraces using morphological markers.  International Journal of Biosciences. 13: 327-340. 
  29. Venables, W.N. and Ripley, B.D. (2002). Modern Applied Statistics with S, Springer-rlag.
  30. Yeh, F.C. and Boyle, T.J.B. (1997). Population genetic analysis of co-dominant and dominant markers and quantitative traits. Belgium Journal of Botany. 129: 157.

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