Loading...

Gas Chromatography-Mass Spectrometry (GC-MS) based Metabolomics of Promising Chickpea Genotypes against Callosobruchus chinensis (L.)

DOI: 10.18805/LR-4648    | Article Id: LR-4648 | Page : 1371-1378
Citation :- Gas Chromatography-Mass Spectrometry (GC-MS) based Metabolomics of Promising Chickpea Genotypes against Callosobruchus chinensis (L.).Legume Research.2021.(44):1371-1378
M.S. Sai Reddy, Meena Agnihotri, S.D. Divija, Babita Belal, Somala Karthik mssaireddy@gmail.com
Address : Department of Entomology, Dr. Rajendra Prasad Central Agricultural University, Pusa-848 125, Bihar, India.
Submitted Date : 29-04-2021
Accepted Date : 3-07-2021

Abstract

Background: The pulse beetle, Callosobruchus chinensis L. is one of the main biotic threats to chickpea in both field and storage in the world. The evaluation of chickpea genotypes for pulse beetle resistance has increased motivation for the identification and application of host plant resistance as a critical component of pest management. 
Methods: In the present study, different chickpea genotypes were evaluated under laboratory conditions to identify the antixenosis, antibiosis and GC-MS based untargeted metabolomics of promising chickpea genotypes to the C. chinensis.
Result: Genotype NBeG1004 (36.61 eggs/100 seeds) was less prone to egg laying, while ICCV14872 (180.45 eggs/100 seeds) was the most preferred. However, maximal developmental periods, minimum per cent adult emergence and growth index was observed in ICCV92944 Genotype HC1 showed substantial resistance with maximum phenolic, flavonoid and protease inhibitors content. The results revealed that presence of 9-Octadecenoic acid (Z) in HC1 conferred resistance to C. chinensis. These results show assurance for incorporation into an IPM program against C. chinensis.

Keywords

Callosobruchus chinensis Chickpea GC-MS 9-Octadecenoic acid (Z)

References

  1. Ahmad, J., Maleque, M.A., Islam, M.S. and Bhuiyan, M.A.H.L. (2016). Evaluation of indigenous plant powder against pulse beetle (Callosobruchus chinensis L.) of stored chickpea. Journal of the Sylhet Agricultural University. 3(2): 215-221.
  2. Arora, S. and Kumar, G. (2018). Comparative morphological screening of some species of Cenchrus L. (Poaceae) from Thar Desert of Rajasthan, India. Annals of Plant Sciences. 7(4): 2175-2178.
  3. Chandrasekara, A. and Shahidi, F. (2010). The content of insoluble bound phenolics in millets and their contribution to anti oxidant capacity. Journal of Agricultural and Food Chemistry. 58: 6706-6714.
  4. Chandrasekaran, M., Senthilkumar, A. and Venkatesalu, V. (2011). Anti-bacterial and antifungal efficacy of fatty acid methyl esters from the leaves of Sesuvium portulacastrum L. European Review for Medical and Pharmacological Sciences. 15(7): 775-780.
  5. Deepika, K.L., Singh, P.S., Singh, S.K. and Saxena, R.P.N. (2020). Biochemical basis of resistance against pulse beetle, Callosobruchus chinensis (L.) in stored chickpea geno types. Journal of Experimental Zoology, India. 23(2): 1175-1180.
  6. Ghosal, T.K., Dutta, S., Senapti, S.K. and Deb, D.C. (2004). Role of phenol contents in legume seeds and its effect on the biology of Callosobruchus chinensis. Annals of Plant Protection Sciences. 12(2): 425-475.
  7. Guoqing, L., Zhaojum, H., Lili, M., Xiaoran, Q., Changkun, C. and Yinchnag, W. (2001). Natural oviposition chemicals in female cotton bollworm, Helicoverpa armigera (Hübner). Journal of Insect Physiology. 47: 951-956.
  8. Hajela, N., Sharma, A.H., Sharma, S., Rao, D.N. and Hajela, K. (1999). Studies on a double headed protease inhibitor from Phaseolus mungo. Journal of Plant Biochemistry and Bio Technology. 8: 57-60.
  9. Howe, R.W. (1971).  A parameter for expressing the suitability of an environment for insect development. Journal of Stored Product Research. 7: 63-65.
  10. Jackai, L.E.N. and Singh, S.R. (1988). Screening techniques for host plant resistance to insect pests of cowpea. Tropical Grain Legume Bulletin. 35: 2-18.
  11. Kamble, S.M., Bagde, A.S. and Patil, R.R. (2016). Oviposition pref erence of pulse beetle on different cultivars of chickpea. Journal of Global Biosciences. 5(6): 4197-4201.
  12. Khattab, R.Y. and Arntfield, S.D. (2009). Nutritional quality of le gume seeds as affected by some physical treatments. LWT – Food Science and Technology. 42: 1113-1118.
  13. Kim, D.O., Jeomg, S.W. and Lee, C.Y. (2003). Antioxidant capac ity of phenolic phytochemicals from various cultivars of plums. Food Chemistry. 81(3): 321-326.
  14. Lal, R.R. and Verma, P. (2007). Post-harvest Management of Pulses. Indian Institute of Pulse Research, Kanpur. pp. 26.
  15. Landerito, E.O., Mendoza, E.M., Laurena, A.C. and Garcia, R.N. (1993). Physicochemical and biochemical factors in mungbean [Vigna radiata (L.) Wilczek] and black gram (Vigna mungo) associated with bruchid (Callosobruchus chinensis L.) resistance. Philippine Journal of Crop Science. 18(3): 153-63.
  16. Liu, D.G. and Trumble, J.T. (2007). Comparative fitness of invasive and native populations of the potato psyllid, Bactericerca cockerelli. Entomologia Experimentalis et Applicata. 123: 35-42.
  17. Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951). Protein measurement with the Folin Phenol Reagent. Journal of Biological Chemistry. 193: 265-275.
  18. Malaikozhundan, B. and Thiravia, R.S. (2012). A study on the de velopmental biology of Callosobruchus maculatus (Fabricius) in different pulses. Legume Research. 35(2): 159-163.
  19. Merga, B. and Haji, J. (2019). Economic importance of chickpea: production, value and world trade. Cogent Food and Agriculture. 5: 1615718.
  20. Morgan, L.R., Jursic, B.S., Hooper, C.L., Neumann, D.M., Thangaraj, K. and LeBlanc, B. (2002). Anticancer activity for 4, 4'- dihydroxybenzophenone-2, 4-dinitrophenylhydrazone (A- 007) analogues and their abilities to interact with Lymphoendothelial cell surface markers. Bioorganic and Medical Chemistry Letters. 12(23): 3407-3411.
  21. Raghuwanshi, P.K., Sharma, S., Bele, M. and Kumar, D. (2016). Screening of certain gram accessions against Callosobruchus chinensis L. (Coleoptera: Bruchidae). Legume Research. 39: 651-653.
  22. Reddy, M.S.S. and Agnihotri, M. (2018). Screening of chickpea tolerant genotypes against pod borer Helicoverpa armigera (Hübner) at hotspot Pantnagar. International Journal of Chemical Studies. 6(4): 590-594.
  23. Shaheen, F.A., Khaliq, A. and Aslam, M. (2006). Resistance of chickpea (Cicer arietinum L.)  cultivars against pulse beetle. Pakistan Journal of Botany. 38(4): 1237-1244. 
  24. Sivakumar, R., Jebanesan A., Govindarajan M. and Rajasekar P. (2011). Larvicidal and repellent activity of tetradecanoic acid against Aedes aegypti (Linn.) and Culex quinquefasciatus (Say.) (Diptera: Culicidae). Asian Pacific Journal of Tropi cal Disease. 4 (9): 706-710.
  25. Somta, P., Talekar, N.S. and Srinives, P. (2006). Characterization of Callosobruchus chinensis (L.) resistance in Vigna umbellata (Thunb.) Ohwi and Ohashi. Journal of Stored Products Research. 42(3): 313-327.
  26. Soumia, P.S., Srivastava, C., Dikshit, H.K. and Pandi, G.G.P. (2017). Screening for resistance against pulse beetle, Callosobruchus analis (F.) in greengram [Vigna radiata (L.) Wilczek] accessions. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences. 87(2): 551-558.
  27. Sulehrie, M.A.Q., Golob, P., Tran, B.M.D. and Farrell, G. (2003). The effect of attributes of Vigna spp. on the bionomics of Callosobruchus maculatus. Entomologia Experimentalis et Applicata. 106: 159-168.
  28. Sun, B., Richardo-da-Silva, J.M. and Spranger, I. (1998). Critical factors of vanillin assay for catechins and proanthocyanidins. Journal of Agricultural and Food Chemistry. 46: 4267- 4274.
  29. Tamhane, V.A., Chougule, N.P., Giri, A.P., Dixit, A.R., Sainani, M.N. and Gupta, V.S. (2005). In vivo and in vitro effect of Capsicum annum proteinase inhibitors on Helicoverpa armigera gut proteinases. Biochimica et Biophysica Acta-General Subjects. 1722(2): 156-167.
  30. Zayed, M.Z., Ahmad, F.B., Ho, W. and Pang, S. (2014). GC-MS analysis phytochemical constituents in leaf extract of Neolamarckia cadamba (Rubiaceae) from Malaysia. In ternational Journal of Pharmacy and Pharmaceutical Sciences. 6(9): 123-127.

Global Footprints